Metabolism of urethane and its interaction with nucleic acids and proteins.
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Animals↗
Biomedical subjects
P Rocchi
Publications and source records attributed to P Rocchi.
Inhibitors of protein kinases induce differentiation in human neuroblastoma cell lines.
We investigated the effect on differentiation of genistein, an inhibitor of tyrosine protein kinase, and 1-(-5 isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C, in neuroblastoma cell lines. Growth inhibition and expression of morphological and biochemical properties were examined in the human neuroblastoma cell lines TS12 and SJNKP. Genistein and H7 induced neurite outgrowth, increased acetylcholinesterase activity and cell growth inhibition in both cell lines. These results underline that tyrosine protein kinase and protein kinase C may play a key role in the control of differentiation and proliferation of neural cells.
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗
DNA repair after UV and gamma irradiation. I. Rat spleen cells.
An in vitro microculture was set up to standardize DNA enzymatic repair in rat spleen lymphocytes after exposure to ultraviolet or gamma rays by measuring the tritiated thymidine uptