Biomedical subjects
P Valenzuela
Publications and source records attributed to P Valenzuela.
Fertility control with norgestrel and ethynil estradiol. Report on continuing studies.
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Kinetic properties of succinylated and ethylenediamine-amidated -chymotrypsins.
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Acylation of - and -chymotrypsins by p-nitrophenyl acetate. Enzyme-substrate complex formation and pH dependence.
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The difference between alpha-and delta-chymotrypsins. Preparation and alkaline pH dependence of alpha1-chymotrypsin-catalyzed hydrolysis of N-acetyl-L-tryptophan methyl ester (ATME). The involement of alanine-149 in alpha-chymotrypsin catalysis.
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Conformation of delta-chymotrypsin during catalysis. Chemical reactivity of the isoleucine 16 amino group in the acyl-enzyme intermediate.
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Binding of competitive inhibitors to delta-chymotrypsin in the alkaline pH region. Competitive inhibition kinetics and proton-uptake measurements.
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Terpene biosynthesis: formation of nerol, geraniol, and other prenols by an enzyme system from Pinus radiata seedlings.
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Alkaline pH dependence of delta-chymotrypsin-catalyzed hydrolysis of specific substrates.
K(m) (app) and k(cat) for the deltachymotrypsin-catalyzed hydrolysis of N-acetyl-L-tryptophan methyl ester and N-furylacryloyl-L-tryptophanamide were measured as a function of pH and ionic strength. The K(m) (app) values do not increase considerably above pH 9 for delta-chymotrypsin, as is the case with alpha-chymotrypsin. The observed kinetic difference between both enzymes at high pH suggests that the reversible inactivation of alpha-chymotrypsin at alkaline pH may involve the participation of tyrosine 146 or alanine 149 since both residues are present as chain termini in alpha-chymotrypsin but not in delta-chymotrypsin.
Diagnosis of genital amebiasis in women by the standard Papanicolaou technique.
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Phosphorylated intermediates of terpene biosynthesis in Pinus radiata.
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Molecular cloning and physical map of bacteriophage PM2 DNA.
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[Usefulness of antituberculosis drug resistance studies].
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[Gastric perforation in newborn infants].
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[Acute bacterial laryngotracheobronchitis].
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[Creep in amalgams with high copper content].
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[Manipulation and resistance in amalgams with high copper content].
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