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Biomedical subjects

R A Wild

Publications and source records attributed to R A Wild.

64 records · Page 4Linked to original sources
PubMed1970

Oestrus and gasric secretion in the dog.

Marked decreases in 24-hour Heidenhain pouch secretion, lasting from nine to 18 days, were observed in five mongrel bitches during periods of naturally occurring oestrus. Neither oophorectomy nor control celiotomy caused significant change in Heidenhain pouch secretion in bitches who were not experiencing oestrus. The oral administration of diethyl stilboestrol, in doses ranging between 5 mg and 70 mg per day, had no clear-cut effect on Heidenhain pouch secretion.

Abdomen↗
PubMedDate not supplied

Whole endometrial fragments form characteristics of in vivo endometriosis in a mesothelial cell co-culture system: an in vitro model for the study of the histogenesis of endometriosis.

OBJECTIVE: We have previously reported on the effects of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) on the adhesion of human endometrial stromal cells to peritoneal mesothelial cells in vitro. The relevance of this co-culture system to in vivo endometriosis remains to be established. We contrasted endometrial fragments with endometrial stromal cells to determine their relevance. METHODS: Human mesothelial cells were isolated from peritoneal fluid from four normal women via Ficoll-Paque gradient centrifugation at 400 x g for 30 minutes and grown in M199 medium containing epidermal growth factor (10 ng/mL) and 10% fetal calf serum. The homogeneous cells were cultured on collagen-coated plates until a monolayer formed. Endometrial stromal cells or whole endometrial fragments, isolated from endometrial tissue of four normal women, were put on the mesothelial monolayer and cultured at 37 degrees C for 24 days. After fixation, the samples were incubated with monoclonal antibody to epithelial membrane antigen, cytokeratin, and vimentin, and processed with standard immunohistochemical techniques. RESULTS: Observation under phase contrast microscopy revealed that, in this co-culture system, whole endometrial fragments demonstrated characteristic morphology of in vivo endometriosis, whereas isolated endometrial stromal cells did not. CONCLUSION: Whole endometrial fragments, but not isolated endometrial stromal cells, form morphologically characteristic structures similar to in vivo endometriosis in this co-culture system. It is hoped that this system might be useful for studying certain aspects of the histogenesis of endometriosis.

Adult↗
PubMedDate not supplied

The gynecologist and cardiovascular disease: a window of opportunity for prevention.

OBJECTIVE: We present current concepts and assess the quality of information available for the prevention of cardiovascular disease in women. METHODS: This article reviews research bearing on the prevention of cardiovascular disease in women, with particular attention to modifiable risk factors. We describe the magnitude of the problem and assess the quality of the data with respect to the classic risk factors. The concept is emphasized that changes at menopause, states of endocrine aberration, and benefits and risks of hormone substitution and oral contraception must be understood in conjunction with all other potentially modifiable and nonmodifiable risk factors. CONCLUSIONS: Primary care physicians, especially obstetrician/gynecologists, have a pivotal role to play in the reduction of this disease. Behavior modification is the key to integrating prevention into the regular annual visit.

Cardiovascular Diseases↗
PubMedDate not supplied

Feasibility and implementation of a hospital-based group practice.

All sectors of the economy have been suffering from the current inflationary environment. In some businesses, such as health care, costs have increased more than thought possible in the past. Since hospitals are labor intensive, they have been drastically affected by the push toward higher pay and the often hidden cost of fringe benefits. It is not surprising, therefore, that in seeking relief from the cost, hospitals will examine a method which removes a large part of the salary burden from their budgets: conversion of hospital-based salaried physicians to private, fee-for-service groups. The first article in this two-part series, which appeared in Medical Group Management, November/December 1981, examined the financial and legal aspects of the conversion. This concluding article presents the operational and organizational issues which must be considered for a successful conversion.

Appointments and Schedules↗