Extracorporeal CO2-removal with a heparin coated extracorporeal system.
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Carbon Dioxide↗
Biomedical subjects
R Bauer
Publications and source records attributed to R Bauer.
A quantitative assay for neomycin phosphotransferase activity in plants.
A sensitive, simple, and quantitative assay for determining neomycin phosphotransferase (NPT) activity in plant cell extracts is described. The procedure retains the simplicity of previously published methods, yet offers up to a 140-fold increase in sensitivity. This increase is due to (1) the addition of bovine serum albumin (BSA) to the assay mixture, (2) desalting of crude maize extracts to remove a low-molecular-weight inhibitor of the enzyme, and (3) use of a different extraction buffer and an improved extraction procedure to liberate more enzyme from the cells. This method has been used successfully to detect and quantitate both stable and transient expression of NPT in transgenic tobacco and maize tissue.
Animals↗
Determination of regional blood flow in abdominal organs and other structures in normal female rats and in rats with TAA-induced chronic liver injury using 99mTc labelled HSA-microsphere technique.
A method for simultaneous determination of cardiac output and regional blood flow distribution in a chronically instrumented, unrestrained rat preparation for different experimental conditions (i.e. conscious, free movement; general anesthesia) and in an experimental chronic liver injury model is described. The use of a modified radioactive microsphere reference sample method using 99mTc labelled HSA-microspheres provides valid measurements of cardiac output [255 +/- 21.6 ml/(min.kg b.wt.)] and of the determined blood flow rates of abdominal organs (with separate determination of arterial and portal-venous hepatic blood flow rates; the latter by means of arterial blood flow measurement of the gastrointestinal tract and the spleen), the myocard, the adrenals, the kidneys, and various brain regions. Furthermore, it is demonstrated that this measuring approach with chronical preparation can also advantageously be used in pharmacological or pathogenetical studies, especially because of the simple measuring equipment and the comparatively low costs that offer a broader application.
Abdomen↗
[The weighting of SE, IE and GE sequences in tumors of the head and neck].
In a review of 82 patients having different benign and malignant head and neck tumours we studied the value of spin echo (SE), inversion echo (IE) and gradient echo (GE) sequences. The strong T1 weighted IE mode is highly sensitive to demonstrate pathological tissue. Tumour diagnosis was in 20% and lymph node diagnosis in 29% better than T2 weighted SE sequences. According to this experience we choose the IE mode as the first one for all patients examined for tumour staging. All other sequences and orientations are planned on this tomograms knowing the location of the tumour and lymph nodes. T1 weighted SE mode is indispensable and should be recorded in transversal orientation, covering the whole head and neck region. This mode has the best imaging of anatomical structures as the known fatty limitations. The best differentiation of the tumour is usually obtained after contrast enhancement by i.v. Gd-DTPA. The highest contrast enhancement is seen on T1 weighted GE tomograms, which we prefer. T2 weighted SE sequences are less sensitive than IE sequences and T1 weighted tomograms after Gd-DTPA to show the tumour. The T2 weighted SE mode cannot be replaced by the T2 weighted GE mode having a better signal to noise ratio. To minimise the scan-time of T2 weighted SE mode, only one measurement is performed; the quality of the tomograms is satisfactory.
Adult↗
[Initial experiences with transpopliteal, laser-assisted balloon angioplasty of femoral artery occlusions].
For many years great efforts are being made to recanalise totally occluded vessels and averting a bypass operation as long as possible. In two years 190 patients were treated successfully by the Lastac System. Encouraged by very good results, we started to recanalise the totally occluded superficial femoral artery via a transpopliteal approach. Meanwhile, 29 patients were treated. Primary successful were 22 patients with an average length of a total occlusion of 27.5 cm.
Adult↗
Phenotypic and functional characterization of tumor infiltrating lymphocytes in mycosis fungoides: continuous growth of CD4+ CD45R+ T-cell clones with suppressor-inducer activity.
Tumor-infiltrating lymphocytes (TIL) were obtained by mechanical release from a solitary rapidly grown tumor of a patient with mycosis fungoides. The preparations separated by density gradient centrifugation contained a major portion of CD3+ CD8+ WT31+ CD5- large T-cell blasts and a minor portion of non-blastic TIL predominantly of the CD3+ CD4+ phenotype. Using cDNA-probes for the constant region of the T-cell receptor beta-genes, the large cell fraction was identified as tumor by its distinct monoclonal rearrangement. TIL were expanded by culture in recombinant interleukin 2 and cloned by limiting dilution. Phenotypic analysis of expanded TIL and two clones further analyzed in more detail showed CD3+, CD4+, CD8-, and 2H4+ (CD45R+) expression. Cloned and uncloned TIL showed no NK and LAK activity, no proliferative response, and no cytotoxic activity against autologous tumor cells. These cells were unable to suppress the proliferative response of alloreactive T-cell clones stimulated by B-lymphoblastoid cell lines (i.e., they had no suppressor-effector activity), but strongly suppressed proliferation responses in allogeneic mixed lymphocyte culture (i.e., they most likely had suppressor-inducer activity). This was not the case when irradiated tumor cells were added. The present results demonstrate continuous in vitro growth of CD4+ and 2H4+ T-cell clones with suppressor-inducer activity obtained from TIL, and indicate that a subpopulation of TIL may down-regulate immune responses which may lead to suppression of antitumor immunity.
