Biomedical subjects

R Bhatia

Publications and source records attributed to R Bhatia.

At least 181 records · Page 10Linked to original sources

PubMed1984

Immunodiagnosis of bacterial meningitis.

The quest for the search of rapid, cheap, easy to perform and highly sensitive and specific tests has resulted into the introduction and application of various new tests for the diagnosis of bacterial meningitis. Most of these tests are based on immunological principles, viz. counter-immunoelectrophoresis, latex agglutination test, co-agglutination, radio-immunoassay, haemagglutination inhibition as well as study of immune profile of cerebrospinal fluid. Apart from these certain non-immunological tests viz. Limulus amoebecyte lysate test, gas liquid chromatography, nitroblue tetrazolium dye reduction test have also been evaluated to make the laboratory diagnosis of this important clinical entity rapid and more reliable. These tests have been discussed and their current status presented.

Counterimmunoelectrophoresis↗

PubMed1984

Immunoprophylaxis against rabies.

Rabies is responsible for a large extent of morbidity and mortality in human beings. However, the long incubation period of the disease allows the effective utilization of post exposure immunization. Till recently neural tissue vaccines were the mainstay of antirabic immunoprophylaxis. These vaccines are, associated with severe neuroparalytic complications. The recent introduction of human diploid cell vaccine and chick embro fibroblast vaccines have heralded a new era in antirabic vaccination because of their high immunogenicity and lack of major side effects.

Humans↗

PubMed1984

Antibody response to human diploid cell antirabies vaccine in human volunteers--a preliminary study.

HDCS was given to 12 healthy volunteers in three different schedules. The levels of neutralising antibodies were determined by ELISA test. The antibodies could be detected on day 7 after vaccination. With 3 doses (D-0, D-15, D-30) satisfactory high level of neutralising antibodies could be detected in 3 out of 4 volunteers till 2 years. With 2 doses (D-0, D-15) similar levels could be observed only till day 270, and with single dose significant levels could be seen till day 30 only after which levels declined and no antibodies could be detected on day 270. Reactogenicity of the vaccine was nil as no major or minor side-effects were observed in any of the volunteer.

Adult↗

PubMed1984

Cellulose acetate precipitation test in sero-diagnosis of amoebiasis.

Cellulose acetate precipitation (CAP) test for the detection of antibody against Entamoeba histolytica using the axenic antigen, was performed on the 127 serum samples obtained from patients with amoebic liver abscess (14), amoebic hepatitis (21), amoebic dysentry (11), amoebic colitis (31), other parasitic infestations (25) and normal individuals (25). The percent positivity was 100, 95.23, 90.9, 67.74 and 16 and 12 respectively whereas the corresponding figures for the indirect immunoflourescence (IFAT) test were 100, 100, 100, 74.19, 12 and 8 respectively. Although CAP is not as good a test as IFAT, yet it can be recommended for routine testing due to its sensitivity, speed of performance and applicability to a single serum sample.

Amebiasis↗

PubMed1984