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Biomedical subjects

P Saukko

Publications and source records attributed to P Saukko.

At least 37 records · Page 2Linked to original sources
PubMed1989

Evaluation of eosin-fluorescence in the diagnosis of sudden cardiac death.

The diagnostic significance of the eosin-fluorescence method was evaluated and compared with the enzymehistochemical beta-hydroxybutyrate-dehydrogenase method (beta-HBDH) and the degree of hyperchromasia in 568 samples from 24 bets-HBDH-negative and beta-HBDH-positive sudden cardiac deaths (SCD) and 23 non-cardiac deaths as controls. The effect of autolysis was investigated separately on isolated human hearts at +4 degrees C and +22 degrees C. All samples were examined without any knowledge of clinical or autopsy data. Normal olive-green fluorescence was observed in only five individuals of the control group and two in the beta-HBDH-negative one. The frequency of the yellow-fluorescence and hyperchromasia increased from the control subjects towards the beta-HBDH-positive-ones. The difference in frequency distribution of fluorescence between the groups was statistically highly significant (P less than 0.001). The change in fluorescence did not correlate with the loss of beta-HBDH-reaction or the distribution pattern of hyperchromasia. Postmortem autolysis did not change the fluorescence significantly but the 78% wrong positive subjects in the control group render this method too sensitive and unreliable for medicolegal purposes.

Adult↗
PubMed1987

Oxidative enzyme activities and respective histochemical reactions in ischemic rat myocardium.

To evaluate the diagnostic role of histochemically demonstrated aerobic dehydrogenases in ischemic myocardial injury NADH-diaphorase, succinate dehydrogenase (SDH), beta-hydroxybutyrate dehydrogenase (HBDH) and malate dehydrogenase (MDH) were demonstrated histochemically and the corresponding enzyme activities were measured biochemically in isolated perfused rats hearts after global ischemia from 0 to 12 h. The present data show that the enzyme-histochemical methods when used properly are more sensitive indicators of early ischemic injury than classical histological staining procedures. From the enzymes tested here the histochemical demonstration of HBDH turned out to be best suited for use when suspecting ischemic myocardial injury at autopsy.

Animals↗
PubMed1987

The effect of ethanol with a meal on the mucosa of the stomach and duodenal bulb.

The effect of ethanol together with a meal on the mucosa of the stomach and duodenal bulb was studied in nine healthy volunteers. The pre-study gastroscopy was performed after an overnight fast. Two to four weeks later the volunteers drank 72-136 g (mean 111.2 +/- 18.3 SD) alcohol together with a meal. They underwent endoscopy again on the following morning. The endoscopic and histological changes were evaluated by scoring the lesions (0-4). Alcohol caused slight to moderate endoscopically detected changes in the stomach of seven volunteers, the difference being statistically almost significant (p congruent to 0.05) in the case of haemorrhagic lesions. In two subjects slight hyperaemia was seen in the duodenal bulb. The histological study did not reveal significant changes after alcohol drinking. The bile acid concentrations of the gastric juice did not change after drinking. It is concluded that ethanol with a meal causes only slight changes in the mucosa of the stomach, haemorrhagic ones being the most prominent.

Adult↗
PubMed1985

[Iatrogenic hemorrhagic complications in gynecologic laparoscopies--report of 2 cases with a fatal course].

Endoscopy and laparoscopy are part of modern gynecology. Using them, however, implies the occurrence of certain complications. The rate of complications, according to international statistics, about 3.5 per thousand with a lethality rate of 0.02 per thousand, is significantly influenced by the practitioner's personal experience and skill. The most relevant complication is hemorrhage. As a result of the patient's position, the aorta and pelvic arteries are most endangered. The symptoms of complications should be kept in mind by the physician, as well as the methods of rendering immediate medical support. Two cases with a fatal outcome are illustrated, which were caused by ignorance of an acute and slow hemorrhage, respectively. Publishing them could help to prevent further such cases.

Adult↗
PubMed1985

Correlations between enzyme histochemical reactions and respective enzyme activities in global ischaemic rat hearts.