Antigens, CD↗
[Acrosyringium and follicular infundibulum: immunomorphologic aspects].
Acrosyringium and infundibulum are epidermal structures with different morphology, but functional similarities regarding re-epithelialization, transport of antigens, and attraction of infiltrates. Immunomorphologically, the infundibula revealed an immunoreactivity of Langerhans cells and keratinocytes comparable with that of the interfollicular epidermis, whereas the acrosyringia reacted differently because of the permanent expression of MHC class II antigens and reduced Langerhans cells in the perisyringial region. Our findings suggest that these epidermal compartments differ with regard to their individual immunologic situations. While the mechanisms of epidermal immunity known from the interfollicular epidermis are possibly the same in the infundibula, we have to expect a basically different immunologic situation in the acrosyringia.
Antigens, CD1↗
[Percutaneous radio-frequency sympathetic block in peripheral circulatory disorders].
Thirty patients with inoperable diabetic angiopathies (degree III and IV concerning to Fontaine) were treated by means of percutanous radiofrequency thermolesion of the lumbar sympathetic chain (PRFS). All patients reported immediately after this procedure a distinct feeling of warmth in the treated leg. After a period of 6 month to two years 22 patients were re-examined clinically: Fifteen patients changed to Fontaine IIa and ten patients were free of further complaints. Also an improved wound healing and ulcer demarcation following amputation or skin transplantation was observed. The advantage of PRFS in comparison to the surgical sympathectomy is the fact that there is no general anesthesia necessary, which too minimizes the risk especially for the aged vascular patient. Moreover the patient can be mobilised immediately after the procedure. There were no complications like lesion of the ureter or the genitofemoral nerve as well as bleading into the psoas muscle as reported in percutanous chemical lesion of the sympathetic chain.
Adult↗
[Differential behavior of skin temperature in vascular patients vs. pain patients in lumbar radicular stimulation and nerve block].
Plantar skin temperature monitoring during the procedure of percutaneous radiofrequency-thermolesion of the sympathetic chain (PRFS) in patients with vascular diseases and patients with lumbar radicular pain and signs of sympathetic irritation showed a distinct difference. A significant decrease of skin temperature was observed during stimulation of the sympathetic chain by means of canula placement and contrast medium instillation only in the group of "vascular" patients, while "radicular pain" patients showed a significant increase of skin temperature after PRFS. This observation leads to the conclusion that the effect of sympathectomy more or less is to prevent physiological hyperactivity of the sympathetic system.
Adult↗
[Laser-assisted balloon angioplasty--results and complications of the first 200 treated cases].
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Aged↗
[Brain stem and heart rate fluctuations--experimental and clinical results for topical mapping and selective quantification].
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Animals↗
[Recanalization of peripheral vascular occlusions using an argon laser].
A new Argonlaser developed in the United States of America is available to the radiological and surgical departments of the Hospital of St. Pölten. It is used for recanalization of totally occluded peripheral arteries. During the last 9 months, 51 patients (30 men and 21 women) with an average age of 71 years have been treated. Total occlusion was present in 88% of the arteries treated. The primary success rate is 89%, and the perforation rate is under 2%.
Adult↗
[99mTc-2-methoxy-isobutyl-isonitrile in the diagnosis of coronary heart disease: results of a multicenter study].
A total of 226 patients was studied in a phase-III multicenter trial to compare the results of stress/rest 99mTc-MIBI with 201TI scintigraphy and/or coronary arteriography. The evaluation of planar and SPECT imaging was based on a semiquantitative visual scoring. A congruent diagnosis was generated from all planar 201TI and 99mTc-MIBI scintigrams performed in 36 patients. In 62 of 63 patients, SPECT-studies with 99mTc-MIBI and 201TI led to an identical diagnosis. In one patient a positive result was obtained with 201TI-SPECT whereas 99mTc-MIBI was negative. Segmental agreement for 1509 SPECT segments was 85.7%, for 554 planar segments it was 86.6%. In comparison to coronary arteriography, performed in 180 patients, the overall sensitivity for the correct diagnosis of coronary artery disease (CAD) was 86% for planar MIBI scintigraphy or 92% for MIBI-SPECT. Overall specificity was 100% for planar imaging and 57% for SPECT. The low specificity of MIBI-SPECT was probably due to high prevalence of CAD in this study population. In a subgroup of 43 patients, who underwent coronary angiography, identical results were found with 99mTc-MIBI and 201TI. Only one patient showed a positive scintigraphic result with both 99mTc-MIBI and 201TI without angiographically proven stenosis greater than 50% or prior myocardial infarction. Vessel sensitivities in stenosed coronary arteries (greater than 50% stenosis) were 54% for the LAD and 87% for the RCX/RCA areas with MIBI-SPECT, or 51% or 79% for planar scintigraphy, respectively. Vessel specificities for SPECT were 65% in the LAD or 52% in the RCX/RCA, or 90% or 83% in planar imaging.(ABSTRACT TRUNCATED AT 250 WORDS)
Adult↗
[The Luque-Galveston operation in the treatment of neuropathic pelvic tilt].
