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Biomedical subjects

Q Liu

Publications and source records attributed to Q Liu.

At least 721 records · Page 40Linked to original sources
PubMed1991

[Anti-inflammatory, analgesic and sedative effects of Leontice kiangnanensis P.L. Chiu].

The ethanolic extract of Leontice kiangnanensis (EELK) can inhibit obviously the increased permeability of abdominal blood capillaries and the ear inflammation in mice as well as the swelling of hind paw and the proliferation of granuloma induced by cotton-pellets in rats, EELK can also raise the pain thresholds during hot-plate, formaldehyde and writhing tests in mice. Use of EELK together with pentobarbital may enhance sedative effect on mice.

Animals↗
PubMed1990

Prenatal identification of potential donors for umbilical cord blood transplantation for Fanconi anemia.

Reported here are studies of Fanconi anemia fetal cells that led to the first use of umbilical cord blood for hematopoietic reconstitution in a clinical trial. Prenatal diagnosis and HLA typing were performed in fetuses at risk for Fanconi anemia (FA) to identify, prior to birth, those that were unaffected with the syndrome and were HLA-identical to affected siblings. Umbilical cord blood was harvested at the delivery of these infants; assays of progenitor cells indicated the presence of colony-forming units-granulocyte-macrophage (CFU-GM) in numbers similar to those of bone marrow CFU-GM that are associated with successful engraftment in HLA-matched allogeneic bone marrow transplantation. The possibility that umbilical cord blood from a single individual can be used as an alternative to bone marrow for hematopoietic reconstitution has now been demonstrated by the successful engraftment of two patients with FA. Progenitor cell assays of umbilical cord blood collected at the birth of a child affected with FA, who had been misdiagnosed on the basis of chorionic villus sampling (CVS) studies, indicated a profound deficiency in colony formation, consistent with previously reported abnormalities in the growth of FA cells in vitro. These results suggest that the hematopoietic disorder in FA is related to an underlying problem with cell proliferation.

Blood Cell Count↗
PubMed1990

Quantitative structure-activity relationships for calmodulin inhibitors.

Using the discriminant analysis method, we completely distinguished 24 calmodulin inhibitors in three groups, as classified by Zimmer et al. The resultant discriminant functions distinguished the three groups in terms of positive potential surface area on the side chain, as well as the total and neutral surface areas on the ring in the inhibitor molecules. Group assignment of additional calmodulin inhibitors from other sources was then estimated according to the discriminant functions. The relationship between structure and inhibitory potency on calmodulin-activated phosphodiesterase for group I inhibitors, together with those estimated, was studied using the adaptive least squares method with several parameters dependent on molecular conformations. A "best conformer" was selected for each inhibitor on the basis of quantitative structure-activity relationship (QSAR). The results of QSAR analysis of group I inhibitors showed that hydrophobicity was important for the ring moiety but not for the side chain. The negative potential surface area of the side chain is necessary for activity. It is desirable for the nitrogen atom in the side chain, which is considered the center of the negative potential area, to be located far from the ring moiety. Thus, the ring moiety and side chain may possibly play different roles in interactions with the receptor system.

Calmodulin↗
PubMed1990

Effects of clothing values on perceived clothing deprivation among adolescents.

The present purpose was to investigate the relationship between clothing values and perceived clothing deprivation among adolescents. From previous studies a questionnaire was developed to measure perceived clothing deprivation (the dependent variable) and clothing values (the independent variable). Items were measured on a 5-point Likert scale. The sample was comprised of 338 high school students enrolled in Grades 9 to 12 home economics courses at six high schools. The effects of values (aesthetic, economic, exploratory, political, religious, sensory, social, and theoretical) on perceived clothing deprivation were tested. Pearson correlations and multiple regression indicated positive relationships between perceived clothing deprivation and the economic and social clothing values. Also, these values accounted for significant proportions of the variance of perceived clothing deprivation. Negative relationships between aesthetic and political clothing values and perceived clothing deprivation were noted; however, they did not contribute significantly to the variance of perceived clothing deprivation. No relationships were found between the remaining clothing values and perceived clothing deprivation.

Adolescent↗
PubMed1990

[Application of 13CNMR to structural identification of the flavonoid glycosides].

13CNMR has become an important method for the structural determination of organic natural products. It is especially useful for identifying the structure of glycosides. This paper deals with the application of 13CNMR to the identification of the flavonoid glycosides isolated from the roots of Glycyrrhiza eurycarpa P.C. Li. The discrimination of chemical shifts of C-2, C-3 and C-4 (carbonyl) signals for different types of flavonoids, such as isoflavones, flavanones, chalcones, flavones and pterocarpans is discussed. The application of 13CNMR in the determination of structure and configuration of sugar moieties, for determination of glycosidic linkage, and for confirmation of the position of glycosidation and linkage between two sugar moieties are also discussed briefly.

Carbon Radioisotopes↗
PubMed1990

Cloning and identification of a rabbit sperm membrane protein gene.