NADH-diaphorase, succinate dehydrogenase (SDH), beta-hydroxybutyrate dehydrogenase (beta-HBDH), malate dehydrogenase (MDH) and cytochrome oxidase (CytO) were demonstrated histochemically in isolated perfused rat hearts during global ischaemia from 0 to 12 hours. The corresponding enzyme activities were measured when possible. The histochemically demonstrable activities of NADH-diaphorase and MDH decreased during the first hour of ischaemia. The time course of inactivation of biochemically detectable NADH-ferricyanide oxidoreductase was much the same as that of NADH-diaphorase. Both histochemically and biochemically detectable beta-HBDH gradually decreased by about 6 h of ischaemia. NADH-diaphorase but not MDH itself proved to be the rate-limiting factor when demonstrating MDH histochemically with nitroblue tetrazolium (NBT), whereas in the case of beta-HBDH the situation was probably the reverse. CytO and SDH activities did not change during the experimental period. Histochemistry clearly demonstrated ischaemic cellular injury, even though no significant diagnostic changes of ischaemia were visible by light microscopy. Even though this shows that enzyme-histochemical methods can be sensitive indicators of early ischaemic injury, in practice the time between the onset of injury and death as well as between death and autopsy must be taken into consideration when interpreting the results.

Animals↗
PubMed1984

The hypotonic hemolysis and the protective action of lysophosphatidylcholine.

Lysophosphatidylcholine (LPC) protected against hypotonic hemolysis during rapid swelling of erythrocytes in spherocytic concentrations without any increase in the maximal cell volume. The total prelytic potassium release was increased, but the prelytic potassium release per cell was the same or decreased in the presence of LPC. The comparison of measured and calculated cell volume increase suggested that the prelytic potassium release must have been connected with a considerable sodium influx suggesting serious damage of the cell membrane already during swelling in the absence of LPC. Gradual swelling shifted hemolysis to more dilute solutions and LPC did not further increase the shift. We suggest that LPC protects the erythrocytes against hypotonic hemolysis during rapid swelling via a prevention of the initial membrane damage through altering the interaction between lipid bilayer and membrane cytoskeleton in the same way as occurs during incubation in a medium with low ionic strength.

Dose-Response Relationship, Drug↗
PubMed1983

Effects of glutaraldehyde and critical point drying on the shape and size of erythrocytes in isotonic and hypotonic media.

Scanning electron microscopy (SEM) was applied to studies on osmotically swollen erythrocytes fixed by a method using increasing concentrations of glutaraldehyde (GA), OsO4 as a post fixative, and critical point drying (CPD). This fixation method prevented the osmotic effect of GA itself and preserved the cellular equilibrium shape intact in various media, i.e. discocytes in an isotonic medium and ellipsoids or spheres and ghosts in a hypotonic medium. The discocytes were reduced less in diameter and area than the ellipsoids and spheres during CPD, but the degree of volume shrinkage was of the same magnitude (approx. 73%) regardless of the cell shape. The shrinkage of the discocytes was advanced until the dry volume (approx. 29 microm3) was reached, while the shrinkage of the osmotically swollen cells ceased earlier. A miniaturization process coupled with solvent evaporation is suggested as an explanation for this. The osmotic swelling of erythrocytes was studied quantitatively with the aid of SEM micrographs. The increase in volume was about 60%, which is in agreement with the values obtained by light microscopy for unfixed and GA-fixed cells in the present work and with those reported in the literature.

Aldehydes↗
PubMed1983

Influence of ethanol on systemic and pulmonary hemodynamics in anesthetized dogs.

Immediate circulatory reactions to acute intragastric ethanol administration were studied by a catheterization technique in spontaneously breathing dogs. Diluted ethanol was given in a dosage of 1 g/kg in test group I (n = 11), and 2/kg in group II (n = 10). The control group (m = 14) received only water. The highest blood ethanol concentration was 0.90 +/- 0.07 mg/ml (mean +/- SE) in group I, and 1.97 +/- 0.10 mg/ml in group II. Heart rate and cardiac output increased (p less than 0.,001), but stroke volume, mean aortic blood pressure and right atrial blood pressure remained practically unchanged. Systemic vascular resistance decreased. Mean pulmonary arterial blood pressure increased markedly in both test groups (p less than 0.001) while pulmonary arterial wedge pressure did not change. The pulmonary arterial resistance increased (p less than 0.01). Changes in respiratory rate or volume and arterial pO2 were negligible in group I, but respiratory minute volume decreased in group II. In conclusion, ethanol in concentrations 0.5 to 2.0 mg/ml increased resistance in the pulmonary arterial tree, indicating pulmonary arterial vasoconstriction, but reduced systemic vascular resistance, thus putting a concept of peripheral vasodilation in favour.

