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Biomedical subjects

P Speck

Publications and source records attributed to P Speck.

At least 19 recordsLinked to original sources
PubMed2001

The royal free interview for spiritual and religious beliefs: development and validation of a self-report version.

BACKGROUND: Spiritual beliefs are rarely considered in psychological or medical publications. We recently published the psychometric properties of an interview designed to measure religious and spiritual belief. In this study, we aimed to develop this instrument further as a self-report questionnaire and to make it more comprehensive by including measurement of spiritual experiences in addition to faith or intellectual assent. METHODS: Based on extensive discussion with colleagues, advice from users of the interview and comments from respondents, a self-report format was designed. We then evaluated the final format of the questionnaire in terms of (1) patterns of response and demographic predictors of beliefs; (2) test-retest reliability and internal consistency; (3) criterion and internal validity; and (4) the nature of spiritual experiences and their relationship to beliefs and strength of beliefs. RESULTS: Two hundred and ninety-seven people took part in the validity and reliability tests of the questionnaire. Criterion validity, predictive validity, internal consistency and test-retest reliability were acceptably high. The instrument consistently differentiated between people with high and low spiritual beliefs. CONCLUSIONS: This instrument is brief and simple to complete. We would recommend that measures of religious and/or spiritual belief like this be more widely applied in health services research as they evaluate aspects of people's lives that go somewhat further than health status or quality of life.

Adult↗
PubMed2000

Epstein-Barr virus entry utilizing HLA-DP or HLA-DQ as a coreceptor.

Epstein-Barr virus (EBV) glycoprotein gp350/gp220 association with cellular CD21 facilitates virion attachment to B lymphocytes. Membrane fusion requires the additional interaction between virion gp42 and cellular HLA-DR. This binding is thought to catalyze membrane fusion through a further association with the gp85-gp25 (gH-gL) complex. Cell lines expressing CD21 but lacking expression of HLA class II molecules are resistant to infection by a recombinant EBV expressing enhanced green fluorescent protein. Surface expression of HLA-DR, HLA-DP, or HLA-DQ confers susceptibility to EBV infection on resistant cells that express CD21. Therefore, HLA-DP or HLA-DQ can substitute for HLA-DR and serve as a coreceptor in EBV entry.

Cell Line↗
PubMed1999

Epstein-Barr virus (EBV) infection visualized by EGFP expression demonstrates dependence on known mediators of EBV entry.

Studies of Epstein-Barr virus (EBV) infection and pathogenesis are limited by the lack of recombinant EBV bearing a reporter gene. Currently such studies typically utilize cellular transformation and immortalization as indicators of virus infection which takes several weeks. To investigate the dependence on known cellular receptors for EBV infection, a novel enhanced green fluorescent protein (EGFP) expressing virus, designated EBfaV-GFP, was used to infect a variety of different cell types. EBfaV-GFP infects B lymphoma-derived cell lines, lymphoblastoid cell lines (LCLs) and primary B cells, as judged by expression of EGFP. Ability of clonal cells to be infected with EBfaV-GFP correlates with expression of the known EBV entry mediators CD21 and HLA-DR. EGFP-expressing LCLs were derived by infection of primary B cells with EBfaV-GFP. Cells of the Jurkat line, derived from T lymphocytes, could not be infected, and infected primary lymphocytes did not include appreciable numbers of CD2-positive cells, showing that EBV rarely infects T cells. Expression of EGFP by EBV provides the opportunity to rapidly visualize infection in living cells and better delineate populations of infected cells.

Animals↗
PubMed1999

The effect of spiritual beliefs on outcome from illness.

