Biomedical subjects

Q He

Publications and source records attributed to Q He.

At least 235 records · Page 13Linked to original sources

PubMed1995

[Method of designing a mold for processing corneal lenticules in epikeratophakia with excimer laser].

PURPOSE: To design a mold for processing optical power in epikeratophakia. METHOD: With excimer laser a corneal graft with appropriate optical power shall be got on the formula of the mold's radius curvature. RESULTS: Based upon the formula from the data, a 12D mold was accomplished to obtain a suitable cornea lens for epikeratophakia. CONCLUSION: The 12D mold presents the value to some extent in processing the desired optical power of corneal graft for those patients requiring epikeratophakia.

Epikeratophakia↗

PubMed1994

A physical map of the Myxococcus xanthus chromosome.

A physical map of the 9.2-Mbp Myxococcus xanthus DK1622 chromosome at a resolution of 25 kbp was constructed by using a strategy that is applicable to virtually all microorganisms. Segments of the chromosome were used as hybridization probes to subdivide a yeast artificial chromosome (YAC) library into groups of linked clones. The clones were aligned by comparing their EcoRI restriction patterns. The groups of YAC clones ("contigs") were oriented and aligned with the genomic restriction map by means of common genetic and physical markers such as rare restriction sites and transposon insertions. Over 95% of the genome is represented by cloned DNA. Sixty genetic loci including > 100 genes, many of which play a role in fruiting body development, have been mapped in this way. Additional genes can now be located on the chromosome map by hybridization of their sequences to the ordered set of YAC chromosomes. The mapped genetic loci account for approximately 2% of the genome.

Base Sequence↗

PubMed1994

Effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic DNA analysis.

The effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic DNA analysis were tested. Purified Bordetella pertussis DNA was analysed with four primers (12-mer), which did not differ from each other in their GC-content. Three different thermocycler models from two manufacturers were tested. Three of the primers produced consistent banding patterns in separate wells of the reaction plates of individual thermocyclers, and in different thermocycler models. In contrast, the fourth primer showed extensive between-instrument variation, and the within-instrument variation with this primer was acceptable only when the most advanced thermocycler model was used. We conclude that only primers that provide consistent banding patterns in different environments should be chosen for interlaboratory use in randomly amplified polymorphic DNA analysis. The primers showing variability in banding patterns can only be used if they produce consistent results in the thermocycler used.

Base Sequence↗

PubMed1994

Gene expression of transforming growth factor beta 1 and extracellular matrix proteins in murine Schistosoma mansoni infection.

BACKGROUND/AIMS: The study of Schistosoma-induced hepatic fibrosis in murine Schistosoma mansoni infection has elucidated the nature of hepatic fibrosis in humans. In the present study, fibrogenic gene expression was determined in murine S. mansoni infection during primary infection, after chemotherapy with praziquantel, and during secondary infection. METHODS: Both histomorphometric analysis and Northern blot profiles were performed. RESULTS: Histomorphometric analysis of granulomatous inflammation showed smaller hepatic fibrotic granulomata after chemotherapy and during secondary infection. Albumin gene expression remained relatively constant throughout primary infection, chemotherapy, and secondary infection. Fibronectin gene expression in primary infection was comparable with the level observed in noninfected mice and was reduced by chemotherapy. Reinfection resulted in augmented expression levels equal to primary infection levels. Osteonectin gene expression was active in primary infection, was reduced by chemotherapy, and was actively reexpressed in secondary infection. Interstitial matrix macromolecules, types I and III collagen, and basement membrane collagen showed high levels of gene expression in primary infection, were virtually terminated by chemotherapy, and were reexpressed on reinfection. The gene expression of transforming growth factor beta 1, a major, fibrogenic cytokine, paralleled collagen expression. CONCLUSIONS: Chemotherapy of schistosomiasis initiated a dramatic decrease in steady-state messenger RNA levels of major proteins associated with fibrosis; reinfection resulted in a reexpression of these genes.

Animals↗

PubMed1994

Sensitive and specific polymerase chain reaction assays for detection of Bordetella pertussis in nasopharyngeal specimens.

Polymerase chain reaction (PCR) assays that amplify segments of a repeated gene element and a toxin promoter gene of Bordetella pertussis were compared with a culture established for the diagnosis of pertussis. Of 44 nasopharyngeal (NP) aspirates collected during a pertussis outbreak, repeated gene element PCR showed a positive result in 21 (48%), including all three patients with positive culture results. Results of toxin promoter gene PCR were positive in eight (18%) cases, and the pathogen was not detected in one patient with a positive culture result. A more sensitive nested PCR assay, based on repeated gene element PCR, was then developed. During a second outbreak two different transportation systems were tested in 146 duplicate NP swabs. Transportation of swabs in empty tubes proved to be better than in transport media for PCR. A total of 190 NP specimens from the two outbreaks were tested, and in 56 the results were shown to be positive by PCR, including all 16 cases confirmed as positive by culture. We conclude that the PCR assay is more sensitive than culture in the diagnosis of pertussis; NP swabbing is a simple, practical, and reliable method of collecting clinical specimens for PCR assays and cultures.

Adult↗

PubMed1994

Prediction of the oxygen cost of the deadlift exercise.

The purpose of this study was to examine the relationship between total estimated work, bar load and total oxygen consumption (TVO2) during the deadlift exercise. Forty-two observations of TVO2 during a wide range of deadlifting bouts were made on a heterogeneous sample of 10 males and 14 females. TVO2 was measured by standard open-circuit spirometry. An R of 0.912 for work and TVO2 indicated that total estimated work during deadlifting can be used to predict oxygen cost accurately. The calculated regression equation was TVO2 (litres of O2) = 2.63 + 0.80 work (kJ) with a S.E.E. of 1.50 litres O2. An R of 0.909 for bar load and TVO2 indicated essentially the same predictability using bar load as the independent variable. The calculated regression equation was TVO2 (litres O2) = 2.88 + 0.005 bar load (kg) with a S.E.E. of 1.5 litres O2. Care should be taken when converting oxygen cost to energy expenditure values using non-protein R equivalents, since underestimations are likely, due to the heavy glycolytic involvement.

Adult↗

PubMed1994

Effects of resistance exercise and cycling on recovery blood pressure.

The purpose of this investigation was to examine blood pressure responses during recovery from resistance exercise and cycling. Seven normotensive volunteers (5 males, 2 females) were studied. Resistance exercise consisted of three sets of five exercises (arm curl, hamstring curl, squat, 'lat pull' and bench press) on four occasions: (1) twice using 40% one-repetition maximum (1RM) for 20-25 repetitions, and (2) twice using 70% 1RM for 8-10 repetitions. The subjects also cycled for 25 min at 70% of heart rate reserve on two occasions. Measurements of recovery systolic and diastolic blood pressure were made at 2, 5, 10, 15, 30 and 60 min post-exercise with subjects in the seated position. Analysis of variance with repeated measures revealed that there were no significant differences between recovery blood pressures after the cycling or resistance exercise treatments. Average systolic blood pressure after 2 min of recovery was elevated (115 +/- 2 mmHg, P < 0.0001) and diastolic blood pressure was depressed (48 +/- 3 mmHg, P < 0.0001) in comparison to previously determined baseline values. Systolic blood pressure at 5 min and diastolic blood pressure at 15 min post-exercise were not significantly lower than resting baseline values, and remained so after 60 min of recovery. It was concluded that different intensities of resistance exercise produce the same blood pressure response in recovery and that these changes were approximately equal to those produced by cycling.

Adult↗

PubMed1994

Antibodies to filamentous hemagglutinin of Bordetella pertussis and protection against whooping cough in schoolchildren.

A pertussis outbreak was studied prospectively in an elementary school with 39 pupils. All had been immunized with at least three doses of Finnish diphtheria-tetanus toxoid-pertussis vaccine. Diagnosis of pertussis was based on culture, polymerase chain reaction results, and EIA serology using filamentous hemagglutinin (FHA), pertussis toxin, and 69-kDa outer membrane protein as antigens. At the first sampling, 21 children had symptoms suggestive of pertussis, and 18 were healthy. Of the latter, 8 remained healthy without any antibiotic treatment and 9 developed clinical pertussis 1-22 days later. One child developed cough later, but this symptom did not meet criteria for pertussis. The mean levels of IgG, IgM, and IgA antibodies to FHA were significantly higher in 8 healthy children than in 9 children who developed pertussis after the first sampling (P < .001, P = .027, and P = .011, respectively). The results show that antibodies to FHA of Bordetella pertussis in immunized schoolchildren correlate with protection against pertussis.

Adhesins, Bacterial↗

PubMed1994

Outcomes of Bordetella pertussis infection in different age groups of an immunized population.

Outcomes of Bordetella pertussis infection were studied in 3 age groups (1-3, 4-6, and 7-15 years) during outbreaks in one day care center (n = 29) and in two elementary schools (n = 210). A total of 76 children were confirmed as having B. pertussis infection; 74 were confirmed by polymerase chain reaction (PCR) and 18 by culture. A positive PCR result was less common in children 1-3 years old than in those 4-6 (P = .006). Asymptomatic B. pertussis infection was more common in preschool children than in schoolchildren (P < .001), whereas schoolchildren presented with symptoms compatible with clinical pertussis more often than preschool children (60/210 vs. 3/29, P = .029). In the community of 12,691, the attack rate (laboratory-confirmed patients/100,000) was 317 in children < 4 years, 1838 in children 4-6 years, 2535 in children 7-15 years, and 248 in persons > 15 years. The protection provided by conventional pertussis vaccine is evidently rather short-lived, and booster vaccinations are needed to eradicate pertussis among schoolchildren and adults.

Age Factors↗

PubMed1994

Putative anticryptosporidial agents tested with an immunodeficient mouse model.

Lasalocid, sinefungin, and dehydroepiandrosterone were tested for anticryptosporidial activity with an immunodeficient mouse model at doses that have been reported effective when tested with immunosuppressed rodent models. Small but significant reductions in oocyst excretion were only observed under some conditions with lasalocid and dehydroepiandrosterone, but sinefungin had no effect.

Adenosine↗

PubMed1994

Arginine-derived nitric oxide reduces fecal oocyst shedding in nude mice infected with Cryptosporidium parvum.

Dietary L-arginine (4%) significantly reduced fecal oocyst shedding in athymic nude mice chronically infected with Cryptosporidium parvum. This effect appeared to be due to an increase in host nitric oxide (NO) production as it was not observed in arginine-supplemented animals administered the NO synthase inhibitor, N-nitro-L-arginine methyl ester. N-Nitro-L-arginine methyl ester alone significantly increased fecal oocyst shedding in chronically infected animals. In in vitro assays, oocyst excystation and sporozoite viability were significantly reduced by the NO donors sodium nitroprusside and S-nitroso-L-acetyl penicillamine in a concentration-dependent manner. These data suggest that arginine-derived NO may reduce the parasite load in experimental cryptosporidiosis.

Amino Acid Oxidoreductases↗

PubMed1994

Typing of group A streptococci by random amplified polymorphic DNA analysis.

Random amplified polymorphic DNA (RAPD) analysis was evaluated in comparison with restriction endonuclease analysis (REA) of genomic DNA and serotyping in the typing of 160 epidemiologically unrelated group A streptococci (GAS). Amplification of genomic DNA of GAS was performed with a single primer with an arbitrarily selected nucleotide sequence of 12 nucleotides. In total, 31 RAPD patterns and 15 REA patterns were observed among the isolates studied. The results of RAPD analysis were in accordance with the results of REA for 86% of the isolates, as both methods identified 15 different strains among 138 isolates. However, RAPD analysis differentiated 16 additional strains among 22 isolates. RAPD analysis was somewhat better than REA for differentiation of isolates of the same and different serotypes. However, not all of the serotypes were differentiated by RAPD analysis either. In conclusion, RAPD analysis provides a practical alternative for genomic typing of GAS. It can be recommended for the typing of GAS, especially if used in parallel with serotyping.

Bacterial Typing Techniques↗

PubMed1994

Monoclonal antibodies against the Fc fragment of IgA alpha-chain: preparation and clinical application.

Fragments of the heavy alpha-chain of IgA (IgA Fc alpha) were prepared and purified from the serum of patients with heavy chain disease. It was used to immunize BALB/c mice. The immunized mouse spleen cells were then fused with myeloma cells Sp2/O. Through successive recloning of the hybridoma cell lines by the limiting dilution method, four subclonal hybridoma cells (D2, E4, F5 and G10) capable of screening IgA Fc alpha were found. Intraperitoneal transplantation of these cells induced ascites in the BALB/c mice, from which four types of monoclonal antibodies (McAb-IgA Fc alpha) were obtained. The highest McAb titer was as high as 1:16348 by the hemagglutination inhibition (HAI) test. These McAb's reacted specifically with IgA Fc alpha, but not with IgG, IgM, IgD, kappa or lambda. Serum samples from 92 normal subjects and 73 patients with various diseases were tested for Ig genetic markers by means of HAI. The family trees of three positive cases were surveyed. Results showed that the McAb-IgA Fc alpha may be used to determine the genetic marker of IgA allotypes.

Adult↗

PubMed1994

[Study on urine ALb, serum and urine beta 2-m, IgG, and THP].

The urine ALb, serum and urine beta 2-m, IgG, and THP were serially determined with radioimmunoassay to assess the renal functions during various stages of burn in 52 patients with TBSA over 30% (mean +/- s 58.4% +/- 18.88%). It was found that the urine levels of ALb, IgG, and THP and the serum and urine levels of beta 2-m were remarkably increased and that the serum levels of IgG were decreased after burns. Our study suggested that the measurements of urine ALb, serum and urine beta 2-m, IgG, and THP using radioimmunoassay were sensitive in assessing renal glomerular permeability and tubular functions after burn. It is presumed that such methods might be used to help identify the location, the extent, and the severity of the renal abnormalities and provide valuable information about the differential diagnosis and the prognosis of the renal damage in patients with extensive burns.

Adolescent↗

PubMed1994

[Mapping of face and extremity skin cleavage lines in Chinese adult cadavers].

On fresh Chinese adult cadavers, which were obtained within 6 hours of sudden death, multiple skin holes of 1 cm apart were made with a conical steel punch of a diameter of 5 mm. Chinese ink was dipped into the wounds, which presently appeared to be rhomboid slits. Mapping of the directions of these rhomboid slits gave rise to cleavage lines of that area. This paper presents the skin cleavage lines of the face and extremities in Chinese adult cadavers.

Adult↗

PubMed1994

[Villous tumor of the duodenum].

8 cases of duodenal villous tumor were reported. A total of 11 tumors, with 2 in the bulb and 9 in the descending part, were excised. Malignant change was found in 4 cases (50%). Diagnosis could be made by using hypotonic duodenography and fibroscopy. The integral tumor should be examined by frozen section during operation, so as to determining the appropriate operation for satisfactory result. Postoperative follow up should be done periodically because of the possibility of recurrence after local excision.

Adenoma, Villous↗

PubMed1994

[Repair of soft tissue defects of hand with pudendo-femoral skin flaps].

Soft tissue defects of the hand have been repaired with free pudendo-femoral skin flaps in 13 patients. The results are quite satisfactory in all cases. The flaps are thin with little fat to interfere with post-operative appearance. The donor sites are well-hidden and can be primarily closed with no functional disturbances. Hence the ideal donor site for soft tissue defects of the hand.

Adolescent↗

PubMed1994

Primers are decisive for sensitivity of PCR.

A sufficient sensitivity of PCR is a prerequisite for its use in the diagnosis of infectious diseases. We have used PCR for detecting gene elements of Borrelia burgdorferi, mycobacteria and Bordetella pertussis. With all these microbe groups, difficulties were encountered in achieving the demanded sensitivity with the primer pairs primarily selected. An extensive testing of various reaction parameters did not improve the sensitivity. Subsequently, we synthesized more primers derived from slightly different positions of the original target sequences. When the original and new primers were tested in possible combinations, some primer pairs reached 100-fold to 1000-fold higher sensitivity than the primary pairs. We conclude that in optimizing the sensitivity of PCR, more emphasis should be put on testing of several primer pairs than on the extensive screening of reaction parameters. Thus far, a trial-and-error approach has to be used, because there is no means to predict the sensitivity properties of a selected primer pair.

Base Sequence↗