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P Rossmann

Publications and source records attributed to P Rossmann.

At least 55 records · Page 3Linked to original sources
PubMed1989

[Idiopathic membranous glomerulonephritis. Clinico-morphologic relations and prognosis].

Membranous glomerulonephritis (GN) was diagnosed in 61 of 993 patients with histologically confirmed primary GN. Two-thirds of the patients were men. High hypertension was recorded in 7.5% of the patients. A typical finding was marked proteinuria (6.15 +/- 4.88 g/24 h.) with mild erythrocyturia (median 8 million in Addis sediment). At the time of biopsy 86% of the patients had normal creatininaemia, the level of which was positively correlated with the blood pressure and degree of tubulointerstitial regression. The cumulative duration of renal function in 5, 10 and 20 years was 88, 80 and 57%; during the same time intervals 22, 48 and 52% of the patients were cured.

Adult↗
PubMed1989

Detection of cationic and non-cationic markers in the rat glomerulus by electron probe analysis.

Acidic glycans (glomerular polyanion substances) in the rat kidney were visualized ultrastructurally by three cationic markers: colloidal iron, ruthenium red, and polyethylenimine-phosphotungstic acid (PEI-PTA). Heavy metal atoms (Fe, Ru and W) were detected in ultrathin sections by energy-dispersive electron probe microanalysis (EPMA). Characteristic peaks of the locally bound elements were obtained in spectra derived from the dense structures seen by transmission electron microscopy (TEM)--i.e. the glycocalyx of podocytes and/or the polyanion sites in the lamina rara externa of the glomerular basement membrane. Weaker signals were emitted by some extraglomerular structures. This finding may reflect a low concentration of glycans in structures lacking apparent density by TEM, and/or incomplete specificity of the markers, partial dislocation of reactive substances or the presence of an endogenous element (Fe). Experimental argyrosis was elicited by the peroral administration of silver nitrate. Dense Ag precipitates were seen chiefly in the lamina densa and characteristic peaks of silver were displayed in this site by EPMA, and was best demonstrated in non-contrasted sections. A single i.v. injection of Ag proteinate failed to produce glomerular pigmentation. The only dense granular product in tubular cells yielded characteristic peaks of Fe (endogenous siderosomes) but EPMA excluded detectable amounts of silver.

Animals↗
PubMed1989

[The inadequate status of information of interested lay persons about the educational campaign regarding risk factors for cardiovascular diseases].

181 interested individuals (75 males, 106 females; range 16 to 84 years) responded to a questionnaire issued by the Styrian Cardiovascular Lay Society. The main topic of the questionnaire concerned information gained via media, about the possible risk factors related to cardiovascular diseases and about any changes in life style consequent to this information. The results show that the general knowledge in this field especially in younger subjects is disappointing and that there is need for further intensive information and education.

Adolescent↗
PubMed1989

Different handling of antigen by macrophages of low-responder C57BL/10ScSn strain and high-responder A/J strain of mice. II. Antigen uptake.

The uptake of 51Cr-SRBC and 125I-ARS-BG6 in the liver and spleen of non-immune low IgG responder C57BL/10ScSn mice is higher than in the high-responder A/J strain. After immunization with SRBC, the uptake in the spleen of A/J mice is ten times higher and that in the liver three times higher than in C57BL/10ScSn mice. The higher uptake of the well responding A/J strain, which is antigen specific, is due not only to a higher level of opsonizing antibodies. Endotoxin stimulation, which increases the IgG formation of the low-responding strain, does not increase the uptake in this strain. In the low-responder strain there is a considerable level of free 51Cr in the serum and kidney within 5 min after antigen injection, suggesting a high catabolic rate of the antigen. In contrast, at that time in the A/J strain virtually all of the radioactivity is still bound to the erythrocyte fraction. Ultrastructural observations suggest that in the low-responding strain the antigen residues survived in macrophages for a long period of time. The results provide evidence of differences between the metabolic activity of macrophage populations of both strains which could be related to the low IgG response of the strain C57BL/10ScSn.

