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Biomedical subjects

P Wilding

Publications and source records attributed to P Wilding.

At least 73 records · Page 4Linked to original sources
PubMed1976

Factors involved in the determination of triglycerides in serum: an international study.

Ten laboratories analysed five different specimens in duplicate on ten separate occasions by one, or several, of five common triglyceride methods. Simple statistical data are presented and, as far as possible, these are interpreted in the light of the methods used and the results of chemical analyses of the materials. Great variability was found between the results of the participating laboratories. The major factor involved seems to be the material specific nature of the methods under study. At least one method in common use is contraindicated.

Chromatography, Gas↗
PubMed1976

The application of thin-layer gel filtration chromatography in the rapid assessment of protein-protein coupling reactions.

The use of enzymes as markers when covalently coupled to various antigens or antibodies has wide application in medical science. A variety of bifunctional reagents has been used to produced the conjugates, and there have been some attempts to investigate the conditions of reaction necessary to obtain good coupling, whilst preserving the biological function of the molecules. The present study describes the influence of coupling conditions on the coupling of a alpha-amylase and albumin by a range of commercially available bifunctional coupling reagents, and the application of thin-layer gel filtration chromatography for the rapid qualitative/semi-quantitative assessment of coupling reactions.

Albumins↗
PubMed1976

Evaluation of a new enzymatic kit for serum triglycerides.

We evaluated a new enzymatic kit for serum triglycerides (triacylglycerols) in two laboratories. The procedure involved both enzymatic hydrolysis of the triglycerides and conversion of the glycerol, which was linked to an indicator reaction. There was excellent agreement with the mechanized procedure of Kessler and Lederer and with a manual method. The test requires 50 mul of serum and can be completed in about 50 min. Inter- and intra-laboratory variation, linearity, accuracy, and specimen storage were studied.

Autoanalysis↗
PubMed1975

Letter: SI units.

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Clinical Laboratory Techniques↗
PubMed1975

The influence of drugs and disease activity on biochemical and haematological data in rheumatoid arthritis.

Biochemical and haematological data from 218 patients with rheumatoid arthritis were analysed and compared with data from a reference hospital population. The comparison demonstrated significant differences in several biochemical and haematological tests and that the patterns of change are different between males and females. The data were also analysed by conventional statistical methods and discriminant analysis using a computer to establish which tests were most influenced by the activity of the disease and drug therapy. The results obtained demonstrate marked difference between groups of patients with different disease activity or receiving different drugs. The discriminant analysis also identified those tests which differentiate these groups most effectively.

Arthritis, Rheumatoid↗
PubMed1975

Letter: SI units.

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International Cooperation↗
PubMed1975

Effect of paracetamol on uric acid determination.

The effect of paracetamol on uric acid determination using a phosphotungstate reduction method has been investigated. The results show that apparently raised values of serum uric acid may be reported in patients receiving therapeutic levels of this drug. Levels of serum uric acid were determined over a five-hour period in three patients who had received therapeutic doses of paracetamol. The assays of serum uric acid were performed by a method based on phosphotungstate reduction and by one employing uricase. The results illustrate the problems of using the former method for clinical purposes.

Acetaminophen↗
PubMed1975

An evaluation of the Greiner Selective Analyzer and its role in the clinical laboratory.

The Greiner Selective Analyzer (GSA II) was evaluated over a period of six months. The evaluation assessed the reliability, accuracy, and precision of the analyser for six determinations. The methods evaluated were for glucose, urea, creatinine, total protein, total bilirubin, and alkaline phosphatase. Comparison of results was also made with those obtained for the same specimens using the Technicon SMA 12160 Analyzer. Correlation and comparison of results indicate that the Greiner Selective Analyzer performed better for three of the methods but worse for serum creatinine determination. The role of the analyser as a routine tool in t heclinical laboratory was also evaluated during analyses of approximately 900 patient specimens. Other features evaluated were analytical range of the six methods under study, the economics of operation, temperature control, and electrical and mechanical safety. Additional key phrases: Accuracy, carryover, economics of operation, analytical ranges, reliability, precision.

Alkaline Phosphatase↗