Antibodies raised against rabbit sperm membrane protein (rSMP-B) interacted with a rat testis cytosolic protein with an estimated molecular weight of 70 kD. The clone expressing rSMP-B was isolated by epitope selection from a rat testis lambda gt11 vector cDNA library. An Eco R1-cut 2.0 kb cDNA fragment encoding rSMP-B was identified. The cDNA fragment (RSD-1) was isolated, subcloned and restriction mapped. Dot hybridization analysis of mRNA prepared from human, rabbit and rat testes showed that the gene encoding rSMP-B is expressed in the testes of all three mammals.

Animals↗
PubMed1990

[Study on the variation of relaxation time in diabetic cataractous lenses of rats by using NMRA].

Nuclear magnetic resonance analysis (NMRA) was used to measure the relaxation time T1 and T2 of the protons in the different-stage-diseased lenses of rats with diabetic cataract caused by the streptozotocin, and the results were put in comparison with those obtained from the control group, showing that after one month of medication the values of T1 and T2 in the cataractous lenses are greater than those gained from the control group. The variation, as time goes on, becomes greater and greater. T1 and T2 indicate the speed of the free induction decay (FID) signals, and the variation of the FID signals are in direct proportion to that of the proton content of the samples. This shows that an increase in T1 and T2 indicates an increase in the water content in the diseased lenses, and the changes in ratio of "bound" water to free water. Such changes may be explained that the space for "bound" water is occupied by some other macromolecules, causing the alteration of the macromolecular structure, and consequently the aggregation of protein and the degeneration or dehydration or dehydration of the membrane occur, which may give rise to another scattering centre. So the scattering of the lens increases and its transparency decreases. This result is in agreement with some reports in the medical literature. As T1 and T2 are quite different in the normal and pathological tissues, and the protons are the most extensive nucleus in all biological tissues, NMR is a very important method to determine the proton relaxation time in the biological tissue and to judge if it is normal or not.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

Rapid serodiagnosis for leprosy--a preliminary study on latex agglutination test.

In this study, we have developed two latex agglutination tests (LATs) with phenolic glycolipid-I (PGL-I) and natural disaccharide-octyl-bovine serum albumin (ND-O-BSA) as antigens in 110 leprosy patients (LL = 30, BL = 30, BT = 30, and TT = 20), 50 tuberculosis cases, and 30 normal controls. These two LATs were compared with corresponding ELISAs (ND-O-BSA ELISA and PGL-I ELISA) and analyzed by the chi-squared test. There were no significant differences between the two LATs (PGL-I LAT and ND-O-BSA LAT) and their corresponding ELISAs. There was an increase in the proportion of positive cases detectable which coincided with the clinical classification of leprosy, i.e., lepromatous cases were more likely to be positive than tuberculoid cases. LATs are more simple and rapid than ELISAs and have high sensitivity (77% in ND-O-BSA LAT, 80.5% in PGL-I LAT) and specificity (99% in both LATs). LATs may become useful tools for the immunodiagnosis of leprosy in the field. The stability and repeatability of LATs are discussed in detail.

Antibodies, Bacterial↗
PubMed1990

[New approach for management of retinal detachment with macular hole].

Three kinds of unconventional methods were used to treat retinal detachment with macular hole in 17 cases (17 eyes). A combination of vitrectomy, transscleral drainage and intraocular air injection was used for the cases of retinal detachment with macular hole and evident vitreous traction. Transscleral drainage combined with air injection was used for those of macular hole without vitreous traction. For the cases of combined peripheral and macular holes, we treated only peripheral holes, ignoring the macular hole. After a 3-16 months follow-up, 15 out of the 17 eyes obtained retinal anatomic reattachment (88.2%), 10 eyes had their vision improved, 4 eyes remained unchanged, 9 eyes obtained a visual acuity of better than 0.05 postoperatively. Compared with the conventional method of macular buckling, these procedures have the advantages of less damage to macula, less complications and more satisfactory visual improvement.

Adult↗
PubMed1990

[A study on treatment of retinal detachment with unseen retinal breaks].

64 cases (66 eyes) of retinal detachment in which no breaks can be found were treated with pars plana vitrectomy, scleral buckling or encircling procedures. The selection of operation depended on the shape and size of retinal detachment, the position of peripheral retinal lattice degeneration, phakia or aphakia, traumatic situation and the appearance of proliferative vitreoretinopathy. The results showed that the detached retina was successfully reattached in 53 eyes (80.3%), 4 eyes were improved (6%), only 9 eyes failed (16.7%). The technic of operation and application are discussed. We consider that suitable active surgical treatment for this kind of cases is encouraging.

Adolescent↗
PubMed1990

[Pharmacologic studies on qiongjiang wine].

In this article, preliminary pharmacologic studies of Qiongjiang wine are reported a comparison of this wine with Guilingji wine is made. The results show that Qiongjiang wine has the action of roborant and sex hormones. It can improve the phagocytic function of macrophage in the abdominal cavity of mice. No toxic response has been found in acute toxicity test.

Alcoholic Beverages↗
PubMed1990

[Detection of sorbitol content in crystalline lens of normal rats and rats with diabetic cataract by 1H-NMR].

