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Biomedical subjects

P Thuvasethakul

Publications and source records attributed to P Thuvasethakul.

17 recordsLinked to original sources
PubMed2000

Nutrients and hormones in heat-dried human placenta.

This cross-sectional study was to assess the nutrients in terms of protein, fat, minerals, and hormones in heat-dried human placenta. Thirty heat-dried human placentas, 15 from male and 15 from female, were analyzed for protein (amino acids), fiber, fat, moisture, minerals (sodium, potassium, phosphorus, calcium, iron, magnesium, zinc, copper, manganese), hormones (estradiol, progesterone, testosterone, growth hormone). Heat-dried female human placentas had slightly higher fiber content than male, but protein and fat components were not different. Mineral levels in placentas were high especially sodium, potassium and phosphorus. There were no significant differences in the amount of minerals and hormonal profile between female and male placentas. However, hormone levels in heat-dried placenta were low compared to physiologic level in human beings. The results of this study suggest that the amount of nutrients particularly protein and minerals in heat-dried human placentas were enriched.

Amino Acids↗
PubMed1999

Congenital pseudarthrosis of the forearm treated by free vascularized fibular graft: a report of three cases and a review of the literature.

Congenital pseudarthrosis of the forearm is a rare condition; approximately 60 cases have been reported in the English literature. We report 3 patients treated by wide excision of the pseudarthrosis and free vascularized fibular grafting. The pseudarthrosis involved the radius in 1 patient and the ulna in 2. Neurofibromatosis was present in 2 patients. The technical problems encountered during the procedures included preservation of the distal epiphysis and attaining stable fixation of the fibular graft without damaging its vascular supply. A review of 15 previously reported patients treated by free vascularized fibular grafting showed that this procedure is excellent in the treatment of congenital pseudarthrosis of the forearm and that the result is consistently reproducible.

Child, Preschool↗
PubMed1999

Five-year thyrotropin screening for congenital hypothyroidism in Ramathibodi Hospital.

OBJECTIVES: To detect newborns with congenital hypothyroidism (CH) and to treat the affected infants as early as possible. STUDY DESIGN: Cord blood thyrotropin (TSH) screening for CH in Ramathibodi Hospital began in 1993. From October 1993 to December 1998, 35,390 neonates were screened. The infants with elevated TSH level of greater than 30 mU/L were recalled for verification of CH. Confirmation tests included total thyroxine, free thyroxine and TSH level. Thyroid scan and uptake were performed in some affected infants. RESULTS: Twelve infants with CH were detected resulting in an incidence of one in 2,949 live-births. All affected infants were asymptomatic at birth. Of 12 infants with CH, one premature neonate had a delayed TSH elevation and was diagnosed as having primary hypothyroidism at 2 months of age. The recall rate for validation of CH based on a cut-off value at serum TSH level of greater than 30 mU/L is 1.1 per cent. If the cut-off value of serum TSH level was raised to greater than 40 mU/L, the recall rate would decrease to 0.43 per cent. None of the affected infants had cord blood TSH level of less than 50 mU/L except one premature patient. Therefore, beginning in January 1997, the cut-off value of TSH was raised to 40 mU/L or greater. Pitfalls in this program include incomplete blood-specimen collection and incomplete follow-up. To strengthen the program, improvements were made in the follow-up system from 1996 onward. Therefore, the coverage for blood-specimen collection progressively increased from 84 per cent in 1994 to 96 per cent in 1998. Simultaneously, the patients' return after recalls also increased from 38 per cent to 100 per cent. CONCLUSIONS: The incidence of CH in Ramathibodi Hospital is approximately 1:3,000 live-births. The optimal cord blood TSH level for recall is 40 mU/L or greater. The intensification of follow-up strategy resulted in better response to recall and earlier treatment in the affected infants.

Congenital Hypothyroidism↗
PubMed1998

Nerve transfer to biceps muscle using a part of the ulnar nerve in brachial plexus injury (upper arm type): a report of 32 cases.

Thirty-two patients with absent elbow flexion secondary to brachial plexus injury underwent nerve transfer using 1 or 2 fascicles of the ulnar nerve to the motor branch of the biceps muscle. Twenty-six patients had root avulsion injury of C5 and C6; 4 had root avulsion injury of C5, C6, and C7; and 2 had lateral and posterior cord injury with distal injury of the musculocutaneous nerve. The follow-up period ranged from 11 to 40 months (average, 18 months). Thirty patients had biceps strength of M4 (flexion power ranged from 0.5 to 7 kg) and 1 had biceps strength of M3. All but 1 patient demonstrated signs of recovery of the biceps muscle. No notable impairment of hand function was observed.

Adult↗
PubMed1998

Anatomy and internal topography of the musculocutaneous nerve: the nerves to the biceps and brachialis muscle.

One hundred twelve musculocutaneous nerves from 56 cadavers were examined to determine branching patterns of innervation and internal neurotopography. There were 3 distinct types of branching patterns for biceps innervation: in 62%, there was 1 branch only; in 33%, 2 branches; and in 5%, 3 branches. The origin of the first branch averaged 130 mm from the acromion regardless of branching type. The maximum distance between the first and second branch was 53 mm. In 92%, there was only 1 branch to the brachialis muscle. It always emerged from the main trunk distal to the nerve to the biceps and averaged 170 mm from the acromion. Internal topography was studied from 1-mm-thick serial sections of the musculocutaneous nerve in 5 fresh cadaver arms. The group of fasciculi of the nerve to the biceps, the nerve to the brachialis, and the lateral cutaneous nerve of the forearm were constantly located from lateral to medial. The fasciculi of the nerve to the biceps traveled proximally in the musculocutaneous nerve for an average distance of 63 mm.

Acromion↗
PubMed1993

Serum level of magnesium attained in magnesium sulfate therapy for severe preeclampsia.

Serum magnesium levels were determined in severe preeclampsia patients who were treated with magnesium sulfate infusion. Forty-four patients with diagnosis of severe preeclampsia between 30-41 weeks gestation, given a conventional 5 g magnesium sulfate intravenous bolus infusion and 1 g/hr continuous infusion and continued 24 hours postpartum, were prospectively studied. The mean pre-treatment magnesium level was 2.3 +/- 0.3 mg/dl which was similar to those reports. The levels measured at 1/2, 1, 2, 4, 12 and 24 hours after initiation of magnesium infusion were 4.8 +/- 0.4, 4.7 +/- 0.4, 4.5 +/- 0.3, 4.7 +/- 0.3, 5.4 +/- 0.3 and 5.9 +/- 0.3 mg/dl respectively. After delivery the levels were measured immediately, 12, and 24 hours and found to be 4.7 +/- 0.4, 4.9 +/- 0.4, and 5.2 +/- 0.3 mg/dl respectively. The serum magnesium levels were also varied with maternal weight, the dose regimen used in our study is appropriate for Asian pregnant women whose body weight usually less than 70 kg.

Adult↗
PubMed1992

Urinary vanilmandelic acid determination using column chromatography.

A simple method for determination of urinary vanilmandelic acid, VMA, using column chromatography was described. The interfering substances in the urine were eliminated by passing the urine through a Dowex-1 x 2, 50-100 mesh, column and washing the column with distilled water. The VMA was eluted from the resin using K2CO3-containing 3.0 mol/l NaCl solution and determined spectrophotometrically after periodate oxidation reaction. This method yielded a standard curve which was linear up to 50 mg/l. The recoveries of the method as determined by addition technique ranged from 92.1 to 100.4 per cent with an average of 97.3 per cent. The method yielded satisfactory precisions with the coefficients of variation (C.V.) of less than 5.50 and 6.95 per cent for within-run and between-run experiments respectively. The method was sensitive to the concentrations of 1.2 mg/l with recovery of 92.5 per cent and 2.6 mg/l with the recovery of 109.9 per cent for the standard and urinary VMA respectively. No interfering effects were found from epinephrine and norepinephrine (up to 2,000 micrograms/l), acetylsalicylic acid (up to 50 mg/l) and homovanillic acid (up to 10 mg/l) added to the pooled urine. The correlation coefficients (r) of 0.909 (n = 100) and 0.905 (n = 100) were obtained when the urinary VMA values determined by the proposed and Pisano's methods and expressed as mg/l and mg/g creatinine were compared respectively. The column could be reused at least 5 times. The technique is suitable for any routine clinical laboratory.

Chromatography↗
PubMed1991

Urinary neopterin in patients with systemic lupus erythematosus.

Concentrations of neopterin were measured in urine specimens from 35 patients with active and eight with inactive systemic lupus erythematosus (SLE). Compared with those of apparently healthy controls, neopterin concentrations were higher in patients with active disease (P less than 0.001) and with inactive disease (P less than 0.01), those in patients with active disease being significantly higher than those in patients with inactive disease (P less than 0.001). The correlation between the neopterin concentration and evidence of disease activity was good. All of the patients with clinically active SLE had increased neopterin, but for only 37.5% (three of eight) did the neopterin concentration exceed the upper normal limit during clinical remission. The increase in neopterin concentration did not correlate with clinical courses or severity of renal function. Moreover, serial determinations of neopterin in active SLE patients showed a rapid decrease of initially high concentration, paralleling a decline of clinical activity after initiation of medical therapy. Thus, urinary neopterin may be a useful marker for monitoring disease activity in SLE patients.

Adolescent↗
PubMed1990

Prospective study on premature labor with magnesium sulfate.

The efficacy of magnesium sulfate was evaluated as the primary tocolytic agent in the management of patients at risk for premature delivery. Fifteen patients presenting with premature labor between 28 and 35 weeks gestation, given titrated dose intravenously magnesium sulfate till uterine contraction ceased, were prospectively studied. All patients received an additional oral beta-mimetic agent once labor was arrested till 36 weeks gestation. All cases had intact membranes and no contraindications for tocolytic therapy. Tocolysis for more than 72 hours was achieved in 14 cases (93.3%). Delayed tocolysis of more than 7 days was achieved in 13 cases (86.7%). The mean dosage to achieve tocolytic effect was 3.17 g/hr (SD 0.08). The mean serum magnesium level to achieve such effect was 5.38 mg/dl (SD 0.88). Magnesium sulfate was found to be easy to administer and clinically efficacious with minimal adverse effects. It may be used as the first line of tocolytic therapy where beta-sympathomimetic drugs are contraindicated.

Adult↗
PubMed1984

Non-transferrin plasma iron in beta-thalassaemia/Hb E and haemoglobin H diseases.

Non-transferrin plasma iron concentrations were determined in 45 normal controls and in 37 patients with Hb H disease and 104 patients with beta-thalassaemia/Hb E disease. This revealed that non-transferrin plasma iron exists in cases with severe iron overload, more striking in beta-thalassaemia/Hb E than in Hb H disease. Non-transferrin plasma iron is associated with higher transferrin iron saturation and higher plasma ferritin levels. The most striking finding was the significantly higher non-transferrin plasma iron in splenectomized patients with beta-thalassaemia/Hb E disease than in the non-splenectomized patients. In view of the potential toxicity of non-transferrin iron, this fraction of iron may be responsible for tissue damage in these patients especially after splenectomy.

Hemoglobin E↗
PubMedDate not supplied

Human pancreatic alpha-amylase. II. Effects of pH, substrate and ions on the activity of the enzyme.

Purified human pancreatic alpha-amylase (alpha-1,4-glucan 4-glucano-hydrolase, EC 3.2.1.1) was found to be stable over a wide range of pH values (5.0 to 10.5) with an optimal pH for the enzymatic activity of 7.0. The Michaelis constant of the enzyme at optimal pH and assay conditions was found to be 2.51 mg per ml for soluble starch. Halide ions were required for the activity of the enzyme whereas sulfate and nitrate were not. The order of effectiveness of activation was found to be: Cl- greater than Br- greater than I- greater than F-. Calcium and magnesium were activators at concentrations of 0.001M and 0.005M, respectively, but exhibited inhibitory effects at concentrations higher than 0.005M. At 0.01M ethylenediamine tetraacetic acid (EDTA) concentration the enzymatic activity upon seven min incubation, was inhibited up to 96%. The inhibition of EDTA and calcium could be reversed upon addition of calcium and EDTA, respectively.

Amylases↗
PubMedDate not supplied

Human pancreatic alpha-amylase. I. Purification and characterization.

alpha-Amylase was extracted from human pancreas and purified by using ammonium sulfate fractionation, Sephadex G-100 and DEAE-Sephadex A-50 column chromatography. The enzyme was shown to be homogenous by three different criteria: polyacrylamide disc gel electrophoresis, SDS polyacrylamide gel electrophoresis and analytical ultracentrifugation. The values of SO20,w, D20,w, v, and frictional ration of the enzyme were calculated to be 5.01S, 7.56D, 0.718 ml g-1 and 1.10, respectively. The molecular weight of the alpha-amylase was determined by three different methods: sedimentation velocity-diffusion, conventional sedimentation equilibrium and SDS polyacrylamide gel electrophoresis and was found to be 57,850; 50,100 and 53,200 g mole-1, respectively (average value 53,700). The amino acid composition of the enzyme was determined and compared with those of alpha-amylases from various other sources.

Amino Acids↗