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Biomedical subjects

P Rossmann

Publications and source records attributed to P Rossmann.

At least 91 records · Page 5Linked to original sources
PubMed1979

The effect of native and sonicated double-stranded polyribonucleotides on the course of spontaneous autoimmune disease in NZB and NZB/Swiss F1 mice.

The administration of sonicated fractions of f2 phage polyribonucleotides caused an increased weight loss and deterioration of the clinical state in female NZB mice. Discontinuance of the treatment resulted in an improvement of both the clinical state and the genetically determined autoimmune disorders of these mice. Some potential explanations of this effect are discussed.

Animals↗
PubMed1978

Miliary tuberculosis in a patient after renal transplantation.

A report is presented on the first case of miliary tuberculosis in a group of 84 kidney transplant recipients treated in the Institute of Clinical and Experimental Medicine, Prague, until March 1976. The specific process was verified by bacteriological investigations of the sputum and urine. Productive specific tuberculoid-type nodules with sporadically occurring acidoresistant rods were detected in a bioptic specimen of the transplanted kidney. Also discussed are therapeutical problems in progressive renal graft insufficiency and in simultaneous chronic hepatopathy.

Adult↗
PubMed1978

The effect of mercury derivatives of fluorescencein on allotransplant kidney in experiment.

Mercurascan (MSC), a mercury derivative of fluorescein, was studied as to its effect on the survival of allogeneic kidney grafts in dogs. A single perfusion of the donor's kidney with saline solution with MSC added in a ratio of 1 mg/100 ml was found to have significantly extended the graft survival time from 10.8 days (SE +/- 0.6) to 15.4 days (SE +/- 1.6). Graft survival time extension proved to be even more significant after a single dose of MSC given to the donor (0.5 mg/kg b.w.) and repeated administration of an equal MSC dose to the recipient twice weekly. Listed are detailed morphological changes in tissue specimens from the kidney, lymph nodes, spleen, and many other organs of a dog surviving for 42 days. A very moderate rejection lesion with merely tiny lymphocytic infiltrates was proved in the kidney. Lymph node structure was almost entirely obliterated with lymphoid tissue extinction. There was loss of white pulp in the spleen. The absence of morphological changes in the other organs suggested good tolerance of the preparation. More studies are called for to elucidate the complex MSC intervention in the process of rejection.

Animals↗
PubMed1976

Clinical detection of rejection nephropathy using 125I-labelled fibrinogen.

Using a modified fibrinogen-uptake (FUT) test, 22 patients were investigated at various intervals after kidney transplantation. Eight of the patients had developed or showed early clinical signs of acute rejection crisis at the time of measurement. Another fourteen patients formed the control group with no evidence of florid rejection process. Comparison of the 125I-fibrinogen accumulation in the renal grafts between the two mentioned groups showed clear differences with high statistical significance (p less than 0.001). Uptake of labelled fibrinogen was increased in every acute rejection transplant. Histologically the kidneys with increased accumulation of fibrinogen showed extensive deposits of fibrin in blood vessels, glomeruli, intracapillary thrombi and in the interstitium. Except for the limitations discussed in this paper we consider this test to be of a great clinical value in the diagnosis of rejection episodes.

Female↗
PubMed1975

Experimental immune complex glomerulopathy: immunomorphological aspects and correlations with human disease.

Thirteen rabbits received parenteral injections of human serum albumin (HSA) in regular doses and intervals during 6-32 seeks. The antigen load elicited immune responses of variable degree, giving a good correlation with the extent and quality of immune complex (IMC) deposition, as shown by immunological methods, electron and fluorescence microscopy and by histoautoradiography. Morphology of immune deposits (IMD) depended on the composition of the IMC and produced a continuous scale of glomerular involvement, ranging from minimal and focal to massive and diffuse deposition. Probable rudimentary traces of IMD were apparent in the ultrastructure even in animals with negative results of both immunological and immunfluorescence examinations. The relationships are discussed between experimental IMC-induced glomerulopathy on the one hand, and some problems of development and bioptic evaluation of human glomerulonephritis.

Animals↗
PubMed1975

Glomerulopathies in human renal allografts.

The present study discusses the light, electron and immunofluorescence microscopy as well as some clinicopathologic correlations of rejection change in human renal allograft glomeruli. It is based on examination of 126 tissue specimens from 54 grafts obtained from 50 patients (1966-1973). The most frequent and characteristic lesion was membranous transplant glomerulopathy (MG) with irregular fibrillar thickening of capillary walls but without conspicuous hypercellularity. This thickening was caused by subendothelial depositsdifferent from classical fibrinoid lesions. During further progression, widening and peripheral extension of mesangium with degenerative changes became apparent. Advanced MG was encountered most frequently in the 2nd year after transplantation (TPL) at moderate to medium proteinuria and hypertension. It was accompanied by endarteristic rejection changes, and renal insufficiency set on usually in the course of the 3rd year. Nevertheless, the course, symptoms, and graft survival exhibited considerable variations. - The morphology and manifestations of destructive segmental transplant glomerulopathy (SG) depended on the time of its development. In the early stage (within about 3 months after TPL), the lesion was characterized by areas of fibrinoid insudation and necro(bio)sis associated with severe vascular changes, most frequently obliterative arterio(lo)pathy (OA). The ultrastructure was characterized by endothelial defects with host's polynuclear reaction and focal intravascular coagulation. The grafts thus affected failed soon, their function usually subsiding within the first trimester at a moderate, but gradually increasing proteinuria and severe persistent hypertension. The late from of destructive SG presenting as fibrohyaline obliteration of the loops with foam cells always accompanied advanced MG with severe arterial lesions. - Fluorescence microscopy revealed both linear and focal fixation of antisera, which, however had no apparent correlation with the microscopical and clinical presentations.

Basement Membrane↗
PubMed1975

[Dense deposit nephropathy: a peculiar variant of glomerulonephritis or a distinct disease entity (author's transl)].

In four renal biopsies of two patients with chronic glomerulonephritis (GN), the so-called dense deposit nephropathy (NDD) was diagnosed by means of light, electron, and immunofluorescence microscopy. In routine paraffin sections the picture approached that of the membrano-proliferative GN. In semithin sections (toluidine blue, periodic acid-Ag-methenamine) and especially in the ultrastructure there appeared extensive confluent deposits of a very dense substance, infiltrating the lamina densa of glomerular capillaries, basal membranes of both Bowman's capsules and tubules, and arteriolar walls. In this localization, a non-diffuse "psdudolinear" deposition of beta1c was detected, whereas antisera to main Ig-fractions and fibrin(ogen) were not fixed. In a biopsy performed six years later, a concentration of dense depositis towards the mesangial area and a partial regeneration of basal membranes were observed. In a part of dense deposits there appeared vacuolization, primarily in tubular and arteriolar basal membranes. In glomeruli, focal IgM deposits were apparent at an advanced stage. NDD apparently is a sequel of a particular metabolic (immune?) process, afflicting solely the renal membranous system and distinctly dns known at present. The noncharacteristic clinical presentation resembles chronic. GN, is very protracted, lengthy, and relatively benigh, with a chance of functional and possible even morphological remission.

Adult↗