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Biomedical subjects

R B Wilson

Publications and source records attributed to R B Wilson.

At least 127 records · Page 7Linked to original sources
PubMed1971

Further modification of a technique for radioimmunoassay of insulin.

Since our original report we adapted a modification of the immunoassay procedure that increased the sensitivity and reproducibility of the assay. The differences between the present procedure and the previous one are: (a) an initial 20 hour incubation period before the addition of the tracer insulin, and (b) incubation at 37 degrees C instead of 4 degrees C. The adapted method resulted in standard curves of similar slope for bovine, porcine, rhesus monkey, canine and human insulin. The validity of the assay for bovine insulin was established by recovery and dilution experiments.

Animals↗
PubMed1971

Blood vessel tumorigenesis by 1,2-dimethylhydrazine dihydrochloride (symmetrical). Gross, light and electron microscopic descriptions. I.

Administration of 0.001% 1,2-dimethylhydrazine dihydrochloride, symmetrical, in the drinking water of 7-week-old randomly bred Swiss mice for the remainder of their lifetime induced blood vessel tumors and enhanced the incidence of lung neoplasms. Ninety-eight percent of the females and 92% of the males developed vascular lesions, whereas among the controls the incidence was 3% in the females and 1% in the males. In addition, the incidence of lung tumors rose from 12 to 44% in the females and from 10 to 24% in the males, as compared with the controls. The occurrence of the vascular tumors in order of decreasing frequency was as follows: muscle, pararenal, fat, liver, parametrial, paraepididymal tissues, etc. Gross, light and electron microscopic examinations of vascular lesions revealed the characteristic appearance of angiosarcomas. The type and extent of macroscopic and histologic involvements of the various tissues by the tumors are presented. The ultrastructural descriptions of hemorrhagic areas, vascular spaces, neoplastic endothelial cells, their cytoplasms and organelles are illustrated in detail.In conclusion, whereas hydrazine enhanced the development of lung tumors, when the dimethyl group was attached to it at symmetrical positions, it evoked vascular tumors. Thus, the present study provides evidence for the possible relationship between chemical structure and tumor induction at specific organ sites.

Animals↗