Animals↗
PubMed1981

Propranolol and sotalol metabolism after a drinking party.

The effects of alcohol consumed during an evening party on the metabolism and response of beta-adrenergic blocking drugs were investigated in healthy subjects. The drugs were given 12 hr after the first drink. The plasma clearance rate of propranolol, metabolized in the liver, increased, while that of sotalol, which is eliminated unchanged, was reduced. Plasma propranolol levels, but not those of sotalol, were related to blood alcohol content. The blood pressure-reducing effect of propranolol diminished after alcohol and that of sotalol increased. Both drugs reduced the heart rate after alcohol although they were unable to cancel out entirely the alcohol-induced increase in the heart rate. The results show that drinking may alter the metabolism of beta-blocking drugs. Drinking habits must be considered in therapy with beta-blocking drugs or when seeking reasons for angina pectoris or arterial hypertension that does not respond to therapy.

Adult↗
PubMed1980

Changes in the myocardium and skeletal muscle in guinea pigs in cold exposure with and without ethanol.

The effect of hypothermia with and without ethanol on the myocardium and skeletal muscle was studied. Changes were observed in both muscle types. The mildest lesions were discoloration of the muscle cells with acid fuchsin and Heidenhain's iron haematoxylin staining, these being more marked in the skeletal muscle. Waving and contraction bands in the muscle were seen in hypothermia. The most severe lesion was a focus with oedema and haemorrhage, a reduced reaction of beta-hydroxybutyrate dehydrogenase and fragmentation of the muscle cells, and this was more frequent in the myocardium. Occasionally discoloration, contraction bands and waving were also seen in the controls killed by a blow on the neck. The changes were more numerous in the guinea pigs given ethanol before cold exposure, and serum creatinine phosphokinase was elevated in the same group. Urinary excretion of adrenaline increased in cold exposure, but noradrenaline did not change significantly. Hypoxia, catecholamines, and sludging of the blood are discussed as possible aetiological factors for the lesions.

Alcohol Drinking↗
PubMed1980

Metabolic effects of a low-magnesium diet in pigs.

1. Pigs were fed on semi-purified food. The magnesium content of the experimental diet was 1.0 and that of the control diet 1.9 g/kg. 2. In the low-Mg group serum triglycerides and blood lactate values were increased and base excess and standard bicarbonate values were decreased, indicating metabolic acidosis. 3. A significant positive dependence was found between blood pH and serum Mg:Ca value as well as between blood pH and serum Mg, and also between body temperature and blood lactate values in the low-Mg group. None of these dependences was significant in the control group with magesium acetate supplementation in the food. 4. No specific histopathological changes were found in heart, liver or kidney of the experimental animals. 5. The results indicate that an increase in serum triglycerides does not need to depend on the amount of quality of food fat. 6. The present study shows that, in pigs, a low-Mg diet may cause metabolic disturbances in instances with the food Mg content is distinctly higher than the normal values recommended by the (UK) Agricultural Research Council (1966) and (US) National Research Council (1968).

Animals↗
PubMedDate not supplied

The acute dose-related effects of ethanol on right ventricular function in anesthetized dogs.

The acute dose-related effects of small to moderate doses of ethanol on right ventricular functioning were studied on 18 anesthetized, artificially ventilated dogs in 39 sessions. Diluted ethanol (from 25-37.5%) was infused during 40 minutes, yielding total doses of 1.0 g/kg (n = 15), and 1.5 g/kg (n = 12) with corresponding venous blood ethanol peak concentrations of 1.38 +/- 0.25 and 2.41 +/- 0.31 mg/ml, respectively. Heart rate increased up to 16% in groups receiving ethanol. In the control group receiving the equivalent volume of saline (n = 12) heart rate decreased 14%. Pulmonary arterial systolic pressure increased from 24 +/- 3 to 27 +/- 3 mmHg and diastolic pressure from 11 +/- 2 to 14 +/- 4 mmHg (p less than 0.05) when the ethanol dose was 1.0 g/kg. The pulmonary arterial resistance increased from 620 +/- 135 to 805 +/- 185 dyn.s.cm-5 (p less than 0.01). The peak dP/dt decreased maximally by 20% with increasing ethanol doses. Stroke volume decreased maximally by 14% but due to the increase in heart rate, cardiac output even increased. The changes in end-diastolic volume and pressure were not significant. Hence, the ethanol increased heart rate and afterload of the right ventricle but depressed the myocardium.

