Biomedical subjects

P T Hung

Publications and source records attributed to P T Hung.

At least 19 recordsLinked to original sources

PubMed1999

Safety and ocular hypotensive efficacy of a single dose of metoclopramide or droperidol in healthy subjects.

The purpose of this study was to evaluate the IOP lowering effect of two topical dopamine antagonists, metoclopramide or droperidol, in healthy subjects. Forty healthy volunteers were randomly assigned to receive, in double-masked fashion, topical administration of a single drop of 0.5% metoclopramide or 0.25% droperidol, with the fellow eye receiving isotonic saline as placebo. IOP was measured before and 1, 3, 5, and 7 hours after instillation of drugs. Ocular irritation and conjunctival congestion were also recorded at the time of each measurement. In the metoclopramide group, the maximal mean percentage change in IOP was -14.4% in tested eyes as compared with -10.8% in placebo-treated eyes 3 hours after instillation. All the changes were not significantly different between the metoclopramide-treated and the placebo-treated eyes at all time points. In the droperidol group, the maximal mean percentage change in IOP was -19.6% in tested eyes as compared with -17.7% in placebo-treated eyes at 3 hours after instillation. There was also no significant difference between the droperidol-treated and the placebo-treated eyes. None of the volunteers reported ocular irritation or conjunctival congestion after instillation of the drugs. In conclusion, while topical droperidol or metoclopramide tended to lower IOP in healthy subjects, the decrease in IOP did not differ significantly from that in placebo-treated eyes. Both drugs appear to be safe. Further studies with larger numbers of subjects at higher doses in glaucomatous subjects are needed before definite conclusions on drug efficacy can be drawn.

Adult↗

PubMed1999

Effects of different concentrations of atropine on controlling myopia in myopic children.

Although 1% atropine effectively slows myopia progression, it is associated with adverse effects, including photophobia, blurred near vision, and poor compliance. We investigated whether lower doses of atropine would control myopia progression. One hundred and eighty-six children, from 6 to 13 years of age, were treated each night with different concentrations of atropine eye drops or a control treatment for up to 2 years. The mean myopic progression in each of the groups was 0.04 +/-0.63 diopter per year (D/Y) in the 0.5% atropine group, 0.45+/-0.55 D/Y in the 0.25% atropine group, and 0.47+/-0.91 D/Y in the 0.1% atropine group. All atropine groups showed significantly less myopic progression than the control group (1.06+/-0.61 D/Y) (p<0.01). Our study also showed that 61% of students in the 0.5% atropine group, 49% in the 0.25% atropine group and 42% in the 0.1% atropine group had no myopic progression. However, 4% of children in the 0.5% atropine group, 17% in the 0.25% atropine group, and 33% in the 0.1% atropine group still had fast myopic progression (>-1.0 D/Y). In contrast, only 8% of the control group showed no myopic progression and 44% had fast myopic progression. These results suggest that all three concentrations of atropine had significant effects on controlling myopia; however, treatment with 0.5% atropine was the most effective.

Adolescent↗

PubMed1999

Epidemiologic study of ocular refraction among schoolchildren in Taiwan in 1995.

PURPOSE: In order to understand and update the prevalence of myopia in Taiwan, a nationwide survey was performed in 1995. METHODS: We stratified the cluster sampling by developmental grading of the city, using a size proportional to the population. Two cities were randomly selected from each city grading. The total number of students enrolled was 11,178, including 5,676 boys and 5,502 girls. The refractive status and corneal radius of each student were measured with an autorefractometer under cycloplegia and checked with retinoscopy. Axial length was measured with biometric ultrasound. RESULTS: The myopic rate was from 12% at the age of 6, it increased to 56% at the age of 12, and then to 76% at the age of 15. A myopic rate of 84% was found for the age range of 16 to 18. The prevalence of high myopia (over -6.0 D) at the age of 18 was 20% in girls and 12% in boys. The mean refractive status became myopic at the age of 9, then increased to -3.92 D in girls and -2.71 D in boys at the age of 18. The increase of axial length is correspondent with the progression of myopia. The anterior chamber depth (ACD) was deeper with age and the severity of myopia, whereas the corneal curvature remained unchanged. The lens thickness became thinner from age 7 to 13, then it became thicker with age and the severity of myopia after age 15. The prevalence and degree of myopia in girls was more severe than in boys. CONCLUSIONS: The prevalence of myopia in Taiwan increased year by year. The increase in severity and prevalence of high myopia may be due to earlier onset.

