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Biomedical subjects

P Teesdale

Publications and source records attributed to P Teesdale.

At least 19 recordsLinked to original sources
PubMed1997

Comparison of in vivo red cell survival of donations collected by Haemonetics MCS versus conventional collection.

The Haemonetics Multicomponents System (MCS) cell separator allows concurrent donation of red cells in addition to platelets and/or plasma, thus increasing the versatility of apheresis donations. In vivo survival of autologous red cells obtained by MCS was compared with red cells collected conventionally. In this cross-over controlled study, five male volunteers donated one unit of red cells by MCS and one unit of whole blood by the conventional manual method, 3 months apart. After storing donations in SAG-M for 35 days under standard conditions, radioactive (51Cr)-labelled autologous red cells were injected into each donor. The post-transfusion recovery (PTR) of red cells at 24 and 48 h did not show any significant difference between red cells obtained manually and by MCS, indicating that processing differences have no detrimental effects on red cell survival.

Blood Donors↗
PubMed1991

Development of non-Rh antibodies in volunteers stimulated for the production of hyperimmune anti-D.

At the North London Blood Transfusion Centre, red cells from accredited Rh-D-positive donors, matched for all antigens capable of inducing clinically significant antibodies, are used to stimulate immune plasma donors to achieve higher anti-D levels. Despite such careful matching, antibody to the relatively non-immunogenic M antigen developed in 3 out of 20 NN donors (15%) stimulated with M-positive cells. In general, good responders to the Rh antigen D are good responders to other red cell antigens; our report exemplifies the importance of using fully matched accredited red cells for immune stimulation and the need to perform thorough antibody screening after each stimulation.

Antibody Formation↗
PubMed1990

Clearance of Rh D-positive red cells with monoclonal anti-D.

Two human monoclonal antibodies, one IgG3 and one IgG1, with anti-Rh D specificity, were tested for their ability to clear red cells. Samples of red cells from 12 D-positive subjects were sensitised in vitro with various amounts of antibody, the number of antibody molecules bound to the cells was estimated, and the cells were reinjected into the donor's circulation. Both antibodies mediated clearance but substantially fewer IgG3 than IgG1 antibody molecules were required to produce a given rate of clearance. The IgG3 antibody was slightly more effective than polyclonal anti-D, as judged by comparison with previously published results. Since there is believed to be an association between the rate of red cell clearance by anti-D and the ability of the antibody to suppress D immunisation, the IgG3 monoclonal antibody may prove suitable for the immunoprophylaxis of Rh D haemolytic disease.

Antibodies, Monoclonal↗
PubMed1987

The effect of naturally occurring Rh antibodies on the survival of serologically incompatible red cells.

Investigations on six males with naturally occurring Rh antibodies are described. In two subjects in whom the antibody (one anti-E and one anti-D) could be detected only by a two-stage papain technique, the survival of incompatible red cells was normal. In the remaining four subjects, the antibodies (two anti-E and two anti-D) could be detected by the indirect antiglobulin test and, in these, incompatible red cells were destroyed at an accelerated rate; in two of the subjects, 75-99% of the cells were cleared within 24 h; in the other two, 50% of the cells were cleared within 24 h and the remaining cells were cleared far more slowly. All six antibodies were mainly or wholly IgG; a clear-cut immune response was observed in only one case.

Coombs Test↗
PubMed1987

Swa: a subdivision.

For some time, anomalous serological reactions have been observed when the same anti-Swa sera are tested against red cells from different individuals reported as Sw(a+). A comparative collaborative study using the same collection of Sw(a+) cells and anti-Swa sera was undertaken by 4 reference laboratories, and it was found that Swa represents a heterogeneous group of antigens that can be subdivided into two categories. Both categories, Sw(a+) 700:41 and Sw(a+) 700:-41, were shown to be inherited.

Blood Group Antigens↗
PubMed1984

Structures of Miltenberger class I and II specific major human erythrocyte membrane sialoglycoproteins.

The N-terminal structures of the Miltenberger (Mi-) blood group class I and II specific human MN erythrocyte membrane sialoglycoproteins were determined by manual sequencing of tryptic glycopeptides and various secondary fragments. The Mi-I and Mi-II active glycoproteins were found to exhibit a threonine leads to methionine and threonine leads to lysine exchange, respectively, at position 28 which prevents N-glycosylation of asparagine 26. Due to the absence of the N-glycosidic oligosaccharide chain, the monomeric form of the Mi-I and Mi-II specific glycoproteins possesses a slightly increased sodium dodecyl sulfate/polyacrylamide gel electrophoretic mobility, in comparison to its normal counterpart. Serological studies suggest that antibodies, specific for Mi-I or Mi-II red cells, react with the structurally altered region of the MN glycoprotein.

Amino Acids↗
PubMed1984

Serology and genetics of an MNSs-associated antigen Dantu.

