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Biomedical subjects

Q Zhu

Publications and source records attributed to Q Zhu.

At least 289 records · Page 16Linked to original sources
PubMed1992

Large-transducer measurements of wavefront distortion in the female breast.

Ultrasonic waves propagating through soft tissue experience wavefront distortion. Refraction occurs at boundaries between tissue beds having different sound speeds; scattering occurs within a tissue bed, caused by local impedance variations. This paper describes measurements of wavefront distortion in the human female breast that indicate that refraction is the dominant distortion mechanism when the ultrasonic phased array is very large. The determination that refraction dominates the wavefront distortion is based upon studies of multiple image artifacts that result from a single source radiated through in vivo breasts and breast phantoms. The receiving apertures used were 4.65 and 9.6 cm. Such image artifacts are repeatedly observed in the 10 young subjects reported in this paper, and also in older subjects. An understanding of the in vivo observations is obtained by phantom studies.

Adult↗
PubMed1992

Isolation and mode of action of rabbit corticostatic (antiadrenocorticotropin) peptides.

Corticostatic peptides are a family of arginine-rich cysteine-rich peptides that inhibit ACTH-stimulated corticosterone (B) production in rat adrenal cell suspensions. In this communication we describe a new method for the facile isolation and purification of these basic peptides from rabbit adult lung. We then describe the isolation and sequences of the four rabbit peptides, CSI, CSII, CSIII, and CSIV, and compare their biological activities in the ACTH (150 pg/ml) inhibition assay. CSI is by far the most potent of the four peptides. Using CSI as a model, we then studied its effects on the proximal and distal parts of the pathway leading to the generation of cAMP. CSI had no effect on (Bu)2cAMP action on forskolin or cholera toxin in their ability to mimic ACTH and increase B production in rat adrenal cells, nor did CSI have any effect on the stimulation of B production by pertussis toxin. Endogenous cAMP stimulated by ACTH decreased after the addition of CSI, which pointed to the inhibition of ACTH binding to explain the mode of action of this corticostatin. Displacement of the specific binding of labeled ACTH by CSI and the ACTH antagonist ACTH-(6-24) was determined, and indeed, CSI did displace ACTH from its binding site. The question of what portion of the ACTH molecule was involved in the action of CSI was answered by studying ACTH-(1-13) acetyl amide (alpha MSH) and ACTH-(1-18) amide. CSI had no effect on alpha MSH stimulation of B production, but did lower the production of B stimulated by ACTH-(1-18) amide. Therefore, CSI must act on ACTH-(14-18), which is part of the so-called address region of ACTH, which is -Gly14-Lys15-Lys16-Arg17-Arg18-, the very basic part of the molecule. These results indicate that CSI acts by competing with ACTH for its binding receptor on the adrenal cell and that this competition is confined to amino acids 14-18 of the molecule when it is bound to the receptor.

Adrenal Glands↗
PubMed1992

Morphologic changes in basal cells during repair of tracheal epithelium.

Basal cells are differentiated with respect to junctional adhesion mechanisms and play a role in attachment of columnar epithelium to the basal lamina. Although much is known about nonciliated and ciliated cell differentiation during the repair process after injury, little is known about the basal cell. We studied the morphology of basal cells and quantitated junctional adhesion structures during repair of tracheal epithelium exposed to toxic cotton smoke. Ten adult ewes were given a smoke injury to a portion of the upper cervical trachea and were killed at 4, 6, 8, 10, and 18 days after injury for morphometric studies. At 4 days, there was a stratified reparative epithelium over the basal lamina, which was two to four cells in depth. The basal cells were identified by their hemidesmosome (HD) attachment to the basal lamina. Basal cells were about 69% larger than controls and flattened rather than columnar. The amount of HD attachment was 192% greater than controls. In contrast, volume density of cytokeratin filaments had decreased about 47%. Basal cells had returned to normal numbers and size and a columnar shape by day 18. The amount of desmosome (D) and HD attachment and volume density of cytokeratins had also reached control levels by day 18. These data indicate that morphology of basal cells changes during the initial stages of reparative regeneration but returns to normal by 18 days. Morphologic changes appear to reflect changes in size of the cell associated with cell division rather than differentiation of recently divided basal cells.

Animals↗
PubMed1991

Isolation and characterization of a rice gene encoding a basic chitinase.

