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Biomedical subjects

R Benda

Publications and source records attributed to R Benda.

At least 55 records · Page 3Linked to original sources
PubMed1977

Preparation of ultrathin frozen sections in electron microscopy: specimen supported by polyethylene glycol.

A method was worked out for supporting biological specimens with polyethylene glycol (PEG) for the preparation of ultrathin frozen sections. This method was used in the preparation of ultra-thin cryosections of various tissues and of human diploid cell suspensions infected with vaccina and Herpes simplex viruses. In such preparations it was possible to study stages of virus particle formation under the electron microscope.

Frozen Sections↗
PubMed1976

Electron microscopy of virus particles in ultrathin frozen sections.

A procedure with the use of polyethylene glycol (PEG) 600 to support biological materials for ultracryotomy was developed. Developing and mature virions were demonstrated by electron microscopy in negatively stained frozen sections of vaccinia virus-infected human diploid cells and of herpes simplex virus (HSV) type 1 infected primary rabbit kidney cells. Approximate localisation of the virus particles in the cells was possible.

Culture Techniques↗
PubMed1975

Electron microscopy study of the development of neurovaccinia virus in rabbit blood leucocyte cultures.

In electron microscopy of rabbit blood leucocytes cultured after infection with a large dose of neurovaccinia virus, virus particles can be observed within 24 hours in the cytoplasm of the monocytoid cells (monocytes and macrophages) and occasionally in the polymorphonuclear cells, but never in the lymphocytes. The morphology of the virus particles does not differ from the description of these particles in other types of host cells. The virus replication cycle in the monocytoid cells is slow and the virus matures asynchronously. Virus replication causes infected cells to disintegrate.

Animals↗
PubMed1975

Reproduction of vaccinia virus (strain neurolapina) in rabbit blood leucocytes in vitro.

The determining factor in vaccinia virus reproduction is the initial pattern and subsequent development of the given cell populations, in relation to the infection multiplicity, interferon production and other factors. The sensitive cells in fresh suspensions may be the blood monocytes and the small lymphocytes are virus-resistant. In cultures, transformed lymphocytes (blast cells) and macrophages play the major role in virus reproduction. The macrophages are probably of greater significance for the production of infectious virus. Replication of virus antigen was initiated in the polymorphonuclear cells in exceptional cases only, and only in the presence of a larger infection dose.

Animals↗
PubMed1975

A modified plaque method for arboviruses on plastic panels.

Autoclavable culture media containing an increased (10---15-fold)concentration of succinate buffer permit a comparitively long-term cultivation of cells in free gas exchange with the atmosphere. Based on them, an economical technique of plaque titration of arboviruses on plastic panels with methylcellulose overlay was developed. With seven arboviruses of three different groups and three cell lines (CV-1, PS and PK), the method proved sufficiently sensitive as compared with titrations in mice or tube cell cultures and suitable for plaque reduction tests.

Animals↗
PubMed1975

Experiences with the adaptation of crimean hemorrhagic fever virus to the CV-1 monkey cell line.

Of several cell lines tested, the CV-1 monkey cell line proved to be the most suitable system for propagation of Crimean hemorrhagic fever (CHF) virus. Two CHF strains could be adapted to this cell line by serial passaging. The viral progeny from these passages was specifically cytocidal and produced plaques in CV-1 cells, which could be used in serological diagnosis of CHF. After adaptation of CHF virus to CV-1 cells, the rate of virus synthesis was more rapid, but the yield of virus produced by these cells did not substantially increase. Data reported on cultivation of CHF-Congo viruses are discussed in comparison with the present findings.

Adaptation, Physiological↗
PubMed1975

Electron microscopic demonstration of Crimean hemorrhagic fever virus in CV-1 cells.

Crimean hemorrhagic fever (CHF) virus was detected by electron microscopy in ultrathin sections of CV-1 cells. The virus particles appeared spherical or oval and occurred both intra- and extracellularly. Based on their size and density of their contents, two types of virions, probably representing different phases of the reproduction cycle of CHF virus, could be distinguished.

Animals↗