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Biomedical subjects

R A Good

Publications and source records attributed to R A Good.

At least 631 records · Page 35Linked to original sources
PubMed1978

Successful transplantation of marrow from an HLA-A, -B, -D mismatched heterozygous sibling donor into an HLA-D-homozygous patient with aplastic anemia.

A patient with aplastic anemia who was found to be homozygous for an HLA-D determinant shared by her unrelated parents achieved sustained engraftment and full restoration of hematopoietic and lymphoid function following a transplant from an HLA-A and -B nonidentical, ABO incompatible sibling who was heterozygous for the shared HLA-D specificity. Transplantation was complicated by transient graft-versus-host disease of moderate severity, which resolved completely following treatment with antithymocyte globulin and prednisone. The case indicates that patients found to be HLA-D-homozygous may be successfully transplanted from HLA-D-heterozygous sibling donors despite HLA-A and HLA-B incompatibilities, and thus further demonstrates the importance of the HLA-D region as a marker of donor-host histocompatibility.

Anemia, Aplastic↗
PubMed1978

In vitro responses of peripheral blood and spleen lymphoid cells to mitogens and antigens in childhood Hodgkin's disease.

Over the past 5 years, parameters of immunological function have been determined in all children with biopsy-proven Hodgkin's disease in the Department of Pediatrics, Memorial Sloan-Kettering Cancer Center. This report summarizes the in vitro responses of lymphocytes to stimulation by mitogens and antigens (between January 1975 and December 1976) in 33 of these previously untreated patients. In nine of these patients, responses of the splenic lymphocytes were studied concomitantly with the responses of peripheral blood lymphocytes. Peripheral blood from normal children and adults was used as the control. Our results have demonstrated no significant differences between the responses of normal children and adult controls. The absolute necessity for concomitant studies of the controls and the patients was shown. The value of examining multiple concentrations of a single mitogen was also well defined. In the children with Hodgkin's disease, there was a consistent failure of the peripheral blood lymphocytes to respond to the lower concentration dose of phytohemagglutinin. However, this abnormality was not found in the splenic lymphocytes.

Adolescent↗
PubMed1978

Transplacental lung tumorigenesis in the athymic mouse.

Female BALB/c nu/+ mice, pregnant by nu/+ males (nu: gene for hairlessness-athymia) were given injections of urethan, a transplacental tumorigen, on Day 17 or 19 of gestation. After an average of 16 weeks under clean conventional conditions, the incidence of primary lung tumors was similar in nude and normal offspring treated with carcinogen on either gestational day, with a higher incidence after treatment of Day 19. Thus, the absence of thymus did not affect the occurrence of transplacentally induced primary lung tumors or alter the well-known perinatal increase in sensitivity. Histologically, the nu/nu tumors differed from normal in the appearance of many atypical basophilic cells and in a tendency to invade both the parenchyma and the pleural surface. These results suggested progression of the lung adenomas to a more atypical, invasive form, a progression that may have occurred prematurely in the absence of thymus-dependent immune response.

Adenoma↗
PubMed1978

Dietary restrictions early and late: effects on the nephropathy of the NZB X NZW mouse.

NZB X NZW F1 mice initiated on calorie restriction at weaning or at 4 to 5 months of age or initiated on moderate protein restriction at weaning, were afforded significant protection from the development of immune nephritis. Whereas animals on normal calorie intake demonstrated deposition of immune reactants in glomerular basement membrane-oriented pattern, those on either protein or calorie restriction exhibited mesangial confinement of immunoglobulins and complement. Associated with these divergent patterns of immune deposition, mice on normal calorie intake evidence extensive cellular proliferation and glomerular sclerosis while dietary restricted mice demonstrated virtually no hyalinization and only mild cellular proliferation. Autoantibody formation of calorie-restricted animals was significantly decreased compared to mice fed a normal diet. Thus, moderate dietary restriction may serve as either a prophylactic or effective therapeutic approach to ongoing autoimmune disease.

Aging↗
PubMed1978

Rosette formation of human null lymphocytes with Rhesus monkey erythrocytes.