We aimed to assess the role of spiritual belief in clinical outcome of patients nine months after hospital admission. Two hundred and fifty patients admitted to a London teaching hospital were recruited and followed up for nine months. Outcome measures were clinical status as recorded in the outpatient records and patients' self reported health status and beliefs. A hundred and ninety-seven (79%) patients professed some form of spiritual belief, whether or not they engaged in a religious activity. Strength of belief was lower in patients who were in a more serious clinical state on admission (F = 3.099, d.f. = 2 and 192, p = 0.05). Case note information was available nine months later for 234 patients (94%) and contained useful information for judging clinical outcome in 189 (76%). Patients with stronger spiritual beliefs were 2.3 times more likely (CI = 1.1-5.1, p 0.033) to remain the same or deteriorate clinically nine months later. Other predictors of poor outcome were male gender and sleep disturbance at time of admission to hospital. We conclude that a stronger spiritual belief is an independent predictor of poor outcome at nine months in patients admitted to two acute services of a London hospital. It is more predictive of outcome than physical state assessed by clinicians, or self-reported psychological state, at admission.

Adult↗
PubMed1999

Global perspectives.

Explore the source record for details and available documents.

Developing Countries↗
PubMed1998

Precipitous clearance of herpes simplex virus antigens from the peripheral nervous systems of experimentally infected C57BL/10 mice.

Clearance of herpes simplex virus (HSV) from spinal ganglia of experimentally infected mice is known to be dependent on CD8+ T-cells but not on destruction of infected neurons, consistent with a non-cytolytic Tc2 response in the peripheral nervous system. Here, we demonstrate the striking rapidity of such a response in C57BL/10 mice. The number of neurons containing viral DNA and viral antigens increased until 136 h after inoculation of virulent HSV type 1 (strain SC16) into flank skin. Subsequent disappearance of HSV DNA and antigens from infected ganglia was virtually complete only 8 h later. A consistent and unexpected observation was detection of viral antigens in sensory nerve axons for at least 8 h after their disappearance from neuronal somas, raising the intriguing possibility that virus or viral proteins may be transported distally after infection has been terminated.

Animals↗
PubMed1998

Development of an instrument to measure terminal restlessness.

Terminal restlessness is a clinical phenomenon that is frequently observed but poorly defined. Its management is important in providing good quality palliative care. We present the development of an objective observer-rated instrument to measure terminal restlessness.

Humans↗
PubMed1995

The Royal Free interview for religious and spiritual beliefs: development and standardization.

We present the development and standardization of a measure of spiritual, religious and philosophical beliefs. An interview was constructed based on on-going studies by the authors of the nature and strength of belief held by people hospitalized with an acute illness. The interview was tested with three standard populations--staff of a teaching hospital; attenders to an inner city general practice; and people with clearly defined, devout religious beliefs--in order to establish population norms, validity and reliability for each question. The interview performed well with satisfactory validity and high internal and test-retest reliability. It is not presented, however, as a final product which will meet all needs in this complicated area of study. Rather, we have attempted to refine a measure of spiritual and religious belief that might apply to people with a range of personal and public faiths. It is clear that people are able to express these aspects of their lives in a way that can be measured with acceptable reliability and validity. We believe that this interview could, therefore, be applied in any medical, psychological or social setting in which a measure of belief is sought.

Adult↗
PubMed1994

Spiritual and religious beliefs in acute illness--is this a feasible area for study?

In this paper we discuss the measurement of spiritual, religious and philosophical beliefs in patients admitted to hospital with acute physical illness. The discussion is based on the results of a preliminary study of the beliefs of 300 patients studied by face-to-face interview and questionnaire. Principal outcome measures at 6 months were self reported psychological and belief scores and physical state as reported in the medical records. Two thirds of patients reported a religious and/or spiritual belief system. Strength of belief was not associated with social, psychological or diagnostic factors. However, those patients with a religious and/or spiritual life view who expressed strong beliefs were likely to fare less well clinically. We conclude that empirical study of patients' beliefs is possible, though care must be exercised over the measures used. Beliefs were at least as predictive of outcome as other social and psychological factors. This area is of considerable importance to patients and should not be neglected by carers or researchers.

Acute Disease↗