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Biomedical subjects

Q Wu

Publications and source records attributed to Q Wu.

At least 433 records · Page 24Linked to original sources
PubMed1990

[Purification and identification of human leukocyte myeloperoxidase].

Myeloperoxidase (MPO) plays an important role in the oxygen-dependent microbicidal mechanism of polymorphonuclear neutrophils. The purpose of our study was to investigate the therapeutic potency of human MPO preparations. This paper is to present our work on MPO purification and its identification. White blood cells, isolated freshly from normal donors, were lysed with cetyltrimethylammonium bromide to liberate myeloperoxidase. The purified MPO was obtained by 65% (NH4)2SO4 precipitation followed by separation over the Sephadex G150 column. These pure MPO preparations were green in color and had an A430/A280 nm of 0.68. The enzymatic activity was of 29.77 u/mg. The pure normal MPO was composed of a 59,000 MW peptide, a 13,500 MW peptide, and a 38,000 MW peptide when subjected to SDS-PAGE under reducing conditions. The study of the therapeutic effect of the pure MPO on mice with C. albicans infection is under way.

Electrophoresis, Polyacrylamide Gel↗
PubMed1990

[The effects of needling acupoint, nonpain and pain stimulation on electroencephalogram (EEG) of somatosensory area analysed by computer].

The present study was to observe the change of EEG power spectrum during acupuncturing, nonpain stimulating and pain stimulating; then to comparatively analyse the effects of acupuncturing acupoint on brain function. The work was performed on 30 normal subjects. Nonpain or pain stimulation was applied to a medial finger, and the intensity of stimulation was determined by subject's report. Acupoints were "Neiguan" and "Jianshi". EEGs were recorded on a tape recorder before, during and after each stimulation. Then EEG power spectrum were analysed by SM-2100 computer. The results were as follows: According to the analysis of EEG power spectrum, in most of subjects, alpha (a) band was shifted to higher band during pain stimulating, there was significant change, P less than 0.05. The highness of a spike was significantly increased during acupuncturing acupoints, P less than 0.02. The ratio of delta power spectra to theta (theta) and delta (delta) power spectra showed increased tendency during acupuncturing or nonpain stimulating, but decreased during pain stimulating. It was indicated that nociceptive pain-stimulation resulted in hyposynchrony of EEG: frequency was increased, amplitude was decreased, alpha rhythm was inhibited to a certain extent; acupuncturing acupoints had the effect which could strengthen the stability of EEG rhythmic activities. We know that EEG rhythmic activities show clearly the stability of balance inside organism. So this result suggested that acupuncturing acupoint could play a role in modulating the balance inside organism and enhancing the stability of brain function.

Acupuncture Points↗
PubMed1990

[Purification and identification of antimicrobial peptides of rabbit neutrophils].

Five antimicrobial peptides were extracted from the cytoplasmic granules of rabbit neutrophils with 0.01 mol/L citric acid. SDS-PAGE gel densitometric analysis of the citric acid extract showed that they accounted for 34%-42% of the total extract proteins. When subjected to acid urea polyacrylamide gel electrophoresis, five peptides migrated much further to the cathode than lysozyme. Beginning with the most cathodal component, they were in turn referred to as NP1, NP2, NP3, NP4, and NP5. They were purified from the citric acid extract by virtue of preparative polyacrylamide gel electrophoresis. Each of them was of low molecular weight (2,500-6,000) as determined by SDS-PAGE. Three of the peptides were tested in vitro for fungicidal activity. When Candida albicans (10(7)/ml) were incubated with 12.5 micrograms/ml of NP1 or NP2 at 37 degrees C for 2 h, the death rates of the organisms were 96.6% and 93.3% respectively. NP3 was shown to be active against Candida albicans although less potent than NP1 and NP2.

Animals↗
PubMed1990

[Effect of blood-activating and stasis-removing drugs on hypercoagulability data in nephrotic syndrome].

