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Biomedical subjects

Q S Yuan

Publications and source records attributed to Q S Yuan.

5 recordsLinked to original sources
PubMed2000

[Fusion and expression of the gene encoding human Mn-SOD to anti-CEA single-chain antibody in Escherichia coli].

The gene encoding human manganese-superoxide dismutase (Mn-SOD) was fused to anti-carcinoembryonic antigen single-chain antibody gene to construct the fusion gene, then was ligated into prokaryotic expression vector pET-22b(+), The fusion gene was expressed in E. coli at high level, accounting for 24% of the total bacteria soluble protein; and was characterized by SDS-PAGE and Western-blot analysis; the expression product had the CEA-binding ability in RIA, and also had the SOD activity by pyrogallol autoxidation assay. So, the Mn-SOD moiety retains substantial enzymatic activity, where the ScFv moiety can deliver the fusion protein to tumor, Mn-SOD is a potential tumor-suppressor gene, maybe the fusion protein can provide a new pathway to tumor therapy.

Amino Acid Sequence↗
PubMed1998

Refining the scale-up of chromatographic separations.

The use of heavily loaded columns and complex processing conditions makes scale-up of chromatographic separations a non-trivial process. The wide ranges of process conditions that must be investigated demands that a large number of preliminary experiments must usually be made in small columns and laboratory-scale work stations. These preliminary data can be biased by improper column packing, poor distributors and dispersion in auxiliary apparatus, and it is important to understand these disturbing factors in detail. Moreover, it is precisely at this macroscopic level that our understanding of the chromatographic process is weakest, for large columns as well as small. This paper addresses three of these factors: Efficient elimination of peripheral effects and characterization of both header flow distribution and packing non-uniformity. This will be done using a variety of experimental and analytical approaches including nuclear magnetic resonance imaging, computational fluid dynamics and mass transfer, and careful experimentation.

Acetone↗
PubMed1990

Tooth surface fitting and three-dimensional display using hidden surface removal technique.

Bi-cubic parametric spline surface is applied for surface-fitting of the external surface and canal of tooth based on its crown-to-root, cross-sectional data obtained by X-ray scanning. For three-dimensional display of the fitted surfaces, a technique for fast removal of the hidden surfaces is also reported. The selection of the accumulated chord length or coordinate component as parameter make it possible to fit any kind of surface smoothly. For displaying or plotting a well-fitted surface, a method capable of discriminating the grades for sequential removal of the hidden surfaces is developed for substantial reduction of computing time.

Algorithms↗