Adolescent↗

PubMed1998

The cycloplegic effects of cyclopentolate and tropicamide on myopic children.

Thirty-seven myopic children were given either 1-2 drops of 1% cyclopentolate or 1% tropicamide twice with 5 min intervals to evaluate the time course and maximal cycloplegic effect of both agents. The other fifteen subjects were given 1% tropicamide initially, then 1% cyclopentolate given after 30 min of maximal effect of tropicamide appeared to evaluate whether the effect of cyclopentolate was superior to tropicamide. Cycloplegic refraction was measured with an auto-refractometer (Topcon RK-3000) before drug delivery and every 15 min thereafter, for 90 min. The maximal cycloplegic effect of cyclopentolate was around 45 min, then it remained stable until 90 min after the last instillation. The effect of tropicamide was faster than that of cyclopentolate. It was around 30 min, then it stabilized until 75 min. The extra effect of cyclopentolate over tropicamide was minimal (only -0.1D). The power of cornea and astigmatism were not affected by either agent. However, a big variation in astigmatism was noted during the course, especially with cyclopentolate. This study suggests that 1% tropicamide should be a good agent for routine refractive status checking on myopic children.

Accommodation, Ocular↗

PubMed1998

The effect of intravitreal injection of atropine on the proliferation of scleral chondrocyte in vivo.

Atropine was found to be effective in arresting the progression of myopia. However, the actual mechanism is still unclear. Thus, we tried to investigate the in vivo effect of atropine on the proliferation of scleral chondrocytes in chicks of form-deprivation myopia. Twenty chicks were equally divided into 4 groups which included intravitreal injection of normal saline (IVN), IVN with goggling (IVNG), intravitreal injection of atropine (1%) (IVA), and IVA with goggling (IVAG) groups. Intravenous injection of bromodeoxyuridine (BrdU) (30 mg/kg) from subaxillary vein was performed 2 hours before being sacrificed. The eyeballs were then fixed in 10% neutral-buffered formalin at 4 degrees C. Standard BrdU immunohistochemical staining was performed. The BrdU labeling index was obtained from the average of positive labelings of BrdU in scleral chondrocytes for every 100 counting cells in posterior poles and anterior scleral margins by two experienced technicians. The BrdU index on the anterior scleral margin of the IVAG group was less than that of the IVNG group. The index on the anterior scleral margin of the IVNG group was higher than the IVN group. Although the index on the posterior poles of the IVNG group was also higher than the IVN group, it was statistically not different. Also, no statistical difference was found between IVN and IVA on the anterior scleral margins or posterior poles. The index was significantly different on the anterior scleral margins, but not on the posterior pole among each group. Therefore, intravitreal injection of atropine could inhibit the proliferation of chondrocytes on the anterior margins of sclera, but not the posterior poles in form-deprivation myopia.

Animals↗

PubMed1998

Influence of destruction of retina-RPE complex on the proliferation of scleral chondrocytes in chicks.

We studied the role of the retina-retinal pigment epithelium (RPE) complex in the proliferation of scleral chondrocytes in chicks. Seventy-two chicks were allocated to one of four groups: intravitreal gentamicin (400 microg) injection (destruction of retina-RPE complex); intravitreal gentamicin injection with goggling; goggling only (form-deprivation myopia); and intravitreal saline injection (control). The chicks were killed and retina-RPE complexes were harvested under a microscope. Retina-RPE complexes were then co-cultured with primary culture of first day scleral chondrocytes in Transwell-COL co-culture systems (Costar), with two different pore sizes (0.4 and 3.0 microm) and serum-deprivation medium. An MTT assay was performed at A550 after 4 days. In the 0.4 microm pore size system, the absorbency at A550 showed no differences between groups. However, in the 3.0 microm pore size system, the absorbency at A550s in the intravitreal gentamicin groups was significantly lower than in the control and the goggle groups (p<0.05), indicating that destruction of the retina-RPE complex inhibited chondrocyte proliferation. The absorbency in the goggle group was higher than in the control group (p<0.05). These results indicate that the retina-RPE complex exerts a positive effect on the proliferation of scleral chondrocytes via a molecule sized between 0.4.and 3.0 microm in diameter.