Chitinase, which catalyzes the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible defenses of plants. A basic chitinase genomic sequence was isolated from a rice (Oryza sativa L.) genomic library using a bean chitinase gene fragment as a probe. The complete nucleotide sequence of the rice chitinase RCH10 gene was determined, and shown to contain an open reading frame with no introns, encoding a polypeptide of 336 amino acids. This polypeptide consists of a 21 amino acid signal peptide, a hevein domain, and a chitinase catalytic domain. The RCH10 gene has 63% identity at the nucleotide level and 75% identity at the amino acid level with chitinase genes from dicotyledonous plants such as bean, potato, and tobacco. A gene fusion of trpE and the coding region of RCH10 expressed in Escherichia coli gave a product that reacted with antiserum to bean chitinase, confirming the identity of RCH10 as a rice chitinase gene. Primer extension analysis identified two transcription start sites 53 bp and 55 bp upstream from the translation initiation codon. The 5' flanking region contains TATA and CAAT boxes, and the 3' region contains an AATAA polyadenylation signal. Southern blot hybridization indicated that there is a family of chitinase genes in the rice genome. Northern blot analysis showed that the RCH10 chitinase gene is induced in suspension cultured cells by a fungal cell wall elicitor. Rice chitinase transcripts accumulate to a high level in roots, but only low levels are found in stem and leaf tissue.

Amino Acid Sequence↗
PubMed1991

Corticostatic peptides.

In the last four years corticostatic (anti-ACTH) peptides have been isolated from human, rabbit, guinea pig and rat tissues. These peptides do not act via the cAMP cell signalling system but rather via the inhibition of the binding of ACTH to its receptor most probably through direct competition with the 14-18 sequence of ACTH for receptor binding. ACTH has specific high affinity receptors on adrenal cells but rabbit corticostatin I (CSI) has high capacity, low affinity receptors which are competed for by unlabelled excess CSI but not by excess ACTH. This indicates the presence of specific CSI adrenal cell receptors. The rabbit pituitary, hypothalamus, thalamus, adrenals, lungs and placenta contain sizeable amounts of immunoassayable CSI. Immunochemical localization of CSI indicates that it is present in the large macrophages and in neutrophils in rabbit lung, in macrophages and "supporting" endothelial cells in the spleen and in the adrenals in the cells of the zona reticularis. We have also isolated and identified new peptides which contain 12 cysteines from immune cells of humans, rats and a teleost, the carp. The functions of these peptides are now being determined. This large family of peptides may have many other, yet unidentified functions but at present we can only describe a small number of these.

Adrenal Glands↗
PubMed1991

Maternal-fetal transmission of SIV in macaques: disseminated adenovirus infection in an offspring with congenital SIV infection.

To develop a nonhuman primate model for maternal-fetal transmission of HIV infection, we have inoculated pregnant Macaca nemestrina with uncloned SIVMne. Three animals inoculated during the third trimester delivered healthy infants. One of the three infants, a male born 31 days after the mother was inoculated with SIV, became virus-positive but failed to produce SIV-specific antibody and died with overt simian immunodeficiency and disseminated adenovirus (SV20) infection at age six and one-half months. SIV and adenovirus antigen could be demonstrated by immunohistochemical methods in multiple organ systems.

Adenoviridae Infections↗
PubMed1991

The role of oxygen free radicals in myocardial ischemia/reperfusion injury.

A series of experiments have been done to investigate the role of oxygen free radicals in ischemia/reperfusion injury. The following results were found: Myocardial MDA content increased significantly after post-ischemic reperfusion in vivo and in vitro. A blockade of the xanthine oxidase pathway for free radical generation could provide effective protection against ischemia/reperfusion injury. Exogenous reactive oxygen intermediates H2O2, .OH and O2- could induce changes in the contractility and electrophysiological properties of myocardial cells similar to those seen in ischemia/reperfusion. An outburst of free radical generation was detected by ESR spectroscopy at low temperature (-173 degrees C) and with the spin trapping technique during the very early phase of reperfusion. The authors emphasize the important role of free radicals in the pathogenesis of myocardial ischemia/reperfusion injury.

Action Potentials↗
PubMed1991

[An approach to removing stains and remineralization of dental fluorosis].

341 mottled teeth were bleached with 4% and 18% hydrochloric acid; the mixture of 36% hydrochloric acid, 30% hydrogen peroxide and anesthetic ether; and 30% hydrogen peroxide warmed by infra-red ray lamp. The effects were evaluated clinically. The extracted mottled and normal teeth were treated with the methods mentioned above. The decolouration effects, the structural changes of tooth surface, and the remineralization were examined by scanning electronmicroscopy and polaroid microscopy. The results showed: all of the four methods had the decolouration effects; the decolouration effect was better in the teeth with higher mineralization and the pigmentation existed more superficially; complete decolouration was 94.8% and lightened cases were 5.2%. The mechanism of decolouration seemed to be the dissolution of tooth superficial layer containing pigment. The decalcified tooth surface might be recalcified by the treatment of recalcification solution, or by saliva itself.

Adolescent↗
PubMed1991

[Modifications of sialylation in BHK 21/C13 cells after in vitro transfection by c-Ha-ras human oncogene].

