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Biomedical subjects

P Schulze

Publications and source records attributed to P Schulze.

At least 19 recordsLinked to original sources
PubMed1999

Demonstration of group C rotaviruses in fecal samples of diarrheic dogs in Germany.

Three out of 9 fecal samples from diarrheic dogs, which were positive for rotavirus by electron microscopy, revealed RNA-migration patterns identical to those of porcine group C rotavirus when studied by polyacrylamide gel electrophoresis (PAGE). The results were further confirmed by solid phase immuno electron microscopy (SPIEM). This is the first report of the occurrence of group C rotaviruses in dogs.

Animals↗
PubMed1999

Investigation of skin samples from red foxes (Vulpes vulpes) in eastern Brandenburg (Germany) for the detection of Borrelia burgdorferi s. l.

During earlier investigations a high prevalence of Borrelia (B.) burgdorferi s. l. in unfed Ixodes (I.) ricinus ticks in the Federal State of Brandenburg has been demonstrated. In the present study skin samples were obtained from 100 red foxes (Vulpes vulpes) from the districts where the highest B. burgdorferi prevalences had previously been found (i.e. Uckermark, Barnim, Märkisch-Oderland, Oder-Spree). BSK- and MKP-medium including inhibitory substances were used for cultivation of spirochaetes. Non-motile spirochaete-like organisms were observed in 26% of the samples. Additionally, by subcultures it was not possible to obtain motile helical forms characteristic for B. burgdorferi. On tryptose agar, the bacteria which produced nonmotile forms appeared as corynebacterium-like-colonies. Investigations by electron microscopy showed that the immobile spiral forms were giant whips (flagellae) which belonged to the contaminant flora. These forms proved to be negative for B. burgdorferi s. l. by the use of a nested-PCR. In a further study, the same skin samples were investigated for the presence of B. burgdorferi s. l.-DNA using a nested-PCR. Seven out of 100 samples were positive.

Animals↗
PubMed1998

[Dermatomycosis caused by Trichophyton verrucosum in mother and child].

In recent years, there has been an epidemiological renaissance of zoophilic dermatophytoses caused by a variety of factors. At present, the most important causative organisms are Microsporum canis, Trichophyton mentagrophytes var. granulosum and, as in the present case, Trichophyton verrucosum. These are formerly notifiable pathogens which are highly virulent and contagious. The example of an extensive, originally unrecognized tinea corporis et faciei in mother and child presented here shows the current importance of Trichophyton verrucosum, but also the diagnostic difficulties in dealing with a formerly rare infection disease. The inflammatory symptoms of deep trichophytosis with imminent danger of scar formation was the basis of synergistic combination therapy in the two patients. The source of infection for zoophilic dermatomycosis at the beginning of the epidemiological increase were looked for almost exclusively in Mediterranean countries. However, there are now increased indications of indigenous pools. In view of the neglect of consistent immunization of livestock and the lack of a requirement to notify the disease, a further rise in the number of cases in humans is to be expected.

Adult↗
PubMed1998

Hypocomplementaemic urticarial vasculitis: successful treatment with cyclophosphamide-dexamethasone pulse therapy.

Systemic hypocomplementaemic urticarial vasculitis unresponsive to several immunosuppressive and immunomodulatory drugs was seen in two women aged 43 and 45 years. Cyclophosphamide-dexamethasone pulse therapy was started in both patients and resulted in significant clinical improvement. The pulse treatment was well tolerated in both patients and no major adverse effects occurred. These cases indicate that cyclophosphamide-dexamethasone pulse therapy is efficient in the treatment of hypocomplementaemic urticarial vasculitis.

Adult↗
PubMed1997

[Use of a fast test to detect rotavirus in feces].

The commercially available immunoassay "OnSite Rotavirus" was used for the detection of animal rotaviruses in 113 faecal samples. The sensitivity of the test was 88% and the specificity 96% compared with reference methods (EIA, EM). This test would detect approximately 4.4 x 10(6) to 1.8 x 10(7) virus particles per ml. The presence of virus could be demonstrated in fresh faecal samples from cattle, horses and pigs within a few minutes. The rotaviruses of group A were identified independently of the virus serotype. Further results and additional problems of using this test kit are described.

Animals↗
PubMed1997

[Legal principles in criminal and regulatory infringements-- proceedings of the view of the public prosecutor].

In legal proceedings referring to animal welfare, an effective collaboration between Veterinary officials, public prosecutor and police authorities is of eminent significance. Intensive communication among all authorities involved helps to solve the relevant facts and thus leads to a just ruling. Only by this way an effective prosecution of infringements and criminal proceedings will be ensured.

Animal Rights↗
PubMed1996

[Severe course of ornithosis and its intensive care and diagnostic problems--a case report].