Animals↗

PubMed1998

Effects of lid suturing and trans-scleral cryotherapy on ocular growth in a piglet model.

PURPOSE: To study whether lid suturing can induce axial myopia and explore whether trans-scleral cryotherapy can affect ocular growth in piglets or not. METHODS: A total of fourteen 2- to 3-week-old piglets were studied. Three groups were included: lid suture, cryotherapy, and both lid suture/cryotherapy groups. The lid suturing group (N = 6) was given lid suturing to produce visual deprivation. The cryotherapy group (N = 4) received trans-scleral cryotherapy 360 degrees to encircle anterior to the equator. Both treatment groups (N = 4) received both cryotherapy and lid suturing. The cycloplegic refraction, corneal power, biometric axial length, and intraocular pressure (IOP) were measured before the experiments and 4 months later. RESULTS: Mild axial myopia was induced in five lid-sutured eyes of the lid suturing group. The ocular refraction and eye size of cryotherapy eyes were not different from the control eyes in the cryotherapy group. More myopia was found in all four piglets that received both cryotherapy and lid suturing. CONCLUSIONS: Lid suturing can induce axial myopia; however, cryotherapy did not affect normal eye growth and did not prevent the development of lid suturing myopia in piglets.

Animals↗

PubMed1997

Biometry and primary angle-closure glaucoma among Chinese, white, and black populations.

PURPOSE: Primary angle-closure glaucoma (PACG) is more prevalent among Chinese than whites. The authors tested the hypothesis that Chinese have shallower anterior chambers than do whites, a factor that may be related to PACG prevalence. METHODS: The authors compared anterior chamber depth, axial length, radius of corneal curvature, and refractive error among 531 Chinese, 170 whites, and 188 blacks older than 40 years of age using the same model of instruments and identical technique. RESULTS: Mean anterior chamber depth and axial length did not differ significantly for the three groups. Whites had a significantly higher prevalence of hyperopia > 2 diopters than did Chinese. Radius of corneal curvature was significantly smaller among Chinese than whites or blacks. CONCLUSIONS: These results suggest that Chinese do not differ on a population basis from other ethnic groups in many of the biometric risk factors known to be of importance for PACG. It will be necessary to identify other ocular biometric parameters to explain the excess burden of PACG among Chinese, which may improve the effectiveness of screening for this disease in all populations.

Adult↗

PubMed1997

Intraocular lens position and anterior chamber angle changes after cataract extraction in eyes with primary angle-closure glaucoma.

PURPOSE: To prospectively study the anterior chamber angle in eyes with chronic primary angle-closure glaucoma (PACG) having posterior chamber intraocular lens (IOL) implantation and evaluate IOL position and intraocular pressure (IOP). SETTING: National Taiwan University Hospital, Taipei, Taiwan. METHODS: Using Scheimpflug image processing, the anterior chamber angles were studied in 20 consecutive eyes with chronic PACG and 10 control eyes before and after posterior chamber IOL implantation. Anterior chamber depth, chamber angle width, IOL position, and IOP change were evaluated. RESULTS: Mean anterior chamber depth in the PACG group was 2.04 +/- 0.29 mm preoperatively and 3.44 +/- 0.16 mm postoperatively. The anterior chamber angle widened significantly in relation to the superior, inferior, temporal, and nasal quadrants after surgery. The degree of IOL tilting and decentration was the same in both the PACG and control groups. All eyes in the PACG group maintained an IOP under 21 mm Hg during the 6 month follow-up. Eighty-four percent maintained or decreased their antiglaucoma medication; 16% required increased medication. CONCLUSION: Cataract extraction with posterior chamber IOL implantation in eyes with PACG controlled IOP well in most cases.

