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Biomedical subjects

P Rogalla

Publications and source records attributed to P Rogalla.

96 records · Page 6Linked to original sources

Current CT/MRI examination of the lower intestinal tract.

For evaluating primary colonic and rectal malignancies, CT and MRI are often complementary imaging methods which are useful in assessing patients suspected of having extensive disease and in deciding whether a patient will benefit from preoperative radiation. CT is also helpful in designing radiation ports and in detecting complications related to the neoplasm such as perforation with abscess formation. MRI offers excellent tissue resolution which aids in distinguishing between localized colorectal disease and disease which invades muscle. Also, MRI can add information with coronal views for determining whether a sphincter-saving procedure can be performed, and may be of benefit for assessing the subtle extent of tumour into muscle and bone. However, CT and MRI lack the ability to assess depth of neoplastic involvement within bowel wall. This limitation is the major factor which, combined with the inability to diagnose metastatic tumour foci in normal-sized nodes and microinvasion of perirectal fat, prevents optimal tumour staging. Because of the low accuracy for assessing early cancer stages, neither CT nor MRI are recommended for routine use in preoperative staging. CT and MRI have a premier role in the assessment of recurrent colorectal neoplasm, with CT providing a slightly better overall evaluation due to volume imaging, easy image reconstructions in different planes, and availability of excellent oral and intravenous contrast agents. Cross-sectional imaging is the only method to evaluate fully patients with total AP resection, particularly male patients. Neither CT nor MRI can determine with certainty that a soft tissue density in the surgical bed following total AP resection represents recurrent tumour unless a clear mass is present which has increased in size over time. However, both methods surpass colonoscopy for detecting early mass-like tumour recurrence at the anastomotic site due to its extrinsic component. Cross-sectional imaging plays a prominent role in assessing inflammatory disease of the colon. Clinical history, laboratory data and extent of involvement are used together with results from radiographic examinations to reach a specific diagnosis. CT is preferred over MRI in the assessment of extent of inflammatory disease in and beyond the bowel wall. An additional benefit of CT over MRI is the fact that patients with abscesses or large fluid collection can undergo drainage while still in the CT scanner. CT and MRI can aid in the distinction between ulcerative colitis with minimal wall-thickening and Crohn's disease with marked wall-thickening combined with skip lesions and fistula and/or abscess formation.(ABSTRACT TRUNCATED AT 400 WORDS)

Colonic Neoplasms↗

Evidence that metacentric and submetacentric chromosomes in canine tumors can result from telomeric fusions.

We have hypothesized that metacentric and submetacentric chromosomes frequently observed in malignant canine tumors are a result of telomeric fusions. Therefore cells from a canine mammary pleomorphic adenoma were transformed with a plasmid containing the SV40 'early region', known to cause telomeric associations. Compared with non-transformed adenoma cells, the cells had a higher proliferative capacity and expressed the large SV40-T-antigen. Karyotype studies showed the conversion from a normal to an aberrant karyotype with an increase of bi-armed chromosomes resulting from fusions of acrocentric chromosomes. In addition, the length of the telomeric repeats (TTAGGG) was determined for an early and a late passage of the transformed cells by Southern hybridization. The length of the telomeric repeats was apparently longer in the 5th than in the 38th passage. In situ hybridization with a telomere-specific DNA revealed interstitial telomeric repeats in the bi-armed chromosomes. We have concluded that these findings reflect the clonal expansion of head-to-head-telomeric fusions of canine acrocentric chromosomes leading to dicentric chromosomes with a very short distance between the two centromeres. Our results support the idea that the apparent centric fusions that have been described in some canine tumors may in fact be the cytogenetic products of head-to-head-telomeric fusions.

Adenoma, Pleomorphic↗

Dynamic and quantitative assessment of tracheomalacia by electron beam tomography: correlation with clinical symptoms and bronchoscopy.

PURPOSE: The purpose of this study was to evaluate the performance of electron beam tomography (EBT) in the dynamic and quantitative assessment of tracheal collapse in tracheomalacia. METHOD: Eight patients with suspected tracheomalacia were evaluated by EBT, and the results were correlated with both clinical symptoms and bronchoscopy. To validate this technique, an EBT phantom study preceded the patients' examination. A dynamic imaging sequence consisting of 20 50-ms scans obtained at 0.5 s intervals during a 10 s period was performed while the patient followed an instructed breathing maneuver. RESULTS: Good correlation between EBT data and clinical symptoms was found in all patients. In comparison to bronchoscopic findings, EBT results correlated well in three, showed limited correlation in three patients, and no correlation in one case. CONCLUSION: EBT with its short scanning time may be regarded as an accurate noninvasive method to dynamically evaluate tracheomalacia.

Adult↗

Regulation of the expression of HMG1, a co-activator of the estrogen receptor.

HMG1 is a protein of high clinical significance. Besides shielding of DNA adducts against repair enzymes making cells more sensitive to cisplatin therapy, it is also a co-modulator of the activity of steroid hormone-regulated genes. Although HMG1 is regulated by various factors, including steroid hormone estrogen, nothing was known about regulatory sequences. Also the sequence of parts of HMG1 including its promoter remains still unknown. We have completed the genomic organization of human HMG1 and characterized its regulatory region by luciferase assay for promoter activity. Insights into the regulation of HMG1 expression are given by the promoter analysis showing a strong functional promoter, enhancing and negative regulatory regions together with a CpG island in intron 1 and several transcription factor binding sites. Furthermore, the finding of two estrogen responsive elements within intron 1 is relevant as they indicate a direct mechanism of HMG1 up-regulation by estrogen making the presence of an estrogen receptor a significant marker for a combined treatment of special tumor types with estrogen and the anticancer drugs cisplatin or carboplatin.

5' Untranslated Regions↗

Variation of HMGB1 expression in breast cancer.

The amount of steroid hormone receptor proteins does not always correlate with the response of breast cancers to endocrine therapy. This may partly be due to the fact that binding of the estrogen receptor (ER) to estrogen responsive elements (ERE) of its target genes is mediated by additional cellular proteins. One of these is the high mobility group protein HMGB1, known to interact with ER thus dramatically increasing its binding to ERE. This is the first report analysing the expression patterns of HMGB1 in breast cancer cells. Northern blot analyses of the 1.4 kb and the 2.4 kb transcripts of HMGB1 in 13 breast cancer samples revealed a strong intertumoural variation by a factor of 8.5 and 14.5, respectively. This variation may contribute to the different response, of estrogen receptor-positive breast tumours to endocrine therapy, making HMGB1 a marker of considerable clinical interest.

Adult↗

HMGIC expression patterns in non-small lung cancer and surrounding tissue.

BACKGROUND: So far no powerful molecular markers have been found allowing an early detection of lung cancer enabling a higher rate of curative cancer therapy. Based on its expression patterns in other malignant tumors the expression of the high mobility group protein gene HMGIC is a possible new candidate for such a diagnostic marker. MATERIALS AND METHODS: The HMGIC expression patterns were determined in samples from 19 non-SCLC, 14 corresponding non-malignant adjacent lung, and 7 pleural tissues by hemi-nested RT-PCR followed by the detection of HMGIC specific amplification products by Southern blot hybridization. RESULTS: HMGIC expression was found in 14/19 non-SCLC samples. In contrast, expression was not detectable in any of the non-malignant adjacent lung or pleural tissues. CONCLUSIONS: These results suggest that HMGIC expression may be a potential new and powerful indicator for lung cancer. An HMGIC expression assay may be applicable to sputum or broncho-alveolar lavage fluid samples facilitating early detection of lung cancer.

Aged↗