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Biomedical subjects

R Behl

Publications and source records attributed to R Behl.

4 recordsLinked to original sources
PubMed2026

DNA methylome responses to biotic and abiotic stress in Arabidopsis thaliana: A multi-study analysis.

RATIONALE: Plants experience diverse biotic and abiotic stresses that can induce changes in DNA methylation. However, comparisons among existing studies are complicated by differences in analytical methods and experimental designs. We aimed to identify shared and stress-specific DNA methylation responses across studies. METHODS: We reanalysed 16 whole-genome bisulphite sequencing datasets from 13 Arabidopsis thaliana studies using a unified bioinformatics pipeline. Differentially methylated regions (DMRs) were assessed in the CG, CHG and CHH contexts and examined in relation to genes, transposable elements (TEs), Gene Ontology terms, gene-proximal TE superfamilies and epimutation-prone loci. RESULTS: Global methylation levels were generally stable, whereas the number and genomic distribution of DMRs varied according to stress type and methylation context. CG-DMRs occurred primarily in gene bodies, while CHG- and CHH-DMRs were enriched in TEs. Functional analysis identified shared stress-related processes across conditions. Gene-proximal LINE/L1, RathE1_cons and DNA/HAT elements were enriched for stress-responsive methylation changes. A subset of stress-associated CG-DMRs overlapped loci known to accumulate stable epimutations over generations. CONCLUSIONS: Standardized cross-study analysis revealed both shared and stress-specific methylation patterns. The association of stress-responsive DMRs with gene-proximal TEs and epimutation-prone loci suggests potential links among environmental responses, genome regulation and long-term epigenetic variation.

Arabidopsis thaliana↗
PubMed1992

Early results of direct repair of aortic interruption by the lateral approach.

Eleven consecutive patients were operated on for interruption of the aortic arch by direct aortoplasty through a lateral approach. Median age was 5 days and median weight, 3.52 kg. All received prostaglandin E. Four of the 11 required preoperative ventilatory and inotropic support. Diagnosis was by echocardiography with one confirmed by angiography. There were five type B and six type A interruptions. Aortic continuity was achieved through a left thoracotomy. The aorta and great vessels were mobilized, and after clamps were placed above and below the interruption, ductal tissue was excised and direct anastomosis performed. There was one death from sepsis. One patient died of pulmonary hypertension early after the second operation for correction of other anomalies, and 1 died of hypoplastic lungs after 6 months of ventilation. In 10 of the 11 patients there was no postoperative gradient. Palliative staged approach for correction of this malformation is advocated. The need for bypass and profound hypothermia is removed. This approach restores aortic continuity without sacrifice of the major vessels, use of ductal tissue, or use of prosthetic grafts that will necessitate reoperation.

Aorta, Thoracic↗
PubMed1989

In vitro capacitation of goat spermatozoa.

Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.

Acrosome↗