Biomedical subjects

P Soldani

Publications and source records attributed to P Soldani.

57 records · Page 4Linked to original sources

PubMedDate not supplied

Doxorubicin storage in myocardial tissue of reserpine- and nicardipine-pretreated rats.

While reserpine and nicardipine pretreatment induced a more or less marked decrease in typical red-orange doxorubicin fluorescence in certain rat tissues, such as spleen, kidney and large intestine, the myocardial tissue (right atrium and left ventriculum) did not show any significant variations in nuclear doxorubicin storage. This different behaviour of cardiac tissue may be related to the well-known doxorubicin cardiotoxicity.

Animals↗

PubMedDate not supplied

Micro-determination of amino acid composition of proteins electroblotted onto polyvinylidene difluoride membranes.

A method for determination of amino acid composition of proteins separated by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes is described. A single blotted band containing 50 to 200 pmoles of protein was cut out and submitted to acid hydrolysis with HCl followed by derivatization with phenylisothiocyanate. The amino acid derivatives were separated by reverse phase high-performance liquid chromatography. Bovine serum albumin, lysozyme, myoglobin, ovalbumin, soybean trypsin inhibitor and carbonic anhydrase were analyzed; the results revealed a good correspondence with reported values. This can be considered an analytical method to determine the amino acid composition of samples from microquantities of protein mixtures, particularly in those cases in which SDS-polyacrylamide gel electrophoresis is the most suitable separation system.

Amino Acids↗

PubMedDate not supplied

Influence of diazepam on rat cardiac noradrenergic innervation in response to noise stress.

The effects of pretreatment with a benzodiazepine (diazepam) on the cardiovascular system of rats exposed to noise were evaluated. Animals were given diazepam before noise treatment; time exposure was one and six consecutive hours. Examination of preparations (corresponding portions of right atrium and ventricle), obtained using the histofluorescence method, showed that noise treatment increased the density of noradrenergic pattern. In diazepam-pretreated tissues the fluorescent fibres resulted to be thinner and less numerous, in particular after one hour of noise exposure. Our present data demonstrate that diazepam exerts a protective action on the heart, more evident after one hour of treatment.

Adrenergic Fibers↗