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Ping-Fang Tian

Publications and source records attributed to Ping-Fang Tian.

2 recordsLinked to original sources

Progress in plant CACTA elements.

Transposable elements are DNA fragments that can insert new chromosomal locations. On the basis of the mechanism of transposition, transposable elements were divided into two classes. Class 1 elements were retroelements that used reverse transposase to transpose by an RNA intermediate. Class 2 elements or DNA transposons transposed directly from DNA to DNA. Of the Class 2 elements, CACTA superfamily, so far identified exclusively in plants and previously regarded as low-copy-transposon for the conserved mechanism of propagation, recently received considerable interest because of their increasing evidence reiterating their high copies in some plant genomes. This article aimed at outlining CACTA elements with regard to their structure, transposition, and utilization.

DNA Transposable Elements↗

[The influence of soybean isoflavone on expression of low density lipoprotein receptor (LDLR) mRNA in ovariectomied rats].

OBJECTIVE: To investigate the effect of soybean isoflavone on blood lipid and the expression of LDLR mRNA in ovariectomied rats. METHODS: Forty eight Sprague-Dawley rats were divided randomly into four groups: Sham group (sham), OVX group (OVX), OVX + gemifibrozil (G) group (OVX + G) and OVX + isoflavone (ISO) group (OVX_ISO). in which the rats were treated with G or ISO for three months starting from two weeks after both sides of rat' s ovarietomy. Blood sample were taken out for determination of blood lipid. The liver tissue were taken out quickly, Isothiocyanate guanidine-phenol-chloroform was used to extract the total RNA from the liver and RT-PCR was used to detect the expression of LDLR mRNA. RESULTS: Compared with the OVX group, contents of TG, LDL-C and OX-LDL in serum in OVX + ISO group decreased remarkably, and the contents of HDL-C increased. LDLR mRNA in OVX + ISO group increased distinctively compared with OVX group and OVX + G group. CONCLUSION: The level of mRNA of LDLR in liver decreas after ovariotormy and the soybean isoflavone may increase it by regulating the expression level of LDLR through transcription.

Animals↗