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Biomedical subjects

P Vago

Publications and source records attributed to P Vago.

44 records · Page 3Linked to original sources

Is ventilatory anaerobic threshold a good index of endurance capacity?

This study was undertaken to assess whether ventilatory anaerobic threshold (T vent) reflected endurance capacity (EC) in sports medical control. Fifteen subjects performed two cycle ergometer tests. The first was a maximal exercise test, which consisted of increasing the load 20 W/min until exhaustion. During this test, the gas exchange anaerobic threshold was determined and VO2 max was measured. The second was an endurance exercise test, which consisted of asking the subject to work, as long as possible, a load representing 80% of his maximal aerobic power. During this test, we measured endurance time (ET). The statistical analysis showed the lack of relationship between VO2 max and ET and the linear correlations between VO2 T vent ml/min/kg and ET min (r = 0.521, P less than 0.05), VO2 T vent l/min and ET min (r = 0.524, P less than 0.05), and T vent % VO2 max and ET (r = 0.738, P less than 0.01). These results establish that ventilatory anaerobic threshold actually reflects endurance capacity. This relation can be explained referring to the muscle energetic metabolism during exercise. Therefore, T vent should be determined systematically in addition to VO2 max during maximal exercise tests to better evaluate physical fitness.

Adolescent↗

[Cytometric analysis of the DNA content of the thymus, spleen and liver in C57BL/6 mice].

Flow cytometric analysis of cell DNA content in the mouse C 57 BL/6 shows better results with fine needle aspiration than with mechanical dissociation of the tissues. There is no difference fresh and deep frozen stored tissues. In the liver, it has been found two cell populations with different DNA content including diploid and tetraploid cell lines. In the spleen and the thymus, only diploid cells are encountered with different cell cycles belonging to the organ.

Animals↗

[Cytometric study of the effects of destruxin E on leukemic cells in mice].

The activity of destruxin E, a cryptogamic toxin isolated from the hyphomycete Metarhizium anisopliae, was studied on mouse leukemia cells (L 1210 and P 388) in culture. Besides a cytotoxic effect, a cytostatic effect (increase of diploïd cells proportion) was observed with all doses for P 388 (from 10 micrograms/ml to 0.001 microgram/ml) and to a concentration of 0.1 microgram/ml for L 1210. At the lower doses, the effect on cellular DNA content appears distinctly.

Animals↗

[The chronobiology of inflammation].

The inflammatory reaction is characterized by a multifrequency time structure with prominent circadian, ultradian and infradian rhythms in cell proliferation and cell function. The circulating blood cells show highly reproducible circadian rhythms in human and in animals. Daily fluctuations of concentrations of haematologic chemical and endocrinological parameters are described. Circannual variations of several hematologic parameters are presented. Chronobiological studies of plasma exudation in carrageenan paw oedema show a circadian and a circannual rhythm. Daily and annual changes in migration of polymorphonuclear leucocyte produced by BCG are studied in mice. Differences in response are observed in adrenalectomized and castrated animals. The susceptibility of cells and tissues at different stages of their circadian or circannual rhythms lead to the development of a chronotherapy of inflammation.

Animals↗

Breathing pattern and occlusion pressure during exercise in pre- and peripubertal swimmers.

In two groups of young swimmers (prepubertal stage: group A; peripubertal stage: group B), the ventilatory response to graded exercise work with a cycle ergometer was studied. Ventilatory variables (ventilation, VE, tidal volume, VT, respiratory frequency,f, ratio between inspiratory period and total breath duration, TI/TTOT, and mean inspiratory flow, VT/TI) as well as mouth occlusion pressure measured at 100 msec (P0.1), effective impedance of the respiratory system (P0.1/VT/TI), inspiratory power for breathing (W) and O2 uptake (VO2) were measured during the third minute of each work load. At the same level of exercise both groups showed identical values of VT/TI, but VE was higher in group A individuals. This resulted from higher values of respiratory frequency with higher TI/TTOT ratios. P0.1, P0.1(VT/TI) and W were also much higher during work load in group A than in peripubertal subjects. When the above results were related to the same percentage of VO2 max, P0.1, W, respiratory frequency and duty cycle did not differ within both groups. However, VE, VT and VT/TI were lower in group A subjects with a higher P0.1/(VT/TI) ratio. Further corrections of VT, VT/TI and P0.1/(VT/TI) ratios by body weight cancelled all these differences. In conclusion, our results strongly suggest that biometric factors only determined interindividual differences in ventilatory response to exercise in prepubertal and peripubertal swimmers.

Adolescent↗

[Optimization of the method of progressive hypercapnia by determination of the sensitivity threshold].

Ventilatory response to CO2 rebreathing is a method which allows to evaluate the reactivity of chemoreceptors. However this method doesn't study the sensibility threshold, i.e. the Pe.t.CO2 value for which the ventilatory response appears clearly. This sensibility threshold was measured in 10 healthy subjects by rebreathing a gas mixture: 7% CO2 and 50% O2 to avoid hypoxy. It was defined as the value of Pe.t.CO2 for which the ventilation was above the tidal ventilation + 2 standard deviations. The sensibility threshold (51 +/- 4.35 mm Hg) was independent of the reactivity slope represented by the slope of the linear relation between minute ventilation (VE) and Pe.t.CO2 (1.34 +/- 0.60 l/min/mm Hg/m2) and consequently appears as an interesting parameter in order to evaluate the ventilatory response to CO2 by rebreathing.

Adult↗

Morphological and molecular changes in the inner hair cell region of the rat cochlea after amikacin treatment.

This study investigates the morphological and molecular changes that occur in the inner hair cell area of the rat cochlea following aminoglycoside treatment. Rats were injected daily with 500 mg/kg of amikacin between postnatal day 9 (PND9) and PND16. Cochleae were examined at PND16 to PND120 using both scanning and transmission electron microscopy and molecular fluorescent labeling. The inner hair cells showed obvious signs of apoptosis in response to amikacin treatment and most of them were missing by one week after the end of the aminoglycoside exposure period. Concomitantly, the epithelium became scarred as the surrounding supporting cells expanded and filled the space vacated by the missing IHCs. The mid-basolateral region of these modified supporting cells was surrounded by many afferent and efferent terminals. However, these cells expressed neither calbindin nor SNAP25, proteins that are both expressed by IHCs in the normal, untreated organ of Corti in the rat. In addition, these supporting cells remained attached to the basal lamina by a thin cytoplasmic process. The supporting cells surrounding the inner hair cells therefore appear unable to convert directly into inner hair cells following aminoglycoside induced hair-cell loss but may be able to provide trophic support for the remaining afferent and efferent neurites.

Actins↗

Tumor cell membrane as a potential target for methyl-beta-cyclodextrin.

The purpose of this work was to determine the role of methyl-beta-cyclodextrin (MEBCD) in combination with doxorubicin (DOX) on DOX intracellular accumulation and efflux, in comparison to verapamil in a sensitive parental and multidrug-resistant human cancer cell line (HL-60 S and HL-60 R). Moreover, cell membrane and nuclear modifications induced by MEBCD were investigated. At concentration of 10 mumol for 10(6) cells, MEBCD combined with doxorubicin (DOX), was able to significantly enhance the intracellular concentration of DOX in HL-60 S and HL-60 R cell lines during the period of exposure. In the resistant subline, MEBCD activity was higher than that of verapamil. Moreover, treatment of cells with MEBCD resulted in a modification in cell membrane integrity and cell morphology, but had no own activity in the distribution of the cells within cell cycle.

Biological Transport↗