Animals↗
PubMedDate not supplied

The acute dose-dependent effects of ethanol on canine myocardial perfusion.

The acute effects of ethanol (1.0 g/kg and 1.5 g/kg, n = 4 and n = 5, yielding blood concentrations of 1.3 +/- 0.2 mg/ml and 2.4 +/- 0.3 mg/ml) on myocardial perfusion were studied in anesthetized, thoracotomized, artificially ventilated dogs by using a radioactive microsphere technique. The control group (n = 5) received saline. The smaller dose of ethanol decreased perfusion in the left ventricular myocardium from 0.737 +/- 0.122 to 0.555 +/- 0.122 ml/g/min (NS), whereas the greater dose nonsignificantly increased it, from 0.744 +/- 0.115 to 0.819 +/- 0.119 ml/g/min (p < 0.01 between the groups). These changes were most evident in subendocardial layers (p < 0.01 both within the groups and between the groups). The greater dose of ethanol increased systemic vascular resistance (p < 0.01 when compared to the control group). The changes in right ventricular myocardium were insignificant. The acute effect of ethanol on coronary blood flow is dose-dependent with small to moderate doses reducing demand for left ventricular flow but with increasing doses evoking not only an increase in demand for flow but also an increase in flow.

Animals↗
PubMedDate not supplied

Influence of ethanol on circulation in surface-induced hypothermia and subsequent rewarming.

Hypothermia and ethanol are often closely linked and in hypothermic accidents ethanol is often a contributing factor. To study the effects of ethanol on the circulation in hypothermic conditions, cardiac catheterization was carried out on 18 anaesthetized beagle dogs. They were divided into two groups. One gram of ethanol/kg of b.wt. diluted in saline was infused into the vena cava superior within 30 min to seven dogs. The dogs were then cooled between ice bags until the blood temperature in the ascending aorta was 25 degrees C and they were then rewarmed. The control group of 11 dogs was cooled and rewarmed without ethanol infusion. The heart rate first increased when cooling down to 33 degrees C and decreased thereafter in the control group. In the ethanol group heart rate increased during the ethanol infusion and remained high when cooling down to 33 degrees C and decreased thereafter. Heart rate was higher in the ethanol group throughout the experiments, and during rewarming the difference was significant. In the control group cardiac output first increased until a body temperature of 33 degrees C was achieved but then decreased. In the ethanol group cardiac output started to decrease after ethanol infusion. During rewarming there was a significantly higher cardiac output in the ethanol group, probably due to the higher heart rate. In the cardiac cycle the systolic period prolonged significantly (p < 0.001) in both groups when the body temperature decreased from 37 degrees C to 25 degrees C whereas the diastolic period remained quite stable. The contraction phase was also affected by the cooling. The changes in contraction force cannot be seen in dP/dt alone because dP/dt values first increased significantly when cooling from 37 degrees C to 33 degrees C but then decreased. Ejection fraction, systolic period, and the systemic vascular resistance increased despite the reduction of the dP/dt and thus we conclude that the contraction force is augmented in hypothermia. In the ethanol group the myocardium seems to be depressed due to ethanol. In the early phase of cooling heart rate increased but cardiac output decreased in the ethanol group, indicating the decreased ability of the heart to respond to cooling in the presence of ethanol. The time constant of exponential pressure fall (tau) increased linearly with cooling from 37 degrees C to 25 degrees C and recovered with rewarming in both groups. Changes in negative dP/dt coincided with the changes in the time constant of exponential isovolumic pressure fall. Ethanol did not influence relaxation. All the parameters we checked recovered to normal during rewarming.

Animals↗