Dantu, a previously undescribed low-incidence red cell antigen, is inherited as a Mendelian dominant character. The Dantu antigen is associated with very weak s antigen, protease resistant N antigen and either very weak or no U antigen. Two of the propositi had previously been shown to have an unusual hybrid MNSs sialoglycoprotein, and it is probably this which carries these unusual N, s and U antigens as well as the Dantu antigen. A study of the family of one propositus suggests, by conventional genetics, that Dantu is not controlled by the MNSs locus; a possible explanation is given. Several examples of anti-Dantu are known, one was found to cause a positive direct antiglobulin reaction on neonatal red cells.

ABO Blood-Group System↗
PubMed1982

Red cell antigen, serum protein and red cell enzyme polymorphisms in Eastern Highlanders of New Guinea.

A series of 1,187 blood samples from eight population groups in the Eastern Highlands of Papua New Guinea were tested for genetic variation in blood groups, serum proteins and red cell enzyme systems. The populations belonged to the language groups Gahuku-Asarc-Bena Bena, Kamano, Yagaria, Keiagana, Fore, Agarabe, Auyana and Tairora. Polymorphic variation was found in the ABO, MNS, P1, Rh, Hp, Tf, SEP, 6-PGD, ADA, MDH, and PGM genetic systems. East to West variation was shown in the language groups; the O, S, R2, and R0 genes increase in frequency from East to West and the A, R1, and M genes decrease in the same direction. In the East higher frequencies were found for the Du antigen, for the PGM21 gene and for a PGM second locus variant. The MDH 3 variant was found in all the populations, its highest value being in the Tairora.

Acid Phosphatase↗
PubMed1982

Red cell antigen, serum protein and red cell enzyme polymorphisms in Karkar Islanders and inhabitants of the adjacent North Coast of New Guinea.

Blood samples from the Waskia and Takia populations of Karkar Island, Papua New Guinea, and other nearby mainland populations, were tested for genetic variation in blood group, serum protein and red cell enzyme systems. Polymorphic variation was present in the ABO, P, MNS, Rh, Lewis, Duffy, Kidd and Gerbich blood group systems, in the Hp and Tf serum protein systems, and in the acid phosphatase, 6-PGD, ADA, PGM, MDH, and G-6-PD enzyme systems. A small number of variants was found in other systems: there were 4 Lu(a+), 1 Kp(a+), 2 C variants in the acid phosphatase system, 6 LDH variants, 1 ADA3-1 and 1 AK2-1 sample. All samples were negative for the red cell antigens Cw, Vw, He, K, Jsa, Dia, Wra, Rd and Marriott, and no variation was observed in the PHI enzyme system. The results are discussed in relation to those obtained on other Papua New Guinea populations.

Acid Phosphatase↗
PubMed1977

Blood groups of the Irish.

Some 1800 blood donors from the Republic of Ireland and Northern Ireland have been tested for the antigens A A1 B H; M N Ss Henshaw; C c Cw D Du E e Ce; Wra; P1; Lua; K k Kpa; Fya Fyb; and Rd (Radin). The results are compared with those already available for Ireland and an attempt is made to interpret them in terms of populations movements. With the exception of the high O frequency in the West, believed to represent the remnants of Mesolithic peoples, the blood groups are very similar to those in the rest of Europe, and show no significant differences between the different provinces in Ireland.

Blood Group Antigens↗
PubMed1976

The blood groups, serum groups, red-cell isoenzymes and haemoglobins of the Sandawe and Nyaturu of Tanzania.

Blood specimens from members of the click-speaking Sandawe tribe of Tanzania and of the adjacent Bantu-speaking Nyaturu tribe have been tested for antigens of 11 blood group systems, for variants of 3 plasma-protein systems and 9 red-cell-enzyme systems, for haemoglobin variants. The results are tabulated and gene frequencies computed. For most systems, the frequencies in the two tribes are similar to one another and, in so far as data are available, similar to the neighbouring Bantu-speaking tribes. The principal genetic difference between the Sandawe and the Nyaturu is in their frequencies of haemoglobin S and of glucose-6-phosphate dehydrogenase deficiency, both of which characters are several times higher in the Nyaturu than in the Sandawe; both characters are protective against falciparum malaria, and this suggests that the Nyaturu have in the past been much more strongly exposed to this infection than the Sandawe.

Black People↗
PubMed1976

An Alberta Cree Indian with a rare Duffy antibody, anti-Fy 3.

Anti-Fy3, found in the serum of an Fy(a-b-) Cree Indian woman believed to have been transfused 5 years previously, caused moderate hemolytic disease of the newborn in her eighth live-born baby. Only three examples of anti-Fy3 are know. It is striking that two were made by non-negro people, amongst whom Fy(a-b-) is extremely rare, and only one by a Negro, in whose people Fy(a-b-) is by far the commonest phenotype. This suggests that Fy(a-b-) may be a heterogeneous phenotype, with the negro version conferring some impediment to immunization by Fya, Fyb or Fy3 antigens. The rare allele Fyx is also present in the kindred of the propositus, and is shown to correspond to weak Fy3 as well as weak Fyb anitgen.

Alberta↗