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Biomedical subjects

P Simard

Publications and source records attributed to P Simard.

At least 37 records · Page 2Linked to original sources
PubMed1986

Isolation, purification and culture of Sertoli cells from immature piglet testes.

Several studies suggest a role of Sertoli cells in the control of Leydig cell steroidogenesis. In order to verify this hypothesis, we have developed a system for the purification of pig Sertoli cells. These cells were then characterized by their morphological appearance in light and electron microscopy, their ability to bind [125I]follicle stimulating hormone (FSH) and their functional capacity as evaluated by adenosine 3',5' monophosphate (cAMP) accumulation and lactate production when in primary culture under basal and FSH-stimulated conditions. Crude Sertoli cell suspensions from immature porcine testes were fractionated on discontinuous Percoll gradients (densities 1.025, 1.039, 1.055, 1.080 g/ml). Highly purified Sertoli cells were contained in the second band (d: 1.039) generated on the gradient. These cells demonstrated morphological and functional integrity as evidenced by binding specifically [125I] FSH and by responding to FSH stimulation (by an increased production of cAMP and lactate after 3 days in primary culture), but not to human chorionic gonadotrophin (hCG). This preparation represents a useful model for the study of Sertoli cell functions and their interation with Leydig cells in the regulation of testicular steroidogenesis.

Animals↗
PubMed1985

[Bronchogenic cysts of the mediastinum in children. Apropos of 14 cases].

Fourteen children, aged from 2 months to 14 years, underwent surgical excision of a bronchogenic cyst in the mediastinum. Nine of these children were asymptomatic and the finding of the tumor was purely accidental. Retrotracheal and subcarinal localizations were more often symptomatic but their visualization on standard chest roentgenograms was sometimes difficult. An oesophagogram was helpful for the diagnosis in those circumstances. No postoperative complication occurred in our series.

Adolescent↗
PubMed1984

Intra-arterial infusion chemotherapy in rectal cancer.

Chemotherapy by intra-arterial infusion, using mitomycin C and 5-fluorouracil, was administered to six patients with cancer of the rectum. In three patients who presented initially with an inoperable rectal tumour, medication was infused through the inferior mesenteric artery. Tumour regression was documented in all three, allowing subsequent resection in two. Of three other patients with locally recurrent rectal cancer, none showed tumour regression after infusion, but two experienced substantial relief of perineal pain, with improvement in the quality of life. Unexpected complications occurred in the form of a reversible ischemic colitis in two patients and an arterioenteric fistula in a third.

Aged↗
PubMed1982

[Pleural mesothelioma: 3 developmental forms].

In the medical literature, there have been fewer reports on benign pleural mesotheliomas than on their malignant counterparts. We have reviewed the clinical, radiological and pathological features of mesotheliomas seen at the Hôtel-Dieu in Montreal over the past ten years. This experience leads us to postulate that there are three types of mesothelioma. The malignant form is often typical. Pedunculated lesions most often prove to be asymptomatic and benign. However, we have identified an intermediate category where the definitive nature of the mesothelioma could only be established after long term follow-up of the biological behaviour of the tumor.

Adult↗
PubMed1981

Limited in vitro activity of cefamandole against 100 beta-lactamase- and non-beta-lactamase-producing Haemophilus influenzae strains: comparison of moxalactam, chloramphenicol, and ampicillin.

In the present study, the minimal inhibitory concentrations and minimal bactericidal concentrations of moxalactam, cefamandole lithium, ampicillin, and chloramphenicol were determined, both in broth and on solid medium, against 75 non-beta-lactamase-producing and 25 beta-lactamase-producing strains of Haemophilus influenzae. Most of the 75 strains were inhibited or killed by 2 microgram or less of ampicillin, chloramphenicol, or moxalactam per ml, but cefamandole exhibited poor bactericidal activity against 11 non-beta-lactamase-producing strains, of which 9 were non-type B H. influenzae. Most of the 25 beta-lactamase-producing H. influenzae were resistant to 128 microgram of ampicillin per ml. Both moxalactam and chloramphenicol, which had minimal inhibitory concentrations of less than 0.25 and 2 microgram/ml, respectively, were more active than cefamandole, which had a minimal inhibitory concentration ranging from 2 to greater than or equal to 128 microgram/ml.

Ampicillin↗
PubMed1976

Rectal biopsy in type 4 glycogenosis. An ultrastructural cytochemical study.

Rectal biopsy material from a patient with type 4 glycogenosis was studied by ultrastructural cytochemical methods. The diagnosis of the disease was made on the basis of the patient's clinical history, the autopsy findings, and the histopathological features. Numerous large macrophages were observed in the rectal mucosa. They contained large vacuoles filled with filamentous material and small granules. This amylopectin was stained by the Thiery method (periodic acid-thiocarbohydrazide-silver proteinate) after 18 hours of exposure to thiocarbohydrazide; only 30 minutes was sufficient to demonstrate seemingly normal beta-glycogen particles in epithelial cells.

Autopsy↗