A series of 17 patients with neuromuscular pelvic obliquity underwent Luque-Galveston instrumentation. None of these patients had an increased neurologic deficit. One intraoperative death occurred due to pulmonary air embolism. It was possible to follow up 13 patients for an average of 2.7 years. The average correction achieved was from 97.6 degree to 38.8 degree for pronounce unbalanced lumbar or thoracolumbar curves: the correction loss averaged 3.6%. Combination with anterior correction or fusion in 38% is believed to be the factor that enabled us to reduce the incidence of pseudarthrosis and implant failure.
Adolescent↗
[Magnetoencephalography--current trends].
The review deals with the method of magnet-encephalography. The physical principles and the most important technical conditions to put into practice this method are mentioned. Comparing with the electroencephalography this method shows various advantages, what leads to its intensive use for the localisation of components of evoked potentials, for the magnet-encephalographic record of the brain activity of the human fetus, and for the localisation of epileptic foci in therapy resistant epileptics.
Brain Damage, Chronic↗
[Indications for and results of surgical scoliosis therapy using VDS instrumentation (ventral derotation spondylodesis)].
The complications of 97 patients who were operated with anterior spinal instrumentation were frequent, but insignificant. 22 of these patients with lumbar and thoracolumbar curves in whom the VDS instrumentation was used were reviewed. A 79% correction was obtained in the coronal plane. Loss of correction was 11% in the first year and further 2% at 56 months average follow up. In the sagittal plane a mild tendency to increased kyphosis was found. A permanent "adding on" phenomenon occurred in one case. The VDS-instrumentation appears the apparatus of choice for treatment of thoracolumbar and lumbar scoliosis, other indications are discussed.
Adolescent↗
Physiochemical features of monoclonal idiotype-anti-idiotype complexes: importance of inter-chain disulfides for size distribution patterns and molecular geometries.
Cyclic tetramers represent the preferentially formed complexes of a murine monoclonal idiotype-anti-idiotype (Id-anti-Id) system consisting of IgG antibodies or (Fab')2 fragments at micromolar concentrations. The cleavage of inter-chain disulfides of both Id and anti-Id caused the predominant generation of cyclic dimers at the expense of larger aggregates, suggesting with regard to already published data that the hinge located interheavy-chain disulfides are essential for the strain.
Antibodies, Anti-Idiotypic↗
Hemodynamic changes after an intensive short-term exercise and nutrition program in hypertensive and obese patients with and without coronary artery disease.
PURPOSE: To assess the effectiveness of the Pritikin diet and exercise program on cardiovascular hemodynamics using the noninvasive technique of Thoracic Electrical Bioimpedance (TEB). MATERIAL AND METHODS: Twenty subjects divided in two groups, according to their body habitus and hemodynamic disturbances. These data were compared to a group of 10 healthy individuals not involved in the program. Hemodynamic parameters were collected at admission and at the end of the intensive 26-day program of exercise and nutrition. RESULTS: In obese and hypertensive subjects not on medication we observed that cardiac index increased from 3.27 +/- 0.4 to 3.58 + 0.5 L/min/m2; mean arterial pressure decreased from 100 +/- 8.5 to 94.8 +/- 7.9 mmHg while systemic vascular resistance index decreased from 2362 +/- 391 to 1934 +/- 357 dynes. sec. cm-5/m2; p less than 0.05 (Data obtained in supine position). Also documented was a improvement in ventricular performance after postural changes from upright to supine based on indices of left ventricular performance, uniquely obtained by the TEB technique. From admission to discharge, changes were: Ejection fraction 48% to 53%; Peak flow index 295 to 316 ml/s/m2 and Index of contractility 40 to 47 s-1, explained by a shift on the ascending limb of the Starling curve. CONCLUSION: In a selected population, this rehabilitation program is effective for hemodynamic improvement that can be partially explained by metabolic and biochemical changes already reported from this Center.
Adult↗