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Biomedical subjects

R Alon

Publications and source records attributed to R Alon.

74 records · Page 5Linked to original sources

Hemisplenectomy using a hand-held CO2 laser. An experimental study.

Hemisplenectomy was performed in mongrel dogs using a hand held CO2 laser. Cutting and hemostasis were performed by a laser beam with a high concentration power--about 6kW/mm2--at the focal spot, and residual bleeding was controlled by using a defocused beam. The cutting action was improved by temporary closing of the arterial vessels and by injecting adrenaline into the splenic artery to produce a relatively dry surface. The surgical procedure was free of mortality or morbidity. Histological studies revealed a brand of fibrous tissue at the cut surface, without apparent abnormalities in the splenic parenchyma beneath the section. Activity of the residual half spleen was shown by postoperative technetium scan and by the fact that Howell-Jolly bodies appeared in peripheral blood only after resection of the remaining half spleen.

Animals↗

Tumor cell MUC1 and CD43 are glycosylated differently with sialyl-Lewis a and x epitopes and show variable interactions with E-selectin under physiological flow conditions.

The mucins secreted from the colon carcinoma cell line COLO 205 have the MUC1 and CD43 (leukosialin) as core proteins, where both carry sialyl-Lewis a and MUC1 sialyl-Lewis x epitopes. The adhesion of E-selectin expressing CHO cells to the coated mucins was analyzed in a flow system revealing that the MUC1 mucin adhered better than the CD43 mucin. One reason could be their different glycosylation, a difference that was explored by analyzing the biosynthesis of MUC1 and CD43 in COLO 205 cells. Both the MUC1 and CD43 mucins became sialyl-Lewis a reactive, but after different times as revealed by pulse-chase studies. However, only MUC1 became sialyl-Lewis x reactive. These differences suggest that MUC1 and CD43 are synthesized in different compartments of the cell. It was also observed that the mucins from colon carcinoma patients had MUC1-type mucins that carried both sialyl-Lewis a and x epitopes and CD43-type sialyl-Lewis a mucins with only low levels of sialyl-Lewis x epitopes. One could hypothesize that colon carcinoma derived MUC1 is decorated with potent E-selectin epitopes, and that this could be one of several reasons for the involvement of MUC1 in cancer development.

Adenocarcinoma↗