Search PubMed⌕ Search

Biomedical subjects

R Alonso

Publications and source records attributed to R Alonso.

224 records · Page 13Linked to original sources
PubMedDate not supplied

[Daily rhythms of melatonin in pineal, serum and urine of rats (author's transl)].

The levels of melatonin in urine, serum, and pineals were determined in rats during the light/dark cycle. Previous to the radioimmunoassay, melatonin was isolated using a new ascending chromatographic method with programmed multiple development. The daily excretion of melatonin and its serum levels showed a characteristic rhythm, night samples containing several times more melatonin than those of the light period. The pineal content of melatonin of animals killed at different intervals also showed a similar day/night rhythm. The chromatographic method used gave a perfect separation of melatonin from other materials which could interfere in the assay. The mobility of material detected by RIA was identical to that of authentic melatonin chromatographed concurrently.

Animals↗
PubMedDate not supplied

Specific IgE determination using the CAP system: comparative evaluation with RAST.

The CAP system's main contribution is its solid phase, which consists of a cellulose polymer activated within a capsule (the ImmunoCAP). This solid phase can bind more protein to it, and, in addition, the conditions of reaction seem to make the system more sensitive at detecting antibodies to certain antigens. It is therefore important to assess the new analytical and diagnostic performance in comparison with previous systems. In this context, we studied the reliability and comparison with the RAST and with skin tests carried out on 144 pediatric patients. Skin tests and specific IgE for radioimmunological RAST (radio-allergosorbent test) and for the fluoroimmunological CAP system were performed on all the patients. The RAST/CAP correlation quotients for the different allergens tested varied between 0.971 and 0.991. Diagnostic sensitivity increased for all the allergens studied and specificity remained unchanged. The system provides reliable results, with better diagnostic capacity than RAST, but it must be quantified for each allergen because its results are not interchangeable.

Adolescent↗
PubMedDate not supplied

Annual, daily and diurnal variations in pollen from Olea europaea L. in the atmosphere of Granada (Spain).

The allergenic capacity of Olea europaea L. pollen is very important in several cities of the southern Iberian Peninsula, producing most pollinoses diagnosed in these cities. We have carried out a study on the annual, daily and diurnal variations in pollen from O. europaea in the atmosphere of Granada (Spain) during 4 consecutive years. Samplings were carried out with the aid of a Burkard spore trap. Very low levels of Olea pollen grains were first detected in the atmosphere from the end of April. Pollen levels peaked in May and June, and started to decrease from the last days of June and in July. Pollen in the atmosphere is related to meteorological parameters: temperature has a positive influence on pollinization and rainfall produces a negative effect. The diurnal variation pattern of pollen grain concentrations was quite homogeneous throughout the study, featuring an important increase around midday and a moderate decrease in the first hours of the morning and in the late evening.

Air Pollution↗
PubMedDate not supplied

[Transrectal recatheterization under echocardiography control].

We present the case of a 36 years-old woman, in whom antegrade mitral intraluminal valvulotomy was performed under transesophageal echocardiography control. Inadvertently, we retired the Inoue's catheter to the right atrium from the left atrium, before the dilation of the mitral valve was accomplished. Under echocardiographic control we reintroduced the transseptal catheter across the former septal orifice, avoiding a new septal puncture, and its possible complications. Now a days, the antegrade intraluminal mitral valvulotomy is easy performed, because the transesophageal echocardiography monitoring. The transesophageal echocardiography has modified the transseptal catheterization contraindications.

Adult↗
PubMedDate not supplied

[Effectiveness of anticoagulant oral treatment in patients with thrombus in left ventricle after acute myocardial infarction].

Left ventricular mural thrombi (LVMT) is a complication of acute myocardial infarction (AMI), that may produce peripheral embolism which could be fatal. In order to establish an adequate time of oral anticoagulant (OA) therapy, we undertook a prospective study that included 45 patients with AMI and left ventricular thrombi detected by echocardiographic study, in the first 5 to 10 days postinfarction, the study was repeated, in 3 and 6 months. Treatment with oral anticoagulant was initiated at the point of the detection of thrombi maintaining an INR of 1.5 to 2. Thirty nine patients (79%) were males and 6 (11%) were females, with an age of 29 to 85 years and a range of 62 +/- 11 years. Forty four patients (98%) presented anterior wall infarction and 1 (2%) posteroinferior infarction. In patients with anterior infarction, in 38 (85%) the thrombi was located at the apical wall (p < 0.05), 5 (11%) in the septal wall and other (2%) in anterior and apical walls. The patient with the posteroinferior infarction presented extension to the right ventricle, where the thrombus was located (2%). The contractility alterations related with thrombi were diskinesia, followed by hipokinesia and finally akinesia. The ejection fraction had not relationship with thrombi formation. LVMT dissolved in 32 patients (71%) at 3 months (p < 0.05), in 8 (18%) in 6 months and in 5 (11%) it was maintained for more than 6 months. None of the patients presented complications of OA. We conclude that the LVMT are more frequent in anterior infarctions, essentially in those that present diskinesia. The majority of LVMT are resolved in 6 months with OA therapy.

Adult↗