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Biomedical subjects

R A Lopes

Publications and source records attributed to R A Lopes.

At least 19 recordsLinked to original sources
PubMed2003

Electromyographic evaluation of the adductor pollicis muscle of elderly patients carriers of removable partial prosthesis when using acrylic devices adapted to the handle of toothbrush.

The aim of this study was to evaluate the muscular activity of the adductor pollicis muscle, by electromyography, when using a device made of acrylic resin adapted to the handle of toothbrush. 10 patients of both sexes from the Clinic of Removable Partial Dentures of the Dental School--USP, with a mean age of 65 years, were selected. Electromyographic records were obtained with patients in clinical conditions of Initial Rest and under conditions of claw apprehension and dental brushing. The mean results obtained, in mV, were: sat men: 2.97 for rest (R); 11.55 for free closure (FC); 35.80 for strained closure (SC); 29.30 for simulated brushing (SB); 55.00 for brushing (B); 26.87 for simulated brushing with the device (SBA); 69.70 for brushing with the device (BA); 3.72 for post exercise rest (PR). Standing: 2.27 for rest (R); 13.92 for free closure (FC); 45.85 for strained closure (SC); 28.40 for simulated brushing (SB); 72.07 for brushing with the device (BA); 1.42 for postexercise rest (PR). Sat women: 9.78 for rest (R); 22.71 for free closure (FC); 41.48 for strained closure (SC); 26.45 for simulated brushing (SB); 52.78 for brushing (B); 17.00 for simulated brushing with the adapter (SBA); 42.38 for brushing with the adapter (BA); 14.40 for postexercise rest (PR). Standing: 5.26 for rest (R); 17.81 for free closure (FC); 46.55 for strained closure (SC); 40.61 for simulated brushing (SB); 61.71 for brushing (B); 26.08 for simulated brushing with the adapter (SBA); 54.06 for brushing with the adapter (BA); 3.46 for postexercise rest (PR). The adductor pollicis muscle showed a higher electromyographic activity, considered statistically similar to each other, in 3 clinical conditions analyzed: brushing (B), brushing with the adapter (BA) and strained closure (SC). The adductor pollicis muscle showed lower electromyographic activity, considered statistically similar, in 5 clinical conditions analyzed: simulated brushing (SB), simulated brushing with the adapter (SBA), free closure (FC), rest (R) and postexercise rest (PR).

Acrylic Resins↗
PubMed2003

EMG analysis of the upper and lower fascicles of the orbicularis oris muscle in deaf individuals.

The aim of this study was to analyze electromyographically the upper and lower fascicles of the orbicularis oris muscle in deaf patients comparing them with clinically normal patients. These patients present several disorders in facial muscles, temporomandibular joints disorders and constantly headache in virtue of not the use of the face expression muscles. This was performed in 30 patients from both sexes with a mean age of 18.5 years, divided into 3 groups of 10 patients each: 1. deaf patients with buccal breathing and incompetent lips; 2. in deaf patients with nasal breathing and competent lips; 3. clinically normal patients, in 7 clinical conditions: at rest with and without labial contact, sucking water, blowing, pursing and reciprocal compression of the lips and the emission of the syllabi "PA". It was observed that in the deaf patients group, both fascicles of the orbiculares oris muscles presented hyperactivity in most of the analyzed movements, mainly in buccal breathing with incompetent lips, and the lower fascicle demonstrated the highest electromyographic activity.

Adolescent↗
PubMed2001

Modification of col shape and interproximal area after periodontal surgery associated with the restorative alveolar interface (RAI) technique. Histological study in dogs.

The modification of the col shape and position by the restorative alveolar interface technique (RAI) was studied in the interproximal areas between the mandibular first molars and fourth premolars of 10 dogs. Full thickness flaps were raised to expose the interproximal root surface and alveolar bone crest. The RAI procedure was performed only on the experimental sides and the control areas were the opposite side of the same animal. The animals were sacrificed at zero hour, 7, 14, 21 and 28 days for histological analyses. Approximately 6.0-micron-thick sections were made in buccolingual and mesiodistal directions and stained with hematoxylin-eosin and Mallory for light microscopy analysis. A satisfactory healing process was observed up to the 14th and 21st days which showed a modified col shape. At this time, an inflammatory reaction developed affecting the evolution of the healing. The surgery had probably created conditions for the installation of an inflammatory process resulting from the modified anatomy of the interdental area.

Alveolar Process↗
PubMed2000

Identification of a 30 kDa antigen from leishmania (L.) chagasi amastigotes implicated in protective cellular reponses in a murine model.

