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P Verdugo

Publications and source records attributed to P Verdugo.

45 records · Page 3Linked to original sources
PubMed1977

Molecular arrangement of cervical mucus: a reevaluation based on laser light-scattering spectroscopy.

Evidence obtained from laser light-scattering spectroscopy suggests that the molecular arrangement of cow estrous cervical mucus is an ensemble of entangled random-coiled macromolecules rather than a cross-linked macromolecular network, the model heretofore widely accepted. This new model can account for the following phenomena: (1) The viscoelastic properties of the estrous mucus and human mid-cycle cervical mucus are the result of the way in which the glycoproteins are entangled. Variations of these properties during the reproductive cycle may simply be due to changes in the water content of the cervical mucus. (2) Penetration of spermatozoa in mucus may be entirely mechanical without involving any kind of enzymatic lysis of cross-links (this is supported by changes in the pattern of spermatozoa flagellation during sperm penetration of cervical mucus as shown in motion picture films). (3) The orientation of spermatozoa in the cervical muscus need not be due to the presence of 'channels' between micelles but simply to an artifact, the stretching or flow of the mucus, which orients the entangled macromolecules and thus facilitates the penetration of spermatozoa in an axis parallel to them.

Animals↗
PubMed1976

Laser light-scattering spectroscopy: a new application in the study of ciliary activity.

A uniquely precise and simple method to study ciliary activity by laser light-scattering spectroscopy has been developed and validated. A concurrent study of the effect of Ca2+ on ciliary activity in vitro by laser scattering spectroscopy and high speed cinematography has demonstrated that this new method is simpler and as accurate and reproducible as the high speed film technique.

Cilia↗
PubMed1975

Kinetic characteristics of the excitability-inducing material channel in oxidized cholesterol and brain lipid bilayer membranes.

The kinetic characteristics of the opening and closing of the excitability-inducing material (EIM) channel in oxidized cholesterol and in brain lipid bilayers are compared. The kinetics of the opening and closing of individual ion-conducting channels in bilayers doped with small amounts of EIM are determined from discrete fluctuations in ionic current. The kinetics for approach to steady-state conductance are determined for lipid bilayers containing many channels. Steady-state and kinetic characteristics for the EIM channel incorporated in brain lipid bilayers can be accounted for by the model developed for the EIM channel incorporated in oxidized cholesterol membranes. Relaxation time, calculated from rate constants of single-channel membranes or directly measured in many-channel membranes is strongly temperature dependent, and is always shorter in brain lipid membranes. Changes in temperature do not affect the interaction of the electric field and the open channel, but the open configuration of the EIM channel in brain lipid bilayers is stablized with increasing temperature. The configurational energy difference between the open and closed channel, calculated from temperature studies, is larger in brain lipid bilayers. The energy barrier which separates the two configurations of the channel is larger in oxidized cholesterol bilayers.

Alamethicin↗
PubMed1975

Methods for studying oviductal physiology.

In studying oviductal physiology, it is important to sort out the complex interrelationships between muscle, cilia, nerves and secretory processes as they each of themselves, or in concert, effect gamete transport. In this review, a variety of physiological techniques and bioengineering approaches which have been used to monitor contractile and ciliary activity, are described and critically evaluated.

Animals↗
PubMedDate not supplied

Characterization of a marker for tracheal basal cells.

An IgM monoclonal antibody (1D9/B3) is characterized, which specifically recognizes basal cells of the upper airway epithelium. Although morphological features have been used to follow cell lineage and differentiation, an objective assessment of differentiation can be enhanced by characterizing the expression of specific antigens that form the phenotypic profile of specialized cells. Mice were immunized with rabbit tracheal basal cells that had been obtained by pronase digestion and purified into a subpopulation of basal cells by flow cytometry. Six immunization experiments produced five hybridomas specific to epithelial cells. A hybridoma whose supernatant immunocytochemically stained the basal cell subpopulation of rabbit tracheal cells was selected. The antibody reacted with tracheal basal cells in rabbit, rat, sheep, pig, and human tracheal sections, and in cultured monolayers of tracheal epithelial cells of the same species. The antibody did not react with the basal cells of other rabbit tissue, including the skin, or other rabbit epithelia. Confocal microscopy and exposure of tracheal epithelial cells to fluorescent-tagged monoclonal antibody 1D9/B3 prior to loading on to flow cytometry showed that the basal cell antibody recognized an intracellular epitope. The epitope for the 1D9/B3 antibody was characterized by Western blotting. The 1D9/B3 antibody appears to be a distinct and specific marker to the airway epithelial basal cell and will be useful in studies of airway epithelial differentiation, injury, and regeneration.

Animals↗