Animals↗
PubMed1988

[Status of information of interested lay persons: results of a questionnaire survey by the Styrian Heart Association].

181 interested individuals (75 males, 106 females; range 16 to 84 years) responded to a questionnaire issued within the information and instruction courses of the Styrian Cardiovascular Non-professional Society. The main topic of the questionnaire concerned information gained via media, possible risk factors leading to cardiovascular diseases and any changes in living conditions due to such facts. The results show clearly that the general knowledge in this field especially in younger subjects is disappointing and further intensive information and instruction would be urgently required.

Adolescent↗
PubMed1988

Antibody-directed affinity therapy applied to the immune system: in vivo effectiveness and limited toxicity of daunomycin conjugated to HPMA copolymers and targeting antibody.

The applicability of targeting therapy intervention in lymphatic tissue was studied. The effect was measured as the inhibition of anti-sheep red blood cell antibody response expressed in plaque-forming cells. Daunomycin was used as the effective drug and polyclonal and monoclonal anti-Thy 1.2 or anti-Iak antibody served for targeting. Both components were coupled to a soluble N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer with oligopeptidic side sequences which permitted a controlled release of the drug in the target tissue. HPMA copolymer conjugates with side sequences Gly-Phe-Leu-Gly cleavable by lysosomal enzymes decreased in vivo the antibody reaction by 60-85%. A comparable amount of free targeting antibody was without a significant effect. Injection of targeted daunomycin decreased the toxicity of the drug against hematopoietic precursors in bone marrow colony-forming unit-spleen 80 times compared to the same amount of free drug. The in vivo effectiveness of targeted daunomycin was confirmed morphologically. Application of free daunomycin lead to a significant irritation of Kupffer cells in liver while none of the daunomycin-antibody-copolymer conjugate had such an effect.

Acrylates↗
PubMed1988

Failure to detect the presence of pluripotential haemopoietic stem cells in the mouse brain.

Single cell suspensions prepared from adult mouse brains were tested for the presence of pluripotential haemopoietic stem cells (colony-forming units, CFU) by transfer into an irradiated recipient and enumeration of the CFU in the recipient's spleen. In contrast to the findings of others (Bartlett, 1982), we did not detect CFU after injection of brain cell suspensions, although they were detectable after inoculation with bone marrow cells. The number of CFU in recipients after transfer of increasing numbers of brain cells was the same as that detected in the irradiated controls which had not received any transferred cells. Finally, cells from the brain, in contrast to bone marrow cells, were not able to protect recipient animals from the effects of lethal irradiation.

Animals↗
PubMed1987

Protein aggregates in extracorporally perfused rabbit kidneys.

Eighteen rabbit kidneys were perfused ex vivo for 1 h with allogeneic blood, and in 16 a solution of xenogeneic aggregate-free, aggregated or antibody-complexed protein was added to the perfusate 5 min after the start (human immunoglobulins or serum albumin, partly cationized, were used). The kidneys were examined by light and electron microscopy and the human and rabbit immunoglobulin (or albumin) precipitates were detected by direct immunofluorescence and ultrastructural immunohistochemistry. In 13 kidneys the perfusion produced small segmental glomerular endocapillary aggregates of platelets, leukocytes, and granular precipitates reactive with both anti-rabbit and anti-human antibodies. No typical deposits were seen in mesangium or in periphery of glomerular capillaries but rabbit Ig penetrated to the inter- and subepithelial spaces of proximal convoluted tubules. Three kidneys perfused by cationized aggregated human Ig (or by cationized albumin-antialbumin complexes) exhibited a destructive lesion with rapid breakdown of blood flow and massive global endocapillary plugs of similar ultrastructure but with focal endothelial sloughing. Pericapillary granular precipitates of human and rabbit Ig were seen in these kidneys. When the blood with cationized Ig aggregates was used for perfusion of two further kidneys extensive endocapillary aggregates with endothelial damage reappeared but the extracapillary penetration and precipitation were lacking and the blood flow largely improved. Membrane polyanion of podocytes stained by colloidal iron was preserved even in close proximity of cationized complex precipitates. Thus, in the ex-vivo perfusion model the preformed neutral aggregates did not penetrate through the glomerular capillary wall and were not phagocytized by mesangial cells. The cationized aggregates induced rapid circulatory failure with massive platelet clumping and granular pericapillary "humps" ultrastructurally different from the deposits of human and experimental immune complex glomerulonephritis.

