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Biomedical subjects

R A Ormsbee

Publications and source records attributed to R A Ormsbee.

34 records · Page 2Linked to original sources
PubMed1968

Passive cutaneous anaphylaxis with antigens from Coxiella burneti.

Passive cutaneous anaphylaxis (PCA) was produced in guinea pigs sensitized with guinea pig Coxiella burneti phase I-II antiserum and challenged with dimethylsulfoxide- or trichloroacetic acid-soluble extracts from phase I cells. The PCA reaction could not be induced by whole or mechanically disrupted phase I or phase II C. burneti cells or by extracted cells or extracts of phase II cells. The antibody responsible for PCA was in the 7Sgamma(1) (fast gamma) globulin. Sensitization of the skin by 7Sgamma(1) antibody could be blocked nonspecifically by 7Sgamma(1) globulin from normal serum or from phase II antiserum. The 7Sgamma(2) (slow gamma) globulin antibody inhibited the reaction specifically. Some antiserum pools containing high agglutinin and complement-fixing titers to phase I C. burneti cells failed to initiate the PCA reaction, perhaps due to an imbalanced ratio of gamma(1) to gamma(2) specific globulins or to an imbalance in the ratio of specific to nonspecific gamma(1) globulins. Agglutinins to phase I cells were found in both gamma(1) and gamma(2) antibody globulins. Complement-fixing antibodies were found in the gamma(2) globulin fraction.

Agglutination Tests↗
PubMedDate not supplied

A comparison of some biologic characteristics of isolates of the Legionnaires' disease bacterium.

The ability of three isolates of serogroup 1 and one isolate of serogroup 4 of Legionnaires' disease bacterium (LDB) to infect and cause fever and death in guinea pigs was studied, as well as their ability to produce plaques in cultured primary chick embryo cells. The serogroup 4 isolate originally was recovered from cord clot and placental tissue from a healthy mother following delivery of a normal child. The effects on LDB of prolonged cultivation on supplemented Mueller-Hinton (MH) agar medium and of subsequent cultivation in yolk sacs of chick embryos were examined. Prolonged cultivation of LDB on MH medium resulted in great loss of ability to produce plaques and to cause fever and death in guinea pigs. Subsequent passage in embryonated eggs of MH-adapted LDB tended to restore ability to produce plaques and to cause infection and illness in guinea pigs. Fatty acid composition profiles of the four strains were similar to each other.

Animals↗