Aged↗

PubMed1997

The antimicrobial susceptibility of Mycobacterium chelonae isolated from corneal ulcer.

PURPOSE: To determine the in vitro susceptibility of Mycobacterium chelonae isolates from corneal ulcers to various traditional and newly-developed antimicrobial agents, alone or in combination. METHODS: Fifteen strains of M. chelonae isolated from corneal ulcers were collected at the National Taiwan University Hospital from 1989 to 1993. Susceptibility to antimicrobial agents was tested by the broth microdilution method to determine the minimum inhibitory concentration (MIC). The antimicrobial effects of combinations of antimicrobial agents were assessed by the checkerboard titration method to determine the fractional inhibitory concentration (FIC) index. RESULTS: The MIC results showed that traditional antituberculous drugs had poor activity against M. chelonae. In the aminoglycoside group, tobramycin and amikacin had better activity than gentamicin. Among macrolides, clarithromycin was especially effective, with an MIC ranging from 0.125 to 1 microgram/ml. Among various beta-lactam antibiotics, imipenem was the only one to demonstrate good anti-mycobacterial activity. Of the quinolone group, ciprofloxacin was the most effective, with an MIC ranging from 0.5 to 16 micrograms/ml. Combination of an aminoglycoside with imipenem, ciprofloxacin or clarithromycin all showed antagonistic effect. CONCLUSIONS: The results suggested that amikacin, clarithromyicn, imipenem and ciprofloxacin had good in vitro antimicrobial activity against M. chelonae. However, no synergistic effect could be demonstrated for combinations of an aminoglycoside with other effective drugs.

Anti-Bacterial Agents↗

PubMed1997

Inhibition of RPE cell-mediated matrix adhesion and collagen gel contraction by crovidisin, a collagen-binding snake venom protein.

PURPOSE: Cell-mediated collagen gel contraction plays an important role in the pathogenesis of proliferative vitreoretinopathy (PVR). Anti-adhesion therapy has been suggested as a promising strategy in the treatment of PVR. Crovidisin, a snake venom protein isolated from Crotalus viridis, has been shown to bind selectively to collagen and to inhibit collagen-induced platelet aggregation. In the present study, the effectiveness of crovidisin in inhibiting the attachment of retinal pigment epithelial (RPE) cells to collagen, and RPE cell-mediated collagen gel contraction, was evaluated. METHODS: Fluorescein isothiocyanate (FITC)-conjugated crovidisin was prepared and used to evaluate its binding affinity for collagen type I, fibronectin, vitronectin, and laminin. The inhibitory effect of crovidisin on RPE cell-mediated extracellular matrix attachment and collagen gel contraction was evaluated by cell adhesion and type I collagen gel contraction assays. The cytotoxic effect of crovidisin was examined with a cell proliferation assay, using the Alamar blue method. Flavoridin, an Arg-Gly-Asp-containing peptide from viper venom, was used for comparison. RESULTS: FITC-conjugated crovidisin bound selectively to collagen type I with high affinity. It did not bind to other matrix proteins, including fibronectin, vitronectin and laminin, nor to RPE cells. Crovidisin inhibited RPE cell attachment to type I collagen in a dose-dependent manner. This inhibitory effect was enhanced by the presence of flavoridin. Crovidisin also dose-dependently inhibited RPE cell-mediated type I collagen gel contraction. Crovidisin was non-toxic to RPE cells. CONCLUSIONS: Crovidisin, a snake venom-derived collagen-binding protein, possessing an inhibitory activity on RPE cell-collagen interaction and RPE cell-mediated collagen gel contraction, may be a useful tool for studying cell-collagen interaction, and a potential anti-adhesion therapeutic agent for ocular disorders in which cell-collagen interaction in involved, such as PVR.

Animals↗

PubMed1997

The choroidal blood flow response after flicker stimulation in chicks.