An antigen of apparent molecular mass of 30 kDa, termed p30, was purified from Leishmania (L.) chagasi amastigotes after separation of parasite extracts by sodium dodecyl sulfate-polyacrylamide gel eletroctrophoresis followed by electroelution. The use of the purified antigen in lymphocyte cultures from BALB/c mice previously immunised with L. (L.) chagasi amastigotes led to high levels of proliferation. Animal immunisation with p30 plus complete Freund's adjuvant either by subcutaneous or intraperitoneal route led to comparable antigenic stimulation. Similar stimulation indices induced by p30 were also obtained when animals were immunised with Corynebacterium parvum as adjuvant by the intraperitoneal route. Detection of IL-2 and IFN-gamma in the supernatants from lymphocytes stimulated by p30 and inhibition of the production of these lymphokines in the presence of anti-CD4 strongly indicated the involvement of the Th1 subset in the responses elicited by p30 antigen. Immunisation of BALB/c mice with p30 provided partial protection against challenge with L. (L.) chagasi amastigotes, indicating a protective role for p30 and that Th1 can be related to accquired resistance to visceral leishmaniasis in a murine model. Further characterisation studies were performed by the use of a monoclonal antibody directed to a cysteine proteinase of 30 kDa from L. (L.) amazonensis amastigotes. Despite the cross-reactivity presented by p30 from both Leishmania species, the p30 from L. (L.) chagasi amastigotes lacks proteolytic activity.

Animals↗
PubMed2000

Latex allergy in health care personnel.

This article presents a summary of literature published in recent years about latex allergy. The first article on allergic reactions associated with latex was published in Britain in 1979. Since then, the number of reported cases has increased. A wide variety of allergic reactions that range from contact dermatitis to anaphylactic shock are described in literature. Conclusions support that health care team members must be well informed about the implications of latex allergy to better help allergic individuals.

Adult↗
PubMed1999

Angioarchitectural arrangements in corrosion cast of the nasal septum of the Mongolian gerbil.

The three-dimensional architecture of the vascular network of the nasal septum of the gerbil rat was studied using the corrosive resin cast technique. The angioarchitecture of the nasal septum observed with scanning electron microscopy (SEM) showed that a vascular network is disposed in the lamina propria. The capillaries, arterioles, venules and arteries were closely observed in different areas of the nasal septum mucosa.

Animals↗
PubMed1999

Scanning electron microscopy of the rat tongue mucosa with special attention to the bacteria on epithelial cell membranes.

The authors examined the filiform and fungiform papillae surfaces of rat tongue by scanning electron microscopy showing the numerous groupings of bacteria on the epithelial cell membranes. The fungiform papillae were round in shape and present few bacteria. The epithelial cell of filiform papillae revealed numerous streptococci. The grouping of the bacteria are attached on the epithelial cell membrane, demonstrating three-dimensional SEM images.

Animals↗
PubMed1997

Biochemical and histopathological alterations induced in rats by Tityus serrulatus scorpion venom and its major neurotoxin tityustoxin-I.

Intravenous injection into the rat of sublethal doses of Tityus serrulatus scorpion venom (100 micrograms protein/kg) or its major neurotoxin tityustoxin-I (TsTX-I, 20 micrograms/kg) caused, 30-180 min after injection, statistically significant increases in the serum levels of aspartate aminotransferase, amylase, creatine kinase and lactate dehydrogenase, as well as hyperglycemia, a high level of plasma free fatty acids and a low level of liver glycogen. The in vitro serum levels of the above enzymes did not change. For alanine aminotransferase, gamma-glutamyl transferase and alkaline phosphatase, neither in vitro nor in vivo alterations were observed. The whole venom and TsTX-I caused hepatic congestion with hemolysis and hydropic degeneration. Other histological lesions included edema and congestion with subpleural hemorrhage in the lungs, hypertrophy of fibers with degeneration areas in the heart, and congestion and hemorrhage in the kidneys. In the salivary glands, alterations to the acini and ductules were visible. In the adrenal glands no morphological alterations could be detected at the studied doses. The results suggest that the in vivo enzymatic and histopathological alterations are due to tissue lesions evoked by the whole venom and TsTX-I. An indirect effect, however, induced by stimulation of acetylcholine and catecholamine release in the postganglionic nerve terminals, cannot be excluded.

Animals↗
PubMed1997

Scanning electron microscopy study of the interface epithelium-connective tissue surface of the lingual mucosa in Calomys callosus.

The characteristics of the interface epithelium-connective tissue of the lingual mucosa of Calomys callosus was studied by employing scanning electron microscopy after removal of the epithelial cells. In the anterior part, the connective tissue papillae of filiform papillae are distributed at random in their original configuration, and the round-shaped fungiform papillae are scattered among the filiform papillae. In the middle part, the filiform papillae present several rod-shaped projections. In the posterior part, the vallate papilla are oval in shape, and the connective tissue reveals a large ridge separated by deep lateral grooves. The posterior adjacent area is a flat surface and shows several openings of salivary gland ducts. The foliate papillae are formed by several small ridges separated by grooves. In the areas adjacent to the foliate papilla, small pointed and laminar papillae were observed.