Animals↗
PubMed1986

Puromycin aminonucleoside nephropathy: ultrastructure, glomerular polyanion, and cell surface markers.

Puromycin aminonucleoside nephropathy with heavy proteinuria and oedema was induced in rats by 10 consecutive daily subcutaneous injections of aminonucleoside (1.67 mg/100 g of body weight). The main ultrastructural lesions were vacuolation of podocytes and total fusion of foot processes with loss of colloidal iron-reactive polyanion layer on the epithelial surface adjacent to the basement membrane. On the other hand the outer surface of podocytes and intravacuolar granular substance stained with colloidal iron. In scanning electron microscopy of freeze-fractured tissue the swollen podocytes and the urinary spaces displayed granular and filamentous precipitates. Seven cell surface antigens were examined by indirect enzyme immunohistochemistry with a series of MRC OX monoclonal antibodies. Glomeruli of control rats exhibited rare isolated Ia- positive endocapillary cells, possibly monocytes; these elements were significantly reduced in puromycin aminonucleoside nephropathy but there was an increase in Ia- positive cells in the cortical interstitium. Control kidneys harboured scanty interstitial T lymphocytes. These latter, especially the T8- positive cytotoxic/suppressor subpopulation, were markedly augmented in puromycin aminonucleoside nephropathy. The expression of class I histocompatibility antigens and of differentiation antigens (Thy 1) was not altered by aminonucleoside.

Animals↗
PubMed1986

Wegener's granulomatosis with bilateral necrotizing scleritis, polyarthritis and renal failure efficiently treated with immunosuppressive therapy.

A case report of a female patient with Wegener's granulomatosis is presented. After an initial involvement of the upper respiratory tract in the form of a sinusitis, there followed a severe necrotizing bilateral scleritis necessitating the enucleation of the left eye ball. Renal involvement developed as late as 24 months after the onset of the disease and led to renal failure within three months. Throughout the duration of her disease, the patient had joint symptoms in the form of episodes of migratory nondeforming polyarthritis. The administration of corticosteroids alone in daily doses up to 60 mg prednisone failed to control the progression of the disease, while immunosuppressive therapy with cyclophosphamide combined with methylprednisolone pulse therapy and haemodialysis resulted in a marked improvement of renal function and in the subsidence of the ocular and articular symptoms.

Acute Kidney Injury↗
PubMed1986

[Sleepwalking].

Explore the source record for details and available documents.

Child↗
PubMed1986

[Rejection nephropathy before and after therapeutic administration of cyclosporin A].