UNLABELLED: Platelet aggregation test (PAgT), plasma factor VIII related antigen (VIII R:Ag) and kaolin partial thromboplastic time (KPTT) in 32 nephrotic children were determined. RESULTS: (1) PAgT induced by ADP and adrenaline which included 1 minute and 5 minute aggregation rate in the patients (30. 78 +/- 7.44%, 72.56 +/- 18.09%, 22.16 +/- 9.24%, 67.53 +/- 22.32%) was significantly increased (P less than 0.02-0.001) as compared with the normal control group (30.65 +/- 8.38%, 57.98 +/- 13.60%, 18.14 +/- 7.33%, 55.92 +/- 16.10%) except 1 minute aggregation rate induced by ADP (P greater than 0.05). (2) VIII R:Ag and KPTT in patients (226.97 +/- 50.47%, 32.04 +/- 5.88 sec) were obviously different (P less than 0.001, P less than 0.02) from the normal control group (107.11 +/- 24.55%, 35.42 +/- 5.00 sec). The results suggested that PAgT, VIII R:Ag and KPTT could be used as laboratory data reflecting the hypercoagulable state in nephrotic children. According to their age, sex and the clinical types of nephrotic syndrome, 24 nephrotic children with abnormality of PAgT, VIII R:Ag and KPTT were random divided into group 1 given prednisone only and group 2 given prednisone, blood-activating and stasis-removing drugs. RESULTS: the difference of PAgT, VIII R:Ag and KPTT in two groups between pre- and post-treatment was significant statistically (P less than 0.05-P less than 0.001). The decline in PAgT and VIII R:Ag was faster in group 2 than in group 1 (P less than 0.05-P less than 0.01). The results showed that blood-activating and stasis-removing drugs were likely to improve abnormal PAgT and VIII R:Ag in nephrotic children.

Child↗
PubMed1990

[Analysis of musk rheumatic oil by GC/FTIR].

This paper studied musk rheumatic oil by GC/FTIR. Ten main components were identified qualitatively, and methyl salicylate, eugenol, cinnamaldehyde were determined by GC internal standard method quantitatively. Average recoveries are within 100 +/- 1.0%, variation coefficients are less than 2.0%.

Acrolein↗
PubMed1989

The early B lineage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule.

Biochemical similarities and cellular distribution patterns of the early B lineage-specific BP-1 alloantigen and the B lineage transformation-associated 6C3 Ag prompted this comparative study of the reactivities of the BP-1 and 6C3 mAb. Both Ag were found to be expressed on the same cells in normal tissues, and on the same cell lines when a large panel was analyzed. The Ag are both phosphorylated and have identical m.w., which may vary in different cell types because of differences in glycosylation. Immunoprecipitation of pre-B cell lysates with the BP-1 antibody removed the 6C3-reactive molecules and vice versa. However, the 6C3 antibody did not inhibit binding of the BP-1 antibody to viable cells and, in fact, enhanced immunofluorescence staining was observed when both antibodies were added together. These results indicate that the BP-1 and 6C3 antibodies react with different epitopes on the same molecule that is expressed in relatively low levels on normal early B lineage cells, and in relatively high levels on most neoplastic pre-B cells, pre-B cells in long term bone marrow cultures and certain stromal cell lines.

Animals↗
PubMed1989

[Correlation of steroid hormone receptors (SR) with clinical and pathological features of human breast cancer].

Paraffin-embedded materials from 104 patients with breast cancer were assayed for the expression of estrogen receptor (ER), progesterone receptor (PR) and androgen receptor (AR) with avidin-biotin complex method. A significant positive correlation was found between SR status and cell differentiation or lymph node status. Tumors with elastosis tend to contain ER, PR and AR more frequently. Patients with positive ER, PR or AR tumors were 1.2 to 2.6 times more likely to survive than those with negative ones. The five-year survival rate increased with the increase of number and amount of positive SR. These results indicate that ER, PR and AR detected by ABC method may be used as biomarkers for tumor differentiation and have prognostic values in breast cancer.

Adult↗
PubMed1987

Formation of lymphocyte colonies under serum-free culture conditions in normal individuals and patients with chronic lymphocytic leukemia.

This paper describes a culture system which supports the formation of B cell and some T cell colonies under serum-free conditions in peripheral blood samples of normal individuals and patients with chronic lymphocytic leukemia (CLL) of B cell type. In this system, serum is replaced by bovine serum albumin, transferrin, cholesterol, insulin and catalase or horseradish peroxidase. In addition, it is necessary to add staphylococcus protein A, mitomycin-treated T cells as feeders and phytohemagglutinin leukocyte-conditioned medium as a source of growth factors. The plating efficiency is greatly enhanced when normal cells are incubated with galactose oxidase prior to plating and when CLL cells are exposed sequentially to neuraminidase and galactose oxidase.

