Search PubMed⌕ Search

Biomedical subjects

Q Liao

Publications and source records attributed to Q Liao.

62 records · Page 4Linked to original sources
PubMed1992

[Preparation of human placenta microvillous membrane and the study of its transferrin receptor].

Human placenta microvillous membrane (PMM) was prepared by differential centrifugation and by sucrose gradient centrifugation, and the transferrin receptor (TfR) was studied using the receptor radioassay with 125I-transferrin as a radioligand. The factors affecting ligand-receptor binding reaction and the characteristics of TfR were also studied. The result showed that there was no significant differences (P > 0.1) in specific binding rates of 125I-transferrin to its receptor between the two membrane preparations, indicating that both of them could be used for TfR analysis, but the method of differential centrifugation was more simple and less time consuming. The study of TfR in sixty cases showed that the TfR binding sites on PMM and Bmax were 3.53 +/- 1.98 x 10(12) sites/mg membrane protein and 6.33 +/- 4.21 x 10(-12) mol/mg membrane protein, respectively. The Kd was 4.95 +/- 3.39 x 10(-9) mol/L, and the highest specific binding was 26% with nonspecific binding less than 3%. The conditions for ligand-receptor binding reaction were optimized when the concentrations of membrane protein and the 125I-transferrin in each test tube were 50 micrograms and 50,000 cpm (specific radioactivity being 2109 kBq/micrograms transferrin) respectively the incubation time was 30 min, and the concentration of polyethylene glycol added for separating B/F was 12% (W/V). transferrin binding to its receptor was characterized by high affinity, high specificity and being saturated.

Cell Membrane↗
PubMed1991

[Investigation of impairment of neutrophil's phagocytosis and bactericidal function in rats with iron deficiency].

Studies were made to determine the neutrophil's phagocytosis and bactericidal function in three groups of rats (control, iron deficiency, and iron supplement). Results showed that there were significant differences in values of chemiluminescence (CL) among three groups. The values of peak CL and five minutes integrated CL were markedly decreased in neutrophils of iron-deficient rats, accounting for only 41% and 32% of the control's values respectively. These suggested that the activity of NADPH oxidase was decreased, and the function of respiratory burst of neutrophils was impaired. The activity of myeloperoxidase in the iron-deficient neutrophils was also significantly lower than that in the control cells. It constituted only 30% of the control's value, indicating that the bactericidal function of neutrophils was injured. One week after iron administration, the low values of the peak CL, the five minutes integrated CL and the activity of myeloperoxidase all went up apparently, but not reached the normal levels yet. The time the function of neutrophils in iron-deficient rats returned to normal may be related to the process of neutrophil maturation in bone marrow.

Anemia, Hypochromic↗
PubMed1990

[Influence of iron deficiency anemia on development of thymus and spleen and adenosine deaminase activity in rats].

Models of rats with iron deficiency anemia (IDA) were established, and changes in development of the thymus and spleen and adenosine deaminase (ADA) activity in them studied. The size and weight of the thymus in IDA rats were decreased, which suggested that the development of the thymus was lowered; but the size and weight of the spleen in IDA group were increased. ADA activities of the thymus and the spleen in IDA rats were significantly decreased by 32.98% and 25.89%, respectively. One week after iron supplement, the weight of the thymus and spleen in IDS group returned to normal level (P greater than 0.05), and their ADA activity also significantly increased, but ADA activity of the thymus tissue did not return to the normal. The results suggested that the changes in ADA activity of the thymus and spleen tissues were related to iron deficiency. Stepwise regression analysis suggested that there was a negative correlation between ADA activity of the thymus tissue and FEP/Hb ratio.

Adenosine Deaminase↗
PubMedDate not supplied

Function of estrogen receptor isoforms alpha and beta in endometrial carcinoma cells.

