Biomedical subjects
Publications and source records attributed to R B Tenser.
Continuous unilateral facial myokymia and facial contraction occurred in a patient with multiple sclerosis and there was subsequent resolution of these abnormalities. I compare abnormal facial movements of this type with those caused by other neurological diseases and discuss the possible mechanism of myokymia in diseases of the central and peripheral nervous systems.
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Dorsal root ganglion neurons, which express herpes simplex virus (HSV) latency-associated transcript (LAT) during experimental latent infection, were investigated by in situ hybridization. The number of LAT-positive neurons was determined by examination of ganglion serial sections. In other latently infected mice, the number of ganglion neurons that reactivated HSV antigen after explant culture was determined in serial sections. LAT was detected in 100 percent of ganglia, with an average of 19.5 LAT-positive neurons per ganglion. After explant culture of latently infected ganglia (in the presence of colchicine to decrease spread of reactivated virus), HSV antigen was detected in 94 percent of ganglia, with an average of 13.1 positive neurons in the antigen-positive ganglia. The similar quantities of LAT- and antigen-positive neurons within ganglia support the hypothesis that LAT-positive neurons were the neurons from which HSV was reactivated.
Latent herpes simplex virus (HSV) infection of the trigeminal ganglion of guinea pigs and latent varicella-zoster virus (VZV) infection of the trigeminal ganglion of humans were studied by in situ nucleic acid hybridization. Guinea pig trigeminal ganglia were removed during the period of viral latency (four to five weeks after corneal inoculation of HSV), and human ganglia were removed at autopsy. Radiolabeled HSV and VZV DNAs were used to probe ganglion tissue sections for viral-specified RNA. Hybridization detected only over neurons was present in 46 percent of ganglia from 22 latently infected guinea pigs and from 33 percent of ganglia from 10 human subjects. These results support the conclusion that some viral transcription occurred during HSV and VZV latency.