A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
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Autoradiography↗
Biomedical subjects
R A Laskey
Publications and source records attributed to R A Laskey.
The translation of viral RNAs in frog oocytes.
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Animals↗
Induction of polyoma DNA synthesis by injection into frog-egg cytoplasm.
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Animals↗
Inhibition of ribosomal RNA synthesis in neurula cells by extracts from blastulae of Xenopus laevis.
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Animals↗
Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis.
RNA from encephalomyocarditis virus was injected into oocytes of Xenopus laevis. After incubation of the oocytes in [(35)S]methionine, polyacrylamide gel electrophoresis showed that a new series of polypeptides had been synthesized. They were identical in size to the polypeptides that appeared in ascites cells after infection with this virus. Electrophoretic and chromatographic analysis of the methionine-containing tryptic peptides from three of the induced polypeptides confirmed that they were virus-specific. All of the bands that appeared in ascites cells after infection also appeared in oocytes after injection of RNA from the virus. We conclude that Xenopus oocytes translate a mammalian viral mRNA faithfully and extensively, and perform normal post-translational modifications.
Animals↗
Genetic content of adult somatic cells tested by nuclear transplantation from cultured cells.
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Animals↗
The use of antibiotics in the preparation of amphibian cell cultures from highly contaminated material.
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Animals↗
Methods of transplanting nuclei from single cultured cells to unfertilized frogs' eggs.
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Animals↗
The transplantation of nuclei from single cultured cells into enucleate frogs' eggs.
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Animals↗
Nuclear import: a tale of two sites.
The recently determined crystal structure of a nuclear localization sequence receptor has revealed an exquisitely specific interaction between ligand and receptor, and explains how simple and complex nuclear localization signals can both be recognized specifically by the same molecule.
Animals↗