Biomedical subjects

Qin-kai Deng

Publications and source records attributed to Qin-kai Deng.

7 recordsLinked to original sources

PubMed2006

Mathematical model of phosphatidylinositol-4,5-bisphosphate hydrolysis mediated by epidermal growth factor receptor generating diacylglycerol.

Phosphatidylinositol-4,5-bisphosphate (PIP2) is hydrolyzed in response to the tyrosine phosphorylation of the epidermal growth factor receptor (EGFR) and plays an important role in regulating cell proliferation and differentiation through the generation of second messengers diacylglycerol (DAG) and trisphosphate inositol (IP3) which lead to the activation of protein kinase C (PKC) and increased levels of intracellular calcium, respectively. In the paper, a mathematical model was established to simulate the accumulation of DAG due to PIP2 hydrolysis mediated by EGFR. Molecular mechanisms between DAG, PIP2, EGFR and phosphatidylinositol transfer protein (PITP) were explained successfully, and positive cooperativity which existed between phospholipase C-gamma1 (PLC-gamma1) and PIP2 was also explained. In the model the effects of parameters on simulation of PIP2 hydrolysis were analyzed and the efficacies of some molecular intervention strategies were predicted. To test the coherence between the model and the biological response to epidermal growth factor (EGF) in cells, the levels of DAG and the tyrosine phosphorylation-EGFRs in NIH3T3 mouse embryonic fibroblast (MEF) were determined by biochemical experiments which showed that the accumulation of DAG was a sigmoidal function of phosphorylation-EGFR concentration, and the consistency between the mathematical model and experimental results was confirmed. In brief, this mathematical model provided a new idea for the further study of the dynamic change of biological characteristics in inositol phospholipid hydrolysis, predicting the efficacy of molecular intervention and the relationship between the metabolisms of inositol phospholipid and other signal transduction pathways.

Animals↗

PubMed2006

[A novel model of the medical instrumentation--an overview of wearable sensors and systems].

The traditional medical instrumentation is now not suited for the transformation of the present healthcare model. It's an attempt to make a breakthrough on traditions to develop wearable sensors/systems. Five articles about wearable technology are selected by this special topic. And our aim is to inspire the designers of medical devices to grasp the chance and to take a vigorous action on innovatory developments, so as to satisfy the huge requirements of the healthcare market, and to open a new era of the medical device industry.

Clothing↗

PubMed2004

[Mathematical model of epidermal growth factor receptor-mediated lipid phosphatidylinositol(4,5)-bisphosphate hydrolyzation by phospholipase C-gamma1 activity].

OBJECTIVE: To investigate the dynamic characteristics of lipid phosphatidylinositol (4,5)-bisphosphate (PIP(2)) in plasma membrane hydrolyzed by phospholipase C-gamma1 in epidermal growth factor receptor(EGFR)-mediated signal pathway. METHODS: A mathematical model based on the law of mass action was established with differential equations to simulate metabolizable pathway of PIP(2). RESULTS: Differential equations of the key product concentration during hydrolysis of PIP(2) were formulated, and the effects of the parameters on these hydrolyzed products analyzed. CONCLUSION: This mathematical model provides foundation for further investigation of the dynamic changes of biological characteristics and the relations between the key product concentrations in PIP2 hydrolysis.

ErbB Receptors↗

PubMed2004

[Value of galvanic skin response in assessment of diabetic autonomic neuropathy].

OBJECTIVE: To explore a method for early detection of diabetic autonomic neuropathy (DAN) on the basis of analysis of galvanic skin response (GSR). METHODS: In 25 randomly selected diabetic inpatients (14 with clinical DAN symptoms), GSR signals were collected from the fingers of both hands based on Virtual Instrumental WorkBench-LabVIEW and compared with those acquired from normal subjects. RESULTS: The GSR indices of patients either with or without DAN were different from those of normal subjects, and even in the same patients, the changes of these indices after clinical treatment were also obvious. CONCLUSION: GSR detection and analysis can help understand the impairment of the autonomic system and facilitate DAN assessment.

Aged↗

PubMed2003

[A method for transfering physiological data through GSM wireless communication network].

This article describes a method for transfering physiological data through GSM wireless communication network. The data of heart rate and body temperature are collected by a microcontroller and transmitted through serial ports to the GSM data module, which then transmits the data to a remote terminal through GSM network. This method ensures cheap and stable long-distance data transfer within the coverage of the GSM network.

Body Temperature↗

PubMed2003

[Nonlinear dynamic analysis of heart rate variability in patients with diabetic autonomic neuropathy].

OBJECTIVE: To explore earlier detection methods for diabetic autonomic neuropathy (DAN) by heart rate variability (HRV) analysis. METHODS: Thirty-four diabetic patients (including 22 with explicit clinical DAN symptoms) were randomly selected for this study from the in-patient department of endocrinology. On the basis of Virtual Instrumental WorkBench- LabVIEW and using several nonlinear dynamic analysis measures, including Allan factor, lyapunov exponent, approximate entropy, fractal dimension, complexity, wavelet-transform standard deviation and nonlinear energy operator, the analysis of the HRV in these diabetic patients was performed in comparison with normal subjects. RESULTS: The nonlinear indices of both DAN patients and patients without obvious DAN were significantly different from those of the normal subjects, especially in terms of Lyapunov exponent, approximate entropy, and nonlinear energy operator. CONCLUSION: Nonlinear dynamic methods of HRV analysis can provide assistance in assessing the status and impairment of the autonomic system, and can be used to efficiently detect diabetic neuropathy in early stages.

Autonomic Nervous System Diseases↗

PubMed2003

[A new approach for exploring the essence of meridian].

The paper includes a brief review and evaluation of the progress in the research of the essence of meridian in China in the last two decades, in which the author suggests that more effort at all levels involving multiple disciplines needs to be investigated in the pursuit of this issue after the failure of prolonged research in the fields of histological anatomy. Microcosmic investigation, in particular, should be pursued making full use of the available achievement in molecular cell biology, genome research, biophysics and biochemistry and so forth, so as to build a solid plateform for making a breakthrough. The author urges the importance of research in the information carrier that regulates the meridian physiological network of the body. Based on the theories in cellular signal transduction and relevant experimental data, the author points out that the signal amplitude and frequency of intracellular Ca(2+) oscillations and intercellular Ca(2) wave elicited by specific acupuncture may contain information of the meridian routes and physiological function regulations. Through its binding proteins, Ca(2+) participates in almost all the physiological processes such as reproduction, metabolism, muscle contraction to cognition and memory. It is therefore hypothetically believed that Ca(2+) and inositol trisphosphate (IP(3), which is required for the propagation of intercellular Ca(2+) waves) can be the long-quested information carriers of the meridian physiological network, and they in conjunction with the gap-junction protein (connexon) build the material bases of the meridian. The author also specifies some possible directions of further study and experimental methods.

Calcium Signaling↗