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Biomedical subjects

Q Yu

Publications and source records attributed to Q Yu.

At least 289 records · Page 16Linked to original sources
PubMed1991

[CA125 and radioimmunoimaging in monitoring of epithelial ovarian carcinoma].

Serum CA125 was measured by monoclonal antibody-OC125 in 50 primary and 29 suspected recurrent ovarian cancer patients. Among the later, RII examination by using 131I labelled polyclonal antibody-OCOV3 was carried out in 16 cases at the same time. For the suspected recurrent cases, the results, as checked by surgico-pathological findings, revealed a corroborative rate of 79.3% for CA125 assay and 87.5% for RII technique. The positive predictive value of both CA125 assay and RII were 100%, while the negative predictive value was only 25% for CA125 assay and 50% for RII technique. However, for each individual case if the results of the two tests were combined, the negative predictive value was also 100%. Preoperative CA125 level had no significant relationship with the prognosis in primary cases. But the two year survival rate for recurrent cases with a preoperative CA125 less than or equal to 35,000 U/L was much higher than those with CA125 greater than 35,000 U/L.

Antibodies, Monoclonal↗
PubMed1990

Basal cell carcinoma syndrome: report of 10 cases.

Ten cases of basal cell carcinoma syndrome are presented. The jaw cysts were proved histopathologically to be odontogenic keratocysts. The multiple naevoid lesions on the skin were identified as basal cell carcinoma in two cases. Skeletal anomalies and intracranial calcification were present in all cases. A positive family history with others affected by the syndrome was traced in two cases.

Adolescent↗
PubMed1990

[Purification and identification of human cardiac myosin light chains].

Normal human left ventricular myocardium was obtained within 5 hours after death. Crude cardiac myosin was extracted from the myocardium by the method of Schossler, and cardiac myosin light chains (CMLC) were purified by isoelectric precipitation, fast protein liquid chromatography and preparative SDS-PAGE, respectively. The three methods were compared. The Ca2(+)-activated ATPase activity of cardiac myosin was 101.4 nmole Pi/mg/min. Molecular weights of CMLCI (24500) and CMLC II (20,000) were identified by analytical SDS-PAGE. All of the results mentioned above were consistent with data reported in the literature. An analytical electrophoresis test of the stability of CMLC stored at -20 degrees C demonstrated that liquid CMLC at long storage times or lower concentrations was easily degraded.

Humans↗
PubMed1990

Are human luteinizing granulosa cells a site of action for progesterone and relaxin?

Specific nuclear staining for progesterone receptor (PR) was detected by immunocytochemistry in human granulosa cells (GCs) obtained from in vitro fertilization protocols. The percent of PR-positive cells (60% to 80%) remained unchanged during 7 days of culture in media containing fetal calf serum, in the absence or presence of human chorionic gonadotropin (hCG) or the progesterone antagonist RU486. Progesterone (P) production by GCs cultured on extracellular matrix from bovine corneal endothelial cells was stimulated by hCG and prostaglandin E2 (PGE2). However, addition of RU486 or human relaxin had no effect on control, hCG-, or PGE2-stimulated P production. Thus, the receptor data are consistent with an autocrine action of P in luteinizing GCs, but initial experiments in cell culture did not define a role for P or relaxin in modulating luteal steroidogenesis.

Cells, Cultured↗
PubMed1990

[The polymorphisms of HTC-defined HLA specificities in the Shanghai Chinese population].

With reference sera and homozygous typing cells (HTCs) of 3rd Asia-Oceania Histocompatibility Workshop Conference, 56 healthy unrelated subjects in Shanghai were typed for HLA-A, B, C, DR, DQ, and Dw. This paper presents the results of HLA-Dw typing, its relationship to serological class II antigens, and the distribution of Dw in the population. The polymorphism patterns of Chinese Dw specificities were quite different from those in Caucasoids and Japanese. The predominant Dw phenotypes detected in Shanghai Chinese were Dw 2, Dw 3, DKT 2, Dw 7 c, (Dw7 + Dw 17) and Dw 23 (DB 5). And significant correlations were observed between Dw 1 and DR 1, Dw 2 and DR 2, Dw 3 and DR 3, Dw 7 c and DR 7, DB 7 and DRw 8, as well as Dw 23 and DR 9. SMY 129, a novel Dw specificity defined by local HTCs and co-studied by the laboratories joined for Dw typing in 3rd AOHWC showed its correlation with DR 5. Nevertheless, more than fifty percent of Dw specificities could not be assigned in the four correspondent designated serological antigens, DR 2, DR 5, DRw 8 and DR 9, respectively, which, together with other blank Dw specificities, gave a total blank Dw gene frequency as high as 43.2% in the population. It was suggested by further analysis that novel Dw specificities might be identified more effectively if efforts would be concentrated on DR 5 and DR 9, two antigen families which, in some way, might represent the characteristics of HLA system in Chinese. Besides, certain HTC-defined antigens, e.g. Dw 3 and the DR 4-related Dw specificities, have been revealed to be in linkage disequilibrium with other DR antigens in addition with the correspondent designated ones, resulting in some unique haplotype combinations in Shanghai Chinese. It seems to us that the particular patterns of polymorphisms of serum- and cell-defined HLA class II antigens would be helpful to elucidate the mechanisms by which certain diseases are in association with HLA in Chinese in a different manner as compared with that in Caucasoids.

Asian People↗
PubMed1990

[Early stage of gan-zheng in children treated with sheng-zhang-Ling].