During 1983 to 1986 41 patients were treated with Cyclosporin A (CyA) following kidney allotransplantation (TPL). 31 received the first (29 extra- and 2 intrafamilial) graft; in 10 there was second TPL, in 9 cases under high-risk conditions, where the first graft had been destroyed by (hyper)acute rejection or by rapidly progressive rejection with early vascular lesion. 21 needle biopsies and 5 excised grafts which had been collected 5 days to 18 months after TPL were examined by light microscopy and in addition 6 of the former also underwent electron and immunofluorescence microscopic study. The glomeruli showed discrete, inconstant segmental lesions but the ultrastructure also revealed severe general endothelial swelling. The tubular system had nonspecific degenerative changes of varying extent. In 11 patients focal cytoplasmic microvacuoles appeared in proximal tubular epithelia; there were also inconstant hyaline droplets, microcalcifications, and intratubular crystals. Electron microscopy revealed multiple round dense intramitochondrial inclusions in proximal tubules. The ultrastructure of the microvacuoles resembled that of "osmotic nephropathy". The rejection infiltrate and interstitial fibrosis of various degree did not essentially differ from those of conventionally treated grafts. In 7 patients cortical arterioles and small arteries exhibited a stenosing lesion (toxic?). In 3 cases metachromatic "mucoid" thickening of intima was prominent. Ultrastructure studies showed swollen endothelial cells with numerous globular dense bodies and a severe defect in the leiomyofibrils of muscle cells of the media. Hyperplasia of juxtaglomerular apparatus was apparent in 7 patients. Immunofluorescent microscopy of two biopsies from subsequently excised grafts visualized IgM, C3, and fibrinogen in small arteries and some glomerular capillary loops. Three early nephrectomies were caused by infarct-like necrosis. The discussion deals with differences between CyA- and conventionally treated grafts, diagnostic features, interpretation of findings, and measures following biopsy. In our patients with continual CyA-treatment no case of clinically and morphologically typical obliterative arterio-arteriolopathy (OA) and rapidly progressive irreversible rejection has as yet been noted.

Biopsy↗
PubMed1985

Low IgG response of the mouse strain C57BL/10ScSn after immunization with protein antigens.

The low IgG response of the strain C57BL/10ScSn is not restricted to the reaction to sheep red blood cells; but it can be demonstrated even after immunization with ARS, DNP or FITC haptens, coupled to various heterologous (BGG, RSA) or autologous (MGG) protein carriers. The level of the IgG response is - using the same immunization schedule - influenced both by the bound hapten and the carrier. In both strains tested (i.e. in the high-responding A/J and the low-responding C57BL/10ScSn), the highest IgG response is elicited by FITC-BGG. The response of the C57BL/10ScSn strain is, similarly as after immunization with SRBC, approximately ten times lower. The IgG response to other antigens tested was lower in both strains and therefore the quantitative differences were less pronounced. The affinity of antibodies against the ARS and TNP determinant, detected by inhibition of plaque-forming cells, is similar in the two strains. Thus the low reactivity of the strain C57BL/10ScSn is not caused by the absence of suitable VH and VL genes, but it rather indicates a defect of some general regulatory mechanism, involved in the synthesis of IgG antibodies. After repeated administration of ARS-BGG, the antigen and the antigen - antibody complexes accumulate in high concentrations primarily in the liver of mouse strain A/J. The amount of antigen accumulated in the liver of strain C57BL/10ScSn is significantly lower.

Animals↗
PubMed1985

Phagocytosis of protamine-heparin aggregates by mouse peritoneal exudate cells.

Protamine--heparin aggregates (PHAg's) were injected intraperitoneally into thioglycollate-prestimulated mice, peritoneal exudate cells, chiefly macrophages (M phi's) being collected after 2, 15 and 60 min, 6 h, and 30 h for electron microscopy and histochemistry. An intensive phagocytosis of PHAg's was seen even after 5 min and extracellular spaces were cleared of them after 60 min. The less rapid intracellular digestion and breakdown of PHAg's were accompanied by a gradual disappearance of their outer mucopolysaccharide layer. In vitro phagocytosis (60 min in MEM) proceeded in a similar way but more slowly. Coating of PHAg's with immunoglobulin was documented in vitro by ultrastructural enzyme immunocytochemistry. Thus the exudate M phi's are capable of rapid and efficient ingestion of PHAg's which is probably supported in vivo by the interaction of PHAg surface with some exudate components. On the other hand, the intracellular digestion of PHAg's, especially of their mucopolysaccharide shell, is far more protracted.

Animals↗