Animals↗
PubMed1997

The annual testicular cycle of the domestic quail (Coturnix coturnix japonica).

A morphometric study was conducted on the testis of the domestic quail Coturnix coturnix japonica to determine testicular kinetics. We investigated the variability along the year of testicular parameters such as seminiferous tubule diameter, germinal epithelium height and amount of meiotic figures of maturing spermatids in the seminiferous epithelium and of sperm in the tubular lumen. The results of morphometric analysis showed the occurrence of an annual testicular cycle defined by four distinct phases: a resting phase (at the end of summer), a recrudescence phase (in the fall), a proliferative phase (at the end of winter and beginning of spring), and a regression phase (spring and summer). We also observed that the testes of adult quails present elevated and maximal spermatogenic activity in fall-winter (short-day period) and at the beginning of spring, respectively, and lower values in spring and summer (long-day periods), with minimum values at the end of summer.

Animals↗
PubMed1997

[The most frequent nursing diagnoses in an oncology admission unit].

The aim of this paper was to survey the most frequent nursing diagnoses by means of the nursing history and medical procedure in order to help the care provided at the Oncology Nursing Service of Women Health Whole Care (Centro de Atenção Integral à Saúde da Mulher-CAISM). The five most common diagnoses were: risk for infection, risk for altered body temperature, risk for aspiration and colonic constipation of the exchanging pattern, and risk for activity intolerance of the moving pattern. Data collection was based on these patterns. Data predominantly involving social, cultural, psychological and spiritual aspects were hardly interpreted, because they were poorly registered or omitted.

Adolescent↗
PubMed1996

Effect of CO2 laser on Class V cavities of human molar teeth under a scanning electron microscope.

The purpose of this study was to evaluate the effects of CO2 laser on dentin of class V cavities of extracted human molar teeth using a scanning electron microscope. SEM showed a smooth area with concentric lines formed by melting with subsequent recrystallization of dentin, areas of granulation, vitrified surface, numerous cracks, and irregular areas of descamative dentin. These data indicate that CO2 laser (4 and 6 watts) produces dentin alterations and limit its clinical applications.

Carbon Dioxide↗
PubMed1995

Effect of acyclovir on rat fetus palate mucosa.

Five pregnant rats were treated during organogenesis with sc injections of acyclovir (50 mg/kg body weight) on days 9, 10 and 11 of pregnancy. The fetuses (N=62) were evaluated on day 20 of gestation and presented decreased body weight as well as delayed differentiation of fetal rat palate epithelium, with increased nuclear volume, decreased cytoplasmic and cellular volumes, decreased epithelial and keratin thicknesses, and increased cellular numerical density.

Abnormalities, Drug-Induced↗
PubMed1994

The male reproductive organs in experimental Chagas' disease. III. Plasma testosterone and accessory sex glands in the acute phase of the disease.

Male Wistar rats were injected i.p. with 2 x 10(5) trypomastigotes of the Bolivia strain. Fifteen days later, few parasite nests were observed in the fibromuscular layer surrounding the seminal vesicle acini of chagasic animals and no parasites were detected in the testis and ventral prostate. A significant decrease was observed in the absolute weight and fructose content of the seminal vesicle and ventral prostate of chagasic rats, which also presented a sharp decrease in plasma testosterone levels.

Animals↗
PubMed1994

Effect of hyperthermia on rat fetus palate epithelium.

Maternal hyperthermia during gestation induces delayed cellular growth and differentiation in fetal rat palate epithelium, with increased nuclear, cytoplasmic and cellular volumes, increased epithelial thickness, decreased keratin and cellular numerical density.

Animals↗
PubMed1993

Alcohol-induced alterations in the palate epithelium of the rat fetus.

Maternal alcoholism (ethanol and sugar cane brandy) during gestation induces delayed cellular growth and differentiation in fetal rat palate epithelium, with increased nuclear, cytoplasmic and cellular volumes, increased epithelial and keratin thickness and decreased cellular numerical density.

Animals↗
PubMed1992

The male reproductive organs in experimental Chagas' disease. II. Morphometric study of the vas deferens in the chronic phase of the disease.

A previous study showed that the vas deferens of mice in the acute phase of Chagas' disease had a slight increase in the muscle layer area associated with a small decrease in the luminal area. The vas deferens of chronic chagasic mice, investigated in the present experiment, presented a marked increase in the luminal area in addition to a significant thinning of the muscle and epithelium layers. The structural alterations of the vas deferens observed in the acute and chronic phases of Chagas' disease were compared with the evolution of chagasic esophagopathy.

Acute Disease↗