Human null lymphocytes which lack B- and T-lymphocyte markers were found to form spontaneous rosettes with uncoated Rhesus monkey erythrocytes. Rosette-forming null lymphocytes were determined in null lymphocyte-enriched preparations of healthy persons and of patients with lymphoproliferative diseases. Quantitative determinations of these rosette-forming lymphocytes and other cells bearing B- or T-cell markers showed that some, and not all, of the null lymphocytes possess this rosette-forming capacity. The possibility of these null lymphocytes being related to T lymphocytes, but at a different cellular stage, is discussed.

Animals↗
PubMed1977

Reconstitution in severe combined immunodeficiency by transplantation of marrow from an unrelated donor.

A patient with severe combined immunodeficiency received seven transplants of bone marrow from an HLA-B-compatible and HLA-D-compatible unrelated donor in an attempt to provide immunologic reconstitution. The first four transplants achieved restricted engraftment with evidence of rudimentary immunologic function. A fifth transplant, given after low-dose cyclophosphamide, produced reconstituion of cell-mediated immunity. Marrow aplasia developed after recontamination with a nonpathogenic microflora. Transplantation of marrow previously stored in liquid nitrogen was ineffective. A subsequent transplant, administered after high-dose cyclophosphamide, achieved durable engraftment, with complete hematopoietic and immunologic reconstitution. Seventeen months after transplantation, full functional engraftment persists. Graft-versus-host disease has been chronic and moderately severe, but limited to the skin and oral mucosa. Transplantation of marrow from unrelated histocompatible donors may provide a useful treatment for patients with severe combined immunodeficiency or aplastic anemia who lack a matched sibling or related donor.

Bone Marrow Transplantation↗
PubMed1977

Circulating thymic-hormone activity in congenital immunodeficiency.

Circulating thymic-hormone activity was assayed by measuring Thy 1-2 antigen induction on null lymphocytes from athymic mice incubated with human plasma or serum. Plasma from 19 normal children aged under 10 had inductive activity equivalent to 10-6-16-2 ng thymopoitin/ml. Plasma from 15 infants were severe combined immuno-deficiency, 2 of whom had appreciable immunoglobulin synthesis, and from 2 infants with DiGeorge syndrome had little or no inductive activity. Successful reconstitution with thymus or bone-marrow grafts and with red-cell infusions (if adenosine-deaminase deficiency is present) was followed by a rise in circulating thymic-hormone activity.

Agammaglobulinemia↗
PubMed1977

Phorbol myristate acetate: a mitogen selective for a T-lymphocyte subpopulation.

Phorbol myristate acetate (pma) is a potent mitogen for human peripheral blood lymphocytes (PBL) comparable to phytohemagglutinin (PHA) in potency. Inactivation of PHA-responsive lymphocytes by 5'-bromodeoxyuridine and light treatment left the PMA response intact and nice versa. Experiments separating lymphocytes by rosetting with sheep erythrocytes (SRBC) demonstrated that the PMA-responsive lymphocytes segregate with those that have a high affinity for SRBC to a greater than PHA- or concanavalin A (Con A)-responsive cells. These results indicate that a PMA-responsive population in human peripheral blood resides within the T-lymphocyte population and appears to have a high affinity for SRBC and to be distinct from that responding to PHA and Con A. PMA may be useful clinically to assay the size and function of the high affinity or "active" rosette population.

Animals↗
PubMed1977

Relationship of a T-lymphocyte marker to phase of cell cycle and morphology of leukemic cells.

Spontaneous rosette formation with sheep erythrocytes (SRBC) was studied in the peripheral blood and bone marrow lymphoid cells from a patient whose leukemic cells appeared to be T-lymphocytes. Simultaneous morphological examination of the peripheral blood white cells indicated that they consisted of 21% lymphoblast; 26% prolymphocytes and 48% mature lymphocytes. The distribution of bone marrow cells within the cell cycle was determined by flow microfluorometry and 7 hours after treatment with vincristine consisted of 69% in G1, 21% in S, and 9% in mitosis. Since virtually all the cells both in marrow and blood formed rosettes with SRBC this implies that the expression of this T cell marker is independent both of the morphological appearance of these cells and their position within the cell cycle.

Adult↗