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Biomedical subjects

R Anderson

Publications and source records attributed to R Anderson.

At least 667 records · Page 37Linked to original sources
PubMed1979

Effects of ascorbate on leucocytes: Part IV. Increased neutrophil function and clinical improvement after oral ascorbate in 2 patients with chronic granulomatous disease.

A brother and sister with chronic granulomatous disease (CGD) of the autosomal recessive type, and with markedly defective neutrophil motility and elevated serum IgE levels, were treated with a single oral daily dose of 1 g ascorbate for 6 months. Neutrophil function and serum IgE levels were measured repeatedly at approximately monthly intervals. Both children also received prophylactic antibiotics which were always stopped 1 week prior to testing of immune function. Ascorbate treatment was accompanied by significantly increased neutrophil motility and post-phagocytic metabolic activity, and a reduction in serum IgE levels. Enhanced neutrophil function correlated with clinical improvement. Both children have remained free of infection since ascorbate was added to their regimen and have gained weight.

Ascorbic Acid↗
PubMed1979

Effects of ascorbate on leucocytes: Part II. Effects of ascorbic acid and calcium and sodium ascorbate on neutrophil phagocytosis and post-phagocytic metabolic activity.

The effects of ascorbic acid and calcium and sodium ascorbate at a concentration range of 10(-6)M - 10(-1)M on polymorphonuclear leucocyte (PMN) phagocytosis of Candida albicans and post-phagocytic nitroblue tetrazolium (NBT) reduction, hexose monophosphate shunt (HMS) activity and myeloperoxidase-mediated iodination of ingested protein were investigated. Phagocytosis of C. albicans was unaffected by ascorbate concentrations of 10(-6)M - 10(-2)M; however, progressive inhibition was observed at concentrations of 10(-2)M upwards. Enhancement of resting and stimulated HMS activity and NBT reduction was evident at ascorbate concentrations of 10(-5) M - 10(-2)M. The stimulations of HMS activity and NBT reduction was independent of myeloperoxidase iodination of ingested protein and this latter function was strongly inhibited by ascorbate. Concentrations of ascorbic acid and calcium and sodium ascorbate which caused inhibition of phagocytosis and HMS activity were the same as those which mediated stimulation of cell motility, indicating that independent cellular mechanisms may govern motility and phagocytosis.

Ascorbic Acid↗
PubMed1979

Studies on the biosynthesis of sulfolipids in the Diatom Nitzschia alba.

Labeling of sulfolipids in Nitzschia alba was studied after growth of the cells in media containing L-[35S]cystine, L-[35S], L-[35S]cysteine, L-[35S]-methionine or a mixture of L-[Me-3H]methionine and L-[35S]methionine, [35S]Cysteine or [35S]cystine labeled the deoxyceramide sulfonate and the sulfonium analog, phosphatidylsulfocholine (and its lyso derivative) but not the sterol sulfate nor the sulfoquinovosyl diglyceride; [35S]methionine labeled only the phosphatidylsulfocholine and its lyso derivative. With the [35S]- and [Me-3H]methionine mixture (3H/35S ratio 1.0) the phosphatidylsulfocholine had a 3H/35 S ratio of 1.5 indicating that both sulfonium methyl groups were derived from methionine. Probable biosynthetic pathways for these novel sulfolipids are discussed.

Amino Acids, Sulfur↗
PubMed1979

Effects of metronidazole on certain functions of human blood neutrophils and lymphocytes.

The effects of metronidazole at varying concentrations (10(-7)M - 10(-2)M) on certain cellular immune functions were assessed in vitro. Neutrophil chemotaxis to endotoxin-activated autologous serum, random motility and postphagocytic metabolic activity (hexose monophosphate shunt activity, myeloperoxidase-mediated protein iodination and glycolysis) were unaffected. Likewise, metronidazole had no detectable effects on lymphocyte-active E-rosette formation, mitogen-induced DNA synthesis and mitogen-induced protein synthesis.

Blood Proteins↗
PubMed1979

Public awareness and interest in community health councils.

Community health councils were set up four years ago. They were an institutional innovation, with a remit to represent local community interests in the health services to those responsible for managing them and to facilitate communication between management and (potential) users of the health service. The cover of one CHC's annual report explains the service offered: 'We can make sure that the consumer's point of view is heard. We can help people who need information about the NHS and those who have a complaint about something which has gone wrong'. They might have added, as a caveat, that to do all this effectively, 'we need you'. This paper presents some information, from a recent national study of general practice (Cartwright and Anderson), about public awareness of an interest in community health councils.

Community-Institutional Relations↗
PubMed1979

The in vitro effects of propranolol and atenolol on neutrophil motility and post-phagocytic metabolic activity.

Propranolol at concentrations of 1 x 10(-6) to 1 x 10(-4) M consistently increased neutrophil motility as measured in Boyden chambers. The effects were not due solely to stimulation of random migration and chemokinesis but also of directional motility. Propranolol, over a similar concentration range, caused inhibition of post-phagocytic cell metabolic activity (hexose monophosphate shunt, nitro-blue tetrazolium reduction and protein iodination) without any detectable effect on the ingestion rate of Candida albicans. Atenolol had no effect on any of these neutrophil functions. Both drugs were without effect on glycolysis and intracellular cyclic AMP levels. Propranolol however, at concentrations which stimulated cell motility, caused increased intracellular cyclic GMP levels. It is suggested that propranolol may stimulate neutrophil motility by promoting increased intracellular cyclic GMP levels or by decreasing neutrophil superoxide production.

Atenolol↗
PubMed1979

The in vitro evaluation of certain neutrophil and lymphocyte functions following the ingestion of 150 mg oral dose of levamisole: assessment of the extent and duration of stimulation of neutrophil chemotaxis, protein iodination and lymphocyte transformation.

Certain functions of human blood neutrophils and lymphocytes were investigated at varying time intervals after the ingestion of a single 150 mg dose of levamisole. The functions tested were neutrophil chemotaxis and post-phagocytic metabolic activity and mitogen-induced DNA and protein synthesis of lymphocytes. It was found that levamisole causes a stimulation of neutrophils motility (cell- and serum-associated) and post-phagocytic hexose monophosphate shunt activity and protein iodination. Increased lymphocyte DNA synthesis, but not protein synthesis, to the mitogen phytohaemagglutinin was observed. The stimulation which was detected almost immediately of these neutrophil and lymphocyte functions was still evident 24 hr later but not at 48 hr, indicating that a single oral dose of levamisole can cause the alteration (stimulation) of leucocyte functions which persists until 24--48 hr after intake of the drug.

Chemotaxis, Leukocyte↗