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Biomedical subjects

R B Davey

Publications and source records attributed to R B Davey.

At least 91 records · Page 5Linked to original sources

The burn clinic.

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Australia↗

Epidermoid cyst of the appendix.

Epidermoids cyst of the abdominal viscera is an uncommon condition. Cases have been reported involving the spleen, kidneys, gonads, fallopian tubes, uterus, mesentery, and cecum (mice). An epidermoid cyst of the vermiform appendix is presented.

Appendiceal Neoplasms↗

Endonuclease fingerprinting of plasmids mediating gentamicin resistance in an outbreak of hospital infections.

Three plasmids which each determined the same extensive antibiotic resistance phenotype, including resistance to gentamicin, and which had been recovered from organisms involved in an outbreak of gentamicin-resistant infections in Melbourne hospitals in 1975--1976, were analysed by fingerprinting with the restriction endonucleases Eco RI, Hind III and Pst I, In each case the fingerprints for all three plasmids were identical, despite the fact that each plasmid originated in a different host species at different hospitals. This observation confirms our earlier hypothesis (Davey and Pittard, 1977) that the plasmids mediating gentamicin resistance in the outbreak were the closely related descendants of one ancestral (IncL) plasmid.

Cross Infection↗

Plasmids mediating resistance to gentamicin and other antibiotics in Enterobacteriaceae from four hospitals in Melbourne.

In twenty-nine gentamicin-resistant strains (mostly Klebsiella species) isolated from four Melbourne hospitals over a six month period, the genes for gentamicin resistance were carried on conjugative plasmids belonging to the rare incompatibility group L. These plasmids conferred resistance to gentamicin and sisomycin but not to amikacin or tobramycin, and were inferred to specify the enzyme aminoglycoside 3-N-acetyltransferase (AAC(3)I or GAT I). In addition, they specified resistance to a number of other antibiotics, including ampicillin and carbenicillin, chloramphenicol, kanamycin and neomycin, spectinomycin and streptomycin, sulphonamides and tetracyclines. Although they had a wide host range amongst the Enterobacteriaceae, none of these plasmids appeared able to transfer to Pseudomonas.

Anti-Bacterial Agents↗

Lymphosarcoma of bowel in childhood.

A report of nine cases of lymphosarcoma of the bowel in childhood is presented, together with a review of the literature. Until recently, the prognosis of this condition has been poor,but it is hoped that modern treatment with surgery, radiotherapy and multiple cytotoxic drugs given in high dosage over a two-year period will result in improved results.

Child↗

Potential for in vivo acquisition of R plasmids by one strain of Vibrio cholerae biotype El tor.

The feces of five patients admitted to a hospital during an outbreak of cholera in Melbourne, Australia, in November 1972, were examined for the presence of tetracycline-resistant coliforms and tetracycline-resistant strains of Vibrio cholerae. Despite the abundance of tetracycline-resistant coliforms able to transfer this resistance to other strains of Escherichia coli, no tetracycline-resistant strains of V. cholerae were detected. In vitro transfer experiments using the V. cholerae strain responsible for the outbreak as recipient revealed that it was a particularly poor host for most R plasmids.

Cholera↗

Genetic and biophysical study of R plasmids conferring sulfonamide resistance in Shigella strains isolated in 1952 and 1956.

The conjugative plasmids determining sulfonamide resistance in five Shigella strains, each isolated from a different patient, have been characterized. One S. flexneri 2a strain, isolated in 1952, harbored an fi(+) plasmid of molecular weight 53 x 10(6), which specified synthesis of F-like pili and bore determinants for sulfonamide resistance (Su) and bacteriocinogeny (Col). This plasmid was compatible with plasmids of groups F(I), F(II), I(alpha), and P. A second S. flexneri 2a strain isolated in 1952 harbored an fi(-) plasmid of molecular weight 59 x 10(6), bearing the Su determinant and compatible with all plasmids tested. This strain also harbored an fi(+) group-F(II) plasmid of molecular weight 42 x 10(6), which bore the Col determinant and specified synthesis of F-like pili. Three S. dysenteriae 2 strains isolated in 1956 carried apparently identical fi(-) plasmids of molecular weight 58 x 10(6), which bore the Su determinant, could form transconjugants in Pseudomonas but not in Proteus, and were incompatible with the P-group plasmid RP4.

Australia↗