A Hamster kidney fibroblast cell line (BHK 21/C13) has been transfected by c-Ha-ras human oncogene. The expression of the oncogene significantly modified the global sialytransferase activity of the cell extract. This activity is enhanced on an average 2.5 fold whatever the acceptor. In addition, the proportion of cell-surface associated N-glycolylneuraminic acid is enhanced in transfectants, the ratio N-acetylneuraminic acid/N-glycolylneuraminic acid decreases from 7.10 to 2.80. These results suggest that a tight relationship exists, in c-Ha-ras transfected BHK cells, between the expression of the oncogene and the neuraminic acid metabolism as well as a gene regulation of glycoconjugate sialylation.

Animals↗
PubMed1990

[Short-term curative effect of cultured Cordyceps sinensis (Berk.) Sacc. Mycelia in chronic hepatitis B].

33 cases of chronic hepatitis B. patients treated with cultured Cordyceps sinensis mycelia have shown that the drug improves the liver function, promotes negative transfer HBsAg, and markedly helps to raise plasma albumin, resist high gamma globulin and to adjust body immunocompetence. It is therefore suggested that cultured Cordyceps sinensis mycelia may be used as a medicine for chronic hepatitis B. patients in adjusting protein metabolism and correcting inversion of albumin and globulin.

Albumins↗
PubMed1990

Diagnostic contrast echocardiography in children with congenital heart disease.

905 diagnostic contrast echocardiographic studies (DCE) were performed on 223 children. Carbon dioxide microbubble was used as contrast agent in various age groups, especially in newborns and infants because of its safety in application, stable contrast effect, and peripheral vein injection. Two-dimensional echocardiography (2-DCE), a useful tool in diagnosis of left to right shunt lesions reduced false negative diagnoses for ventricular septal defects and eliminated false positive diagnosis for atrial septal defects and patent ductus arteriosus. The accuracy of diagnosis in this series was 97%. The results of this series showed that 2-DCE is also valuable in the diagnosis of congenital heart disease with right to left shunts, and can be used to evaluate shunt levels and determine the obstructive sites on the right side of the heart.

Adolescent↗
PubMed1990

[Adenosine deaminase inhibitor (EHNA) on ischemia-reperfusion injury in isolated perfused rat hearts].

The effects of Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) on Langendorff perfused rat hearts subjected to ischemia-reperfusion injury were studied. Results showed that EHNA can inhibit the increase of cardiac resting tension during ischemia period and decreasing the incidence of ventricular fibrillation and its duration. The contraction amplitude, resting tension and heart rate could be recovered to preischemic level, and the coronary flow even greater than before. The authors thought that EHNA can block the breakdown of adenosine to inosine and hypothanxine, and, therefore, cut off the pathway and production of oxygen free radicals during ischemia-reperfusion injury.

Adenine↗
PubMed1989

Isolation and biological activity of corticostatic peptides (anti-ACTH).

Corticostatins (CS's) are a family of low molecular weight peptides, rich in arginine and cysteine with the ability to inhibit ACTH stimulated adrenocortical steroidogenesis. They show a high degree of specificity in that they do not inhibit the action of angiotensin II in the adrenal cortex. Four corticostatins have been isolated from rabbit lung extracts and peritoneal neutrophil extracts and one from human neutrophils. Among them corticostatin I, CSI, is the most potent with an I.D.50 of 25 nM. Corticostatin activity is different from other published inhibitory factors such as TGF-B and ANF, which inhibit basal and angiotensin II stimulated steroidogenesis. Other factors such as macrophage secreted products, and septic shock plasma factors which have not been fully characterized also have suppressive activity on ACTH induced adrenocortical steroidogenesis in vitro. It is not yet clear whether there is any relationship between corticostatins and these macrophage products and shock plasma factor. Corticostatins do not inhibit dbcAMP induced steroidogenesis, however they do inhibit the accumulation of cAMP in response to ACTH in rat adrenal cell suspensions. Binding studies show that CSI is a competitive inhibitor of ACTH, probably acting by blocking, the address recognition site of the receptor. There is wide variation in the potency of the corticostatins ranging from an ID50 of 100 ng/ml for CSI to a completely inactive analog, HP1. In this paper we will describe the purification of the corticostatins and some recent results obtained on their mechanism of action.

Adrenal Glands↗
PubMed1989

[Component analysis of the extracellular polysaccharide from Mortierella hepiali Chen et Lu sp. nov].

The extracellular polysaccharide from the filtrate of fungal fermentation of Mortierella hepiali, a fungus isolated from traditional Chinese crude drug Cordyceps sinesis, is made up of three sugar components, glucose, mannose and galactose, which are detected by paper and gas chromatography. Both molar ratio and contents of the polysaccharide are determined by the internal standard method.

Animals↗