The authors report here the results of prospective clinic study on 108 cases of the early stage of Gan-Zheng (infantile malnutrition) in children. It was proved that professor Zhan Qisun's tested recipe Sheng-Zhang-Ling(SZL) had an obvious effect in treating children with early stage of Gan-Zheng. The total effective rate was 90.2%, and the significantly effective rate was 60.79%. Various symptoms of patients using this prescription disappeared or improved. The increase of body weight, height and subcutaneous fat of these patients was faster than those of the blank control group and the group using zinc sulfate. The difference was statistically significant among those three groups. It was indicated that effects of the SZL group were not caused by children's natural growth and development. There was an obvious advantage over the zinc sulfate group with regard to clinical effects, side effects and total synthetic effects. It was concluded SZL is an effective prescription for curing patients with early stage of Gan-Zheng and stimulating children's growth and development. Hemoglobin, D-xylose in urine, serum gastrin, serum zinc ion were detected before and after the treatment. It was suggested that SZL had the effects to stimulate gastrointestinal secretion and absorption, to improve digestive function, to increase serum zinc ion and to cure anemia.

Child↗
PubMed1990

[Effect of acupuncture on exercise ability in rabbits].

7 male rabbits were chosen for the experiments. They were tired out on the P20 type table. The blood samples were obtained from the arteria before and after exercise. Blood gas was tested by ABL3 acid-bases balance laboratory made by Denmark's Radiometer Company. The results showed that blood pH and glucose were reduced after exercise, compared with that before exercise (P less than 0.05). The blood was acid. But when they were given acupuncture on Shenshu (U.B. 23) point bilateral, their exercise-duration under the same intensity was prolonged (P less than 0.05), blood glucose restored, and blood acid-base balance was relatively stable. The complexity of this mechanism needs further study.

Acupuncture Therapy↗
PubMed1989

Diagnostic value of computed tomography in parotid tumours.

In 18 cases of parotid tumours, CT demonstrated their primary site, extent and invasion of surrounding tissue precisely. It was valuable in the majority of cases in differentiating benign from malignant tumours and could distinguish accurately intrinsic from extrinsic. CT sialography was superior in equivocal cases when it made the tumour easier to identify and the diagnosis more accurate.

Diagnosis, Differential↗
PubMed1989

Studies on the mechanism of anemia in rodent malaria.

The underlying cause of anemia is one of the problems to be solved in malaria research. Many factors are involved in reducing the quantity of uninfected red blood cells (RBC) in addition to those infected RBC destroyed by malaria parasites. In the Plasmodium yoelii (P.y.)-mouse model, the amount of [51Cr]-labelled normal mouse RBC destroyed in peripheral blood as well as the quantity phagocytized by spleen cells during acute and chronic infection in vivo is reported in this paper. Our results show that compensatory enlargement of the spleen, which cleans up a large amount of the damaged uninfected RBC, may be the major cause of anemia in chronic malaria infection. In acute malaria infection destruction of uninfected RBC in peripheral circulation is higher than that in normal mice. Neither malaria antigen, mouse autoantibody nor immune complex was detected on the surface of normal RBC from infected mice using indirect immunofluorescence assay (IFA) or [3H]-isoleucine-labelled P.y. antigen (P.y.Ag) in vitro. This suggests that malaria immune complexes do not play an important part in RBC destruction in circulating blood. Since no obvious hemolysis was observed by mixing RBC with P.y. culture supernatant in vitro, it is possible that physical and chemical changes in uninfected RBC induced by malaria metabolites are the prerequisite for their destruction in circulating blood in vivo. Hemolysis occurs due to external stresses, such as those incurred when damaged RBC run into each other in the blood stream or when they change their shape to pass through capillaries.

Anemia↗
PubMed1987

Molecular mapping of point mutations in the period gene that stop or speed up biological clocks in Drosophila melanogaster.

The pero1 and the pers mutations in Drosophila melanogaster, which seem to eliminate or speed up, respectively, the clocks underlying biological rhythmicity, were mapped to single nucleotides. Chimeric DNA fragments consisting of well-defined wild-type plus mutant DNA subsegments were constructed, introduced into flies by germ-line transformation, and assayed for biological activity. These experiments localized both pero1 and pers to a 1.7-kilobase DNA fragment that is mostly coding DNA. Sequencing of this subsegment from each mutant showed that pero1 is completely accounted for by a nonsense mutation in the third coding exon of a 4.5-kilobase RNA transcribed from this locus. The pers mutation is also a single nucleotide substitution, in the fourth coding exon, which results in a serine-to-asparagine substitution in the per gene protein product. The functional significance of these changes is discussed with reference to the phenotypes of the two mutations.

Amino Acid Sequence↗
PubMed1986

Germ-line transformation involving DNA from the period locus in Drosophila melanogaster: overlapping genomic fragments that restore circadian and ultradian rhythmicity to per0 and per- mutants.

P-element-mediated transformations involving DNA fragments from the period (per) clock gene of Drosophila melanogaster have shown that several subsegments of the locus restore rhythmicity to per0 or per- mutants. Such fragments overlap in a genomic region complementary to one transcript, a 4.5-kb RNA which is probably the per message, in that it is necessary and (in terms of expression from this X-chromosomal locus) sufficient for the fly's circadian rhythms. It is also at least necessary for the high-frequency oscillations normally produced by courting males as they vibrate their wings. The entirety of the 4.5-kb transcript is not necessary for rather strong rhythmicity; nor does it seem to be sufficient, in transformants, for wild-type behavioral phenotypes. A 0.9-kb RNA, homologous to genomic region immediately adjacent to the source of the 4.5-kb species, oscillates in its abundance over the course of a day; but coverage of this transcript source in several transformants carrying a per0 mutation--which eliminates the 0.9-kb RNA's oscillation--does not restore rhythmicity. All of the independently isolated arrhythmic mutations tested were covered by the same array of overlapping per+-derived DNA fragments, implying that the only portion of the locus which has mutated to arrhythmicity is complementary to the 4.5-kb transcript.

Animals↗