Localization of chlorophyll in spinach chloroplast lamellae by fluorescence microscopy.
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Biomedical subjects
Publications and source records attributed to R B Park.
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The assimilation of CO(2) by etiolated Hordeum vulgare seedlings during an illumination period indicates a conversion of the organisms to autotrophy.After 1 hour illumination, increases in the photo-assimilation of CO(2) are observed and the distribution of C(14) in the soluble fraction of the plants is predominantly in intermediates of the Calvin cycle.
Both lipase from runner beans and a protease (pronase) initially cause increased intensity dependence of the DCPIP Hill reaction of spinach chloroplasts. This is followed by an increase in the extrapolated zero intensity quantum requirement. Pronase treatment of the chloroplasts causes rapid changes in absorption and ORD spectra, whereas the effect of lipase treatment is much less pronounced. Long treatments of the thylakoids with the lipase and protease cause unique morphological changes within the membrane. These changes are used to assign chemical compositions to some of the structures revealed by freeze-etching.
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(1) Chloroplast lamellae, like many other membranes, are composed of 90-A subunits. (2) The way in which the 90-A units associate with one another determines the quantasome structures of the membrane. There appear to be four 90-A subunits per quantasome in the most highly organized arrays. In at least some cases, the way in which the subunits bind one to another is under environmental control. (3) Extraction experiments are consistent with a model in which the membrane lipid surrounds the protein framework of the membrane.
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