Ornithosis is still a life-threatening illness unless diagnosed early and adequately treated with antibiotics. Diagnosis, however, is difficult because often no direct contact with birds is obvious. We report on the course of a severe case of ornithosis in a 45-year-old man who had no previous health problems. Infection with Chlamydia psittaci probably occurred by inhaling of contaminated dust produced by flocks of wild pigeons. A special feature of the case is that commonly used serological tests (complement fixation reaction, CFR; ELISA) failed to yield clear results. Since propagation of Chlamydia psittaci requires special biological laboratory containment facility no. 3, we were able to detect the pathogen in faeces, blood, and urine using the direct immunofluorescence antibody technique (DIFT) and a modified peroxidase antiperoxidase technique (PAP). A lot of Chlamydia psittaci was found. These results were verified by electron microscopy. As a result, the treatment could be quickly adjusted to the application of doxycyclin, which led to a rapid improvement in the patient's condition. This direct detection of Chlamydia psittaci by DIFT, the PAP technique or electron microscopy, also in faeces, blood and urine specimens, represents a useful addition to the usual microbiological methods for the diagnosis of this pathogen.

Anti-Bacterial Agents↗
PubMed1995

[Endomycoses--a problem in the diagnosis of Chlamydia psittaci infections in chickens].

Two hens, which died at an age of 42 respectively 45 weeks, showed firstly lesions of endomycosis as sole cause of illness or death at necropsy and in light microscopic investigations. With peroxidase-antiperoxidase-(PAP-)technique and in electron microscopic investigations, however, Chlamydia psittaci were also found in very large numbers in both chickens, and occurred here particularly in lungs, liver, kidneys, spleen, myocard and ovary. The results showed, that endomycosis and chlamydial infections can occur in chickens simultaneously. In such cases of mixed infections the PAP-technique may be very helpful for rapid and reliable detection of Chlamydia psittaci in formalin-fixed and paraffin-embedded chicken tissues.

Animals↗
PubMed1990

[Predictive value of electron microscopy studies in virological diagnosis].

The negative staining techniques in transmission electron microscopy have been used as a routine tool in viral diagnosis since 30 years. In a short review, different preparation techniques, fields of application, advantages and disadvantages of electron microscopy in rapid diagnostic are discussed. The application of electron microscopy depends on availability of other more effective methods. Some viruses, specially in faecal samples, are identifiable only by electron microscopy till now. Sensitivity and specificity are improved by immune electron microscopy comprising suspension, immunosorbent and immunogold techniques. Using rapid embedding techniques, ultrahistology is a valuable tool in identifying viruses in biopsy samples, too.

Histological Techniques↗
PubMed1989

Atypical human rotaviruses in the G.D.R.

Following the aetiologic agent of diarrhoea in children aged one to three years we found in the stools a large number of rotaviruses by electron microscopy (EM), although ELISA was negative; the sensitivity of ELISA had been confirmed in previous investigations. In 141 children with diarrhoea 10 conventional (group A) and 21 antigenically distinct rotaviruses were detected. The presence of atypical rotaviruses could be confirmed by electrophoretic analysis of their RNA migration profiles. The electropherotype pattern corresponded to the group C rotaviruses reported by others.

Antigens, Viral↗
PubMed1989

[Erythema elevatum diutinum--a rare variant of skin changes in systemic lupus erythematosus].

It is reported on 5 patients with erythema elevatum diutinum, in 2 of these cases exists a systemic lupus erythematosus and another patient suffers from cutaneous lupus erythematosus. This information can be considered as a possibility that cutaneous alterations similar to erythema elevatum diutinum could be the first symptoms of systemic lupus erythematosus when the usual treatment is inefficient.

Adult↗
PubMed1989

[Multicenter reticulohistiocytosis. A contribution to clinical aspects and therapy].

It is reported on a 48-year-old woman suffering from a multicentric reticulohistiocytosis. Most trouble in this case is caused by severe arthritis, which could not be stopped by various therapy schedules. The actual treatment is the combination of Prednisolone, Chloroquine, Ibuprofen and roentgenotherapy of the hands.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1988

Unusual mitochondrial reaction in psoriatic keratinocytes.

Electron microscopic investigation of skin biopsies revealed that keratinocytes in clinically uninvolved skin from psoriatic patients show the same mitochondrial ring phenomenon after the application of dithranol as has been observed in the cutaneous lesions proper. In various other skin diseases no mitochondrial reaction was evident after dithranol treatment, nor was it seen in chronic dermatitis when dithranol was not applied. It appears, therefore, that this morphological phenomenon is specific to psoriasis and may provide information concerning functional alterations in the pathogenesis of this disease.

Adult↗