The pulse Fourier NMR was employed to measure the artificial diabetic cataract lens at various stages of its formation, and the lenses of the normal rats. Data obtained by using this method show that all the peaks that of water concentrate in the range of delta less than 4 ppm. The peak value at delta = 3.20 ppm is on a marked increase during the formation of cataract which is caused by the phosphate metabolites, such as GPC, ATP, ... etc, in cataract lens. With the development of the disease, the peak width at delta = 3.73 ppm becomes greater and greater, which shows that the activity of sorbitol dehydrogenase has decreased. This leads to a high concentration of the sorbitol in the cataract lens. Consequently, the osmosis pressure in the cataract lens is increased, and excessive water might dip into the crystalline lens to keep the balance of the osmosis pressure. And this might result in the hydration of the fiber cell of the crystalline lens, which might cause a swelling or blisters. These results are in favour of the prolongation of the relaxation time of cataractous lens reported in our other papers, and also support those gained by biochemical studies issued in the medical literature.

Animals↗
PubMed1989

[Studies on chemical constituents of Glycyrrhiza eurycarpa P. C. Li].

A species of the genus Glycyrrhiza, G. eurycarpa P. C. Li recently reported as a new species growing in Gansu Province and Xinjiang Autonomous Region has not been studied before on its chemical constituents. This paper reports the isolation and chemical elucidation of four flavonoid glycosides from this species collected in Jinta County, Gansu Province. On the basis of physical and chemical constants and spectroscopic data (UV, IR, MS, 1HNMR and 13CNMR), compound I was confirmed as a new isoflavone diglycoside. Its structure was elucidated as formononetin-7-O[-D-apio-beta-D-furanosyl(1----2)]-beta-D-gl ucopyranoside. The new compound was named glycyroside. The other three components (II-IV) were identified as liquiritin, isoliquiritin and schaftoside, respectively.

Chemical Phenomena↗
PubMed1989

Evaluation of fluorescent leprosy antibody absorption test versus enzyme-linked immunosorbent assay with phenolic glycolipid 1 and their use in immuno-epidemiological studies on leprosy.

We systematically conducted comparative studies on the validity, reliability and practicality of FLA-ABS.T/PGI-ELISA in large samples. Namely, 284 leprosy patients, 20 tuberculosis patients, 172 normal controls (from nonendemic area of leprosy), 425 leprosy household contacts (HC) and 2573 random samples from the general population (RS) were involved. The results indicated that FLA-ABS.T/PGI-ELISA are highly sensitive and specific for detecting antibodies against M. leprae. Their Youden's indexes (YI) are greater than 90%, and the positive predicative and negative values are 90%. The test results agreed with immuno-epidemiological studies: 1. The positive rates using FLA-ABS.T/PGI-ELISA increased gradually from TT to LL leprosy patients (in HC, the positive rates of PGI-ELISA were much higher in contacts of multibacillary patients than in contacts of paucibacillary patients); 2. The positive rates detected by FLA-ABS.T were identical to those of PGI-ELISA both in HC and in RS; 3. Among RS, the positive rates detected by FLA-ABS. T/PGI-ELISA were similar in each district and were in concordance with the general prevalence rates. Thus, both FLA-ABS.T and PGI-ELISA are useful tests in diagnosing leprosy and detecting subclinical infection with M. leprae. However, because the PGI-ELISA is simple, it will be more practical than FLA-ABS.T in the future. The authors emphasize that the methodology of obtaining dried blood from ear lobes is important for the immuno-epidemiological study of leprosy on a large scale.

Antigens, Bacterial↗
PubMed1989

[Microvitreous surgery combined with scleral encircling for the treatment of complex retinal detachments].

50 cases (50 eyes) of various complex retinal detachments (RD) were treated with a combination of vitrectomy, membrane peeling, intraocular air tamponade and scleral encircling. RD with opaque media, RD with proliferative vitroretinopathy, traction RD due to trauma or other factors, RD with posterior breaks, and giant tears were included. During the follow-up periods of 3-12 months, 27 cases (54%) obtained anatomic reattachment, 23 failed (46%). Of the successful case, 24 eyes achieved improvement, 2 eyes remained unchanged, and one eye became worse in their vision. The surgery complications were: retinal haemorrhage, choroidal haemorrhage, iatrogenic tears, and the development of cataract.

Adolescent↗
PubMed1989

[Studies on harmful microbes in recirculating cooling water system of oil refinery].

The microbiol counts, type, distribution as well as interrelation between microbial counts and biofouling of the main harmful microbes including slimeforming heterotrophic bacteria, sulfate reducing bacteria, iron bacteria and fungi has been studies in 16 oil refineries of China. The control guideline of the harmful microbes for recirculating cooling water system of refinery were suggested, that is slime-forming bacteria less than 10(5)/ml, sulfate reducing bacteria less than 10(2)/ml. iron bacteria less than 10(3)/ml, fungi less than 10/ml. The appearing rate of the predominant strains from cooling water system of various refineries were calculated and identified and the composition of the main harmful representative microbes in cooling water system were determined.

Cold Temperature↗