The aim of this study was to explore how to modulate the expression of estrogen receptors (ER) alpha and beta and to verify the role of ERalpha and beta in relationship to estrogen and tamoxifen (TAM). A series of oligodeoxyribonucleotides (ODN) corresponding to regions of the ERalpha or beta was tested in human endometrial cancer cell lines (HEC-1B). The change in HEC-1B proliferation in response to 17beta-estradiol (E(2)) and TAM under the impact of antisense ODN was studied. The results of the study are as follows: 1) transfection with antisense ODN significantly inhibited ERalpha and ERbeta protein production, 2) the cells lost the ability to proliferate in response to E(2) after transfection with ERalpha antisense ODN especially at 24, 48, and 72 h. There was no obvious change in response to E(2) in HEC-1B cell lines that were transfected with ERbeta antisense ODN, and 3) after transfection with ERalpha antisense ODN, HEC-1B cells lost the ability to proliferate in response to TAM at 48 h. This inhibition was also observed after transfection with ERbeta antisense ODN at 24 h. ERalpha may be the primary receptor in the proliferation of HEC-1B cells in response to E(2). Both ERalpha and ERbeta are involved in the agonist impact of TAM on endometrial cancer cells.

Cell Proliferation↗
PubMedDate not supplied

Diabetes in urban African Americans: functional health literacy of municipal hospital outpatients with diabetes.

Functional health literacy was assessed in 63 patients from the diabetes outpatient clinic, 20 from the general medicine clinic, and a total of 48 from two satellite medical clinics. All patients received a demographic questionnaire, visual screening, and the Test of Functional Health Literacy in Adults, an instrument with good validity and internal consistency used to measure the ability to read and understand medical instructions. Functional health literacy was adequate in only 47% of new patients at the diabetes clinic and only 25% of established patients at all sites. There were no significant differences in functional health literacy among established patients across all sites. Overall, patients' mean functional health literacy level was inadequate to marginal. Of the patients with inadequate functional health literacy, 43% denied difficulty in reading. Patient education strategies and materials are needed to address this important barrier to healthcare delivery.

Adult↗
PubMedDate not supplied

Thymidine-analog and multi-nucleoside resistance mutations are observed in both zidovudine-naive and zidovudine-experienced subjects with viremia after treatment with stavudine-containing regimens.

OBJECTIVE: The type and frequency of mutations in the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase coding region observed in virus from antiretroviral therapy (ART)-experienced, zidovudine (ZDV)-naive subjects receiving stavudine (d4T)-based therapies were compared with mutations observed in virus from ART-experienced subjects with previous ZDV exposure. METHODS: Plasma HIV-1 RNA was isolated from 67 ART-experienced subjects. Reverse transcriptase mutations were assessed by sequencing polymerase chain reaction products. RESULTS: Thirty-four subjects (51%) were ZDV-experienced (Z(exp)) and 33 (49%) were ZDV-naive and d4T-experienced (d(exp)Z(naive)). Human immunodeficiency virus type 1 from 16 of 33 (48%) d(exp)Z(naive) subjects and from 16 of 34 (47%) Z(exp) subjects had thymidine analog mutations (TAMs). Multi-nucleoside resistance (MNR) mutations were observed in virus from 5 of 33 (15%) d(exp)Z(naive) subjects and 3 of 34 (9%) Z(exp) subjects. At least one TAM or MNR mutation was identified in 18 of 33 (55%) of the former and in 19 of 34 (56%) of the latter group. CONCLUSIONS: These results confirm recent reports that TAMs and MNR mutations can arise in subjects receiving d4T-based therapy who are naive with respect to ZDV.

Adult↗
PubMedDate not supplied

Differential expression of TRAIL-R3 and TRAIL-R4 in human pancreatic cancer.

BACKGROUND: Pancreatic cancer is one of the most aggressive cancers, in part due to its insensitivity to most treatment modalities. This resistance towards cytotoxic therapy is thought to be caused--at least in part--by a general resistance of pancreatic cancer cells towards apoptosis. TRAIL-R3 and TRAIL-R4, which belong to the TRAIL receptor family, can inhibit TRAIL-induced apoptosis. PATIENTS AND METHODS: Seven normal pancreatic tissues and 7 pancreatic cancer tissues were analyzed using Northern blotting, Western blotting and immunohistochemistry. RESULTS: TRAIL-R3 mRNA and protein expression were generally weak in pancreatic cancers and normal pancreatic tissues. In contrast, TRAIL-R4 mRNA and protein were expressed at moderate to high levels in human pancreatic cancer tissues, but demonstrated weak to negative expression in the normal pancreas. CONCLUSION: TRAIL-R4 but not TRAIL-R3 levels were significantly different in pancreatic cancer in comparison to the normal pancreas. These findings give new insight into the resistance mechanisms of pancreatic cancer towards TRAIL-mediated apoptosis.

Adult↗