B-Lymphocytes↗
PubMed1986

Mobile phase effects on the separations of substituted anilines with a beta-cyclodextrin-bonded column.

The capacity factors of several substituted anilines were measured on a beta-cyclodextrin (beta-CD)-bonded column with mobile phases varying from the classical, normal-phase condition, e.g. n-heptane-2-propanol, to the classical, reversed-phase condition, e.g. water-2-propanol. Different modifiers, such as acetonitrile, methanol, and tetrahydrofuran and a Partisil PXS-ODS column were also used for comparison. In general, it was found that the beta-CD column, having the ability to form inclusion complexes with certain substrates is better and more selective in the reversed-phase separation of many aromatic compounds than the Partisil PXS-ODS column. The normal-phase separation on beta-CD was even more efficient, owing to the presence of large number of hydroxy groups on the surface of beta-CD molecules and the more rapid mass transfer in the column. In the present case, alcohols were found to be better modifiers than aprotic solvents. The effects of organic solvents on both normal-and reversed-phase separations are also discussed. The minima observed for the log k' vs. percent organic solvent plots for a number of substituted anilines are reasoned to originate from solute-solvent competition, which interacts with the stationary phase, as well as from the "relative solubility" of solutes in the stationary and mobile phases.

Aniline Compounds↗
PubMed1985

Selenium intake and metabolic balance of 10 men from a low selenium area of China.

Selenium intake and urinary and fecal Se excretion of 10 healthy men from a low Se area in China were determined for three consecutive days, in summer, fall, and winter of 1983, and the spring of 1984 while self-selected diets were being consumed. Mean daily Se intake was 8.8 micrograms/day with a range of 2.3-35.5 micrograms/day, and was far below the recommended range of safe and adequate Se intake of 50-200 micrograms Se/day (National Academy of Sciences/National Research Council). Mean urinary and fecal Se outputs were 3.7 and 3.4 micrograms Se/day, respectively. Mean Se balance during this time was +1.8 micrograms Se/day. Apparent absorption of Se approximated 57%. The low Se intake in this area is a cause for concern since the residents of Molimo may be at risk for Se deficiency diseases.

Adult↗
PubMed1985

Bioavailability of selenium to residents in a low-selenium area of China.

For 8 wk 5 groups of 10 men each were given 0.5 g/day DL-methionine, 150 micrograms Se/day as sodium selenite with or without methionine or 150 micrograms Se/day as selenomethionine with or without methionine. Twenty subjects received placebo as controls. Initially plasma Se rose more rapidly than RBC Se. Increases in Se levels were significantly greater with selenomethionine than with the selenite supplement. In the placebo and methionine supplemented groups neither plasma nor RBC Se varied significantly over the course of the study. Supplementation with selenium resulted in marked increases in plasma and RBC GSH-Px within 2 and 4 wk, respectively. Plasma and RBC GSH-Px activity did not differ significantly between Se-supplemented groups. These studies suggest that selenomethionine-Se was more effective in raising plasma and RBC Se than was selenite-Se. Methionine supplements may increase the bioavailability of selenium in severely deficient subjects.

Adult↗
PubMed1982

Studies on optic nerve of Lipotes vexillifer-structure, fiber count and diameter spectrum.

Four optic nerves obtained from two Lipotes Vexillifer have been studied with the aid of electron microscope. The fiber diameter spectra of all the four optic nerves are unimodal. The diameters of fibers range from 0.35 to 7.45 microns with the highest frequency at 1.5 microns. T test indicates there is no significant difference in fiber density and fiber diameter between the central and peripheral regions of the optic nerve. The ratio of axon diameter to the total fiber diameter is 0.6 +/- 0.02 (p less than 0.05). The examination of four optic nerves indicates a mean count of 78,900 fibers; none of unmyelinated fibers have been seen. The shapes of the cross sections of fibers are very irregular. The myelin sheaths of some nerve fibers are very thick while those of some others are thinner.

Animals↗