Form-deprivation myopia (FDM) can be prevented by exposing the animal to stroboscopic illumination (10 Hz). Flicker illumination is known to stimulate the release of dopamine (DA) from the retina. We hypothesize that DA was released and diffused into the choroid. To prove this hypothesis, we decided to undertake an investigation in chicks and measure choroidal blood flow (ChBF) during stimulation of the ocular fundus with diffuse flicker. White Leghorn chicks (2 weeks old) were used for this study. Different flash stimulations (5 Hz approximately 50 Hz) were given for 3 minutes, then ChBF was recorded with the PeriFlux flowmeter simultaneously and continuously for 5 minutes. Some birds are recorded up to 120 minutes to find out any late-onset effect. The ChBF was increased after flicker stimulation. The difference was statistically significant in 10 Hz, 20 Hz, and 30 Hz. The ChBF can maintain 20% higher for 60 minutes. Therefore, flicker illumination preventing FDM may be induced by the hyperactivity of ganglion cells, then stimulates the release of DA from the retina and suppresses the development of myopia.

Animals↗

PubMed1997

Anterior chamber angles shallowing and intraocular pressure after topical pilocarpine.

Pilocarpine has been well recognized as the drug of choice for acute angle-closure glaucoma. The purpose of this study is to clarify quantitatively the change of anterior chamber angle and depth with the passage of time after pilocarpine drop administration. Chamber angles of the four quadrants and chamber depths were measured in 18 normal subjects by Scheimpflug Video Image prior to and 30, 60, and 180 minutes after administration of one drop of 4% pilocarpine in one eye, while the opposite eye served as control. The anterior chamber angle and depth showed a significant shallowing at 30, 60 and 180 minutes after 4% pilocarpine administration with a maximal effect of -3.61 degrees and -0.15 mm at 30 minutes, respectively, while the reduction of intraocular pressure reached its maximal effect at 180 minutes. These facts should be well understood in the treatment of angle-closure glaucoma.

Adult↗

PubMed1997

The effect of 0.5% timolol maleate on the ocular perfusion of ocular hypertensive patients by scanning laser flowmetry.

To understand the effect of 0.5% timolol maleate on the ocular perfusion of the optic disc and macula in ocular hypertensive patients, we enrolled 10 males and 15 females without any systemic or ocular disease, except intraocular pressure higher than 20 mmHg. Their average age was 33 +/- 13 y/o (range 14-45). Under the randomized, double-masked design, one drop of 0.5% timolol maleate was given in one eye, and placebo in the fellow eye. Heart rate, blood pressure, intraocular pressure, and ocular perfusion were measured at baseline, and then 30 minutes and 2 hours after treatment. Ocular perfusion was measured by Heidelberg Retina Flowmeter (Heidelberg Engineering GmbH, Heidelberg, Germany). We used 10 degrees measurement field and 10 x 10 pixels measurement frame. Four areas were measured, i.e., temporal upper, temporal lower, and nasal parts of the optic disc, and macula. In comparison to the baseline, the treated eyes had a slight reduction of blood flow, volume, and velocity 30 minutes after treatment, but these parameters came back close to the baseline value at 2 hours after treatment. Similar changes were also noted in the control eyes. The results showed that a single drop of 0.5% timolol had minimal effects on the retinal and macular circulation within 2 hours after treatment.

Adolescent↗

PubMed1997

The regulation of the scleral growth associated with deprivation myopia in chicks.

The mechanism of axial elongation caused by experimental or clinical myopia is still unknown. We sought to explore the changes of scleral chondrocytes during myopia formation through the cell biology model. White Leghorn chicks were used for this study. The right eye was covered with a translucent goggle after hatching, and the left eye was left uncovered for control. The chicks were maintained on 12 hours light-dark cycle for two weeks, then sacrificed every other day and the eyeballs removed for study. Our results in the primary culture of scleral chondrocytes showed that the densities of chondrocytes on myopic eyes were significantly higher than those of the controlled non-myopic eyes, and 3H-thymidine incorporation rate also increased with the increasing of the concentration of fetal bovine serum. The PCNA index of chondrocytes in myopic eyes was also higher than that of the controlled non-myopic eyes. Thus, axial elongation of experimental myopia in the chick is the result of active tissue remodeling rather than passive scleral stretching alone.

