Search PubMed⌕ Search

Biomedical subjects

P Roux

Publications and source records attributed to P Roux.

At least 181 records · Page 10Linked to original sources
PubMedDate not supplied

A common antigenic epitope expressed on human Pneumocystis carinii.

Recently antigenic heterogeneity in human Pneumocystis carinii (Pc) isolates was observed in several laboratories. Monoclonal antibodies (MAb) were produced to human Pc (PcH) from a lung autopsy sample from a non-AIDS patient (MAb Group I, n = 10), or from bronchoalveolar lavage (BAL) fluid from AIDS patients (MAb Group II, n = 8). To detect Pc antigen from specimens, indirect immunofluorescence and immunoblotting techniques were used. The reactivity was evaluated by using one autopsy sample from the non-AIDS patient and 14 BAL samples from AIDS patients. The MAb in group I (C5-9, E9) stained a part of PcH from all isolates. On the other hand, several MAb in group II (L20-5, M34-2, M78-3, M79-5, N23-4) stained all PcH from all isolates. Some MAb (C5-9, E9, M34-2, M78-3) stained cysts as well as trophozoites. Immunoblot studies detected a 92 kDa molecule as a common antigen by all of these MAb. Therefore, we have found a common antigenic epitope on PcH and MAb that recognize this epitope may become useful for diagnosis of infection and for biological characterization studies on the organism.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Attempts at in vitro cultivation of Pneumocystis carinii from human bronchoalveolar lavage fluids without feeder cells.

We attempted to cultivate Pneumocystis carinii obtained from two bronchoalveolar lavage fluids of AIDS patients with P. carinii pneumonia, in a system wherein cysteine and 2-mercaptoethanol were substituted for the feeder cells. The presence of P. carinii cysts was monitored for 11 days under conditions of continuous culture. Moderate increase in cyst forms was observed until day 11. Further study with this system would be required to determine if the observed increase in cyst numbers is reproducible and whether the cyst form is a response to adverse in vitro conditions or is a manifestation of growth.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Value of an immunofluorescence assay for the detection of Pneumocystis carinii in Bronchoalveolar lavage.

An indirect immunofluorescence monoclonal antibody assay was found to have higher sensitivity than usual stains for the detection of Pneumocystis carinii, particularly in bronchoalveolar lavage fluids in which there are only a few parasites, as in HIV-patients or in HIV+ patients with prophylaxis or treatment. For patients without any therapy, when different stains give conflicting results, decisions on therapeutic approaches to be used should consider the patient's clinical and biological status. Prospective studies are necessary to evaluate the predictive value of low parasitism in asymptomatic immunosuppressed patients.

Bronchoalveolar Lavage Fluid↗
PubMedDate not supplied

Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence.

The polymerase chain reaction (PCR) using published primers and probes has been compared to conventional stains and immunofluorescence for diagnosis of Pneumocystis carinii. We have screened 71 bronchoalveolar lavage (BAL) fluids from HIV-immunosuppressed patients. Of 34 samples negative by conventional stains and immunofluorescence, only one was positive by PCR. Thirty of 35 samples positive by conventional stains and immunofluorescence were also positive by PCR. One BAL sample, negative by conventional stains but positive by immunofluorescence, was negative by PCR. These data are discussed in relation to clinical and therapeutic conditions of the patients.

Bronchoalveolar Lavage Fluid↗
PubMedDate not supplied

[Pulmonary bilharziasis caused by Schistosoma mansoni with impairment of general state].

The authors report on one case of pulmonary bilharziosis at Schistosoma mansoni observed in a West-Indian female showing an impairing of her general condition with fever and dyspnea. Diagnosis was brought out by a pulmonary biopsy, after thoracotomy. Treatment by praziquantel was remarkably efficient. Scarcity of symptomatic cases and paucity of pulmonary radiological pictures are opposite of the frequency of pathological lesions in this parenchyma. The authors discuss the physiopathology of pulmonary bilharziosis in both their invading phase and state phase, as well as their frequency. They come to the conclusion that both parasitological examination of stools and biopsy of mucous membrane of rectum are simple, reliable and harmless implements of diagnosis.

Biopsy↗
PubMedDate not supplied

[Neurologic tumors of the small intestine. Review of the literature apropos of 6 personal cases].

In connection with five cases of benign neurogenic intestinal tumors (3 solitary schwannomas, 1 schwannoma associated with a gastric schwannoma without von Recklinghausen's disease, 1 neurofibroma associated with von Recklinghausen's disease) and one malignant neurogenic tumor, the authors review the general and radiological features of these rare lesions. From a diagnostic standpoint, in view of the warning sign constituted by hemorrhage in the absence of von Recklinghausen's disease, angiography remains the most effective radiological technique. During von Recklinghausen's disease, lesions are usually small and undetectable by X-ray due to their intramural or subserosal situation. Even when radiological techniques allow a diagnosis to be made, a complete investigation of the entire digestive tract is advisable to determine whether the intestinal lesion detected is indeed solitary and responsible for any symptoms.

Adult↗
PubMedDate not supplied

[Role of scanography in the diagnosis of parathyroid adenomas. Apropos of 27 cases].

Scanner imagine pre-operatively in 27 patients with hyperparathyroidism provided assessment of the sensitivity (92 p.cent) and diagnostic precision (92.5 p.cent) of this investigational method. The need for a strict methodology and the advantages gained by high resolution and angioscanning programmes are emphasized. After analyzing the results, a review of the published literature enables the advantages and the limits of the scanner X to be defined, and a strategy for exploration of parathyroid adenomas to be proposed.

Adenoma↗
PubMedDate not supplied

[Congenital cysts of the liver in echography].

The authors present 150 cases of congenital biliary cystic pathologies of the liver: solitary congenital cyst (98 cases), multiple congenital cysts (40 cases), hepatic polycystosis (8 cases), and Caroli's disease (4 cases). The general characteristics of these congenital liver affections are reviewed, including discussion of their frequency, classification and potential for evolution. The problems raised by this type of pathology and the benefits of ultrasonography are emphasized.

Adolescent↗
PubMedDate not supplied

[Critical evaluation of non-biological complementary examinations of the biliary tract before deciding on therapy (author's transl)].

A critical evaluation of non-biological complementary examinations of the biliary tract was conducted to assess their value when deciding on therapy. The published literature was reviewed, and the sensitivity and specificity of the different methods presented, emphasis being placed on the primary investigations of ultrasonography and oral and intravenous biligraphy. Secondary exploratory procedures (transparietal cholangiography, retrograde endoscopy with cholangiography and pancreatography, scintigraphy, gallbladder infusion parietography, scanography, and arteriography) are defined and their value discussed. Published data suggest that ultrasonography should be the initial investigation, this often being sufficient to enable suitable therapy to be chosen. Only approximately 5 p. cent of patients are unable to benefit from gallbladder exploration, and these require conventional radiological examinations before deciding on therapy.

Biliary Tract↗