Biomedical subjects
Publications and source records attributed to Patrick Tabeling.
Experiments performed on droplets breaking up in microfluidic junctions of various angles are described. A critical length is found that controls the breakup process. This quantity depends on the flow geometry only; it is independent of the flow conditions and the fluid characteristics. A theory assuming small capillary numbers describes well the conditions that govern the breakup process.
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A microfluidic poly(dimethylsiloxane) (PDMS) microdevice was realized, combining on-line protein electrophoretic separation, selection, and digestion of a protein of interest for identification by mass spectrometry. The system includes eight integrated valves and one micropump dedicated to control the flow operations. Myoglobin was successfully isolated from bovine serum albumin (BSA), then selected using integrated valves and digested in a rotary micromixer. Proteolytic peptides were recovered from the micromixer for protein identification. Total analysis from sample injection to protein identification is performed under 30 minutes, with samples of tens of nanolitres. The paper shows that PDMS technology can be successfully used for integrating complex preparation protocols of proteic samples prior to MS analysis.
We describe a method based on plasma polymerization for the modification and control of the surface properties of poly(dimethylsiloxane) (PDMS) surfaces. By depositing plasma polymerized acrylic acid coatings on PDMS, we succeeded to fabricate stable (several days) hydrophilic and patterned hydrophobic/hydrophilic surfaces. We used this approach to generate direct and (for the first time in this material) double emulsions in PDMS microchannels.
We use pervaporation-based microfluidic devices to concentrate species in aqueous solutions with spatial and temporal control of the process. Using experiments and modeling, we quantitatively describe the advection-diffusion behavior of the concentration field of various solutions (electrolytes, colloids, etc.) and demonstrate the potential of these devices as universal tools for the kinetic exploration of the phases and textures that form upon concentration.
A theoretical model is proposed to extract rate constants of second-order chemical reactions down to the millisecond time scale from the observation of reaction-diffusion processes in a microchannel. We validate this theoretical approach by examining an appropriate model reaction. The measured rate constant is in excellent agreement with this obtained from nuclear magnetic resonance experiments.
We measure velocity profiles in water flowing through thin microchannels, using particle image velocimetry combined with a nanopositioning system. From the velocity profiles, we determine the slip lengths in two cases: Smooth hydrophilic glass surfaces, and smooth hydrophobic glass surfaces, grafted with a monolayer of silane. The slip length is determined within (+/-100 nm) , i.e., five times more accurately than previous work. In all cases, we find that the slip length is below 100 nm.
We confront, quantitatively, the theoretical description of the reaction-diffusion process of a second-order reaction to experiment. The reaction at work is Ca(2+)/CaGreen, a fluorescent tracer for calcium. The reactor is a T-shaped microchannel, 10 microm deep, 200 microm wide, and 2 cm long. The experimental measurements are compared with the two-dimensional numerical simulation of the reaction-diffusion equations. We find good agreement between theory and experiment. From this study, one may propose a method of measurement of various quantities, such as the kinetic rate of the reaction, in conditions yet inaccessible to conventional methods.
We show that wetting properties crucially control the patterns in two-phase flows of immiscible fluids in microchannels. Ordered patterns, continuously entrained by the flow, are obtained when one phase completely wets the walls, while disordered patterns, intermittently adhering to the channel walls, are unavoidably produced when wetting is partial. A lower limit for the channel sizes capable of generating well structured objects (drops, pears, pearl necklaces, ...) is presented.
Among the available diagnostics of turbulence, the flatness of the velocity derivatives is particularly interesting because it represents a straightforward test of Kolmogorov theory, and provides a quantitative estimate for intermittency effects. It is commonly considered that the flatness factor increases with the Reynolds number, following a power law at high Reynolds numbers. At variance with this picture, evidence for a transitional behavior, taking place around the Taylor microscale Reynolds number R(lambda)=700, has been recently obtained in several experiments. In the present paper we study this transition in detail, and show it has the characteristics of a second order phase transition. We propose a physical picture for this transition, based on worm vortex breakdown, which leads as to suggest that intense sub-Kolmogorov structures might develop above the transition point. These results indicate that the existence of an asymptotic state at infinite Reynolds number may become questionable and more generally, that our current views on dissipative range intermittency probably need to be revised