Animals↗

PubMed1997

Automated perimetry in primary open-angle glaucoma.

Primary open-angle glaucoma (POAG) is one of the leading causes of blindness worldwide. Patients are usually asymptomatic until severe anatomic and functional damage occurs. Visual field examination is important in managing glaucoma patients, especially in the treatment and follow-up of moderate to advanced cases. This study was designed to investigate the patterns of automated visual field defects in POAG. We studied 296 POAG patients from the Glaucoma Clinic of the National Taiwan University Hospital from January 1986 to June 1996. The perimetry was performed using the Octopus program 32 or 38, or Humphrey program 30-2, and the results were analyzed according to the Glaucoma Hemifield Test design. The depth and frequency of locations of visual field defects were computed, and stratified by age and intraocular pressure (IOP). The results suggested that the most frequently affected locations in POAG were paracentral areas, while the deepest scotoma appeared in the upper and lower nasal areas. Patients with IOP higher than 25 mmHg or older than 50 years had higher mean sensitivity losses in both superior and inferior hemifields. The results of this study revealed the patterns of automated visual field defects in POAG may be a useful guide for the management of POAG patients.

Adolescent↗

PubMed1997

Biometric study of acute primary angle-closure glaucoma.

We investigated the biometric characteristics of acute primary angle-closure glaucoma (PACG) patients and sought guidelines to predict subjects at high risk of acute attack. We enrolled 80 patients who suffered acute attack of angle-closure glaucoma and 60 nonglaucoma subjects. The biometric data, measured with A scan ultrasonography, and the outcomes after various treatments were analyzed. Six patients were excluded because they were lost to follow-up. In the acute PACG patients, the axial length (mean +/- standard deviation) was 22.25 +/- 0.77 mm, the anterior chamber depth (corneal thickness included) was 2.28 +/- 0.23 mm, and the lens thickness was 4.94 +/- 0.28 mm. In the nonglaucoma subjects, the axial length was 23.26 +/- 0.76 mm, the anterior chamber depth (corneal thickness included) was 3.11 +/- 0.25 mm, and the lens thickness was 4.48 +/- 0.30 mm. An anterior chamber depth of less than 2.70 mm was the most sensitive (94%) and specific (94%) parameter to differentiate acute PACG patients from nonglaucoma subjects. The intraocular pressure was controlled within the satisfactory range in 58 patients by laser iridectomy, with or without antiglaucoma medication. Eight patients needed additional gonioplasty and eight required filtering surgery to control the intraocular pressure. Our findings provide useful information about the biometric data of eyes of patients with acute angle-closure glaucoma and offer a useful guide to predict those patients at high risk of acute attack.

Acute Disease↗

PubMed1997

Influence of axial length on visual field defects in primary open-angle glaucoma.

With the high frequency of myopia in Taiwan, potential complications or associated conditions, such as glaucoma, are of great concern. To investigate the role of axial length in glaucoma, we enrolled 307 primary open-angle glaucoma (POAG) patients from 1986 through 1996. For the control group, 124 persons were recruited from a survey of a non-glaucoma population and the Ophthalmology Out-patient Department of the National Taiwan University Hospital. Routine eye examination, stereophotography of the optic disc, automated visual field tests, and A-scan ultrasonography were performed on each patient. The Glaucoma Hemifield test was used for analysis of visual field results. The mean axial length was longer in the POAG group than in the control group, especially in the younger age groups (40-59 yr). The POAG group was divided into a short-axial-length (SAL, axial length < 26 mm) group and a long-axial-length (LAL, axial length > or = 26 mm) group. Both subgroups had the deepest visual field defects in the upper and lower nasal areas. The LAL group had deeper visual field defects and the defects were more frequently involved in all sectors analyzed than the SAL group defects. The upper visual field had deteriorated more in the SAL group, whereas the depth of scotoma was similar in the upper and lower hemifields in the LAL group. Our results support the idea that glaucoma patients have a longer axial length than people without glaucoma, and that visual field defects are more pronounced in patients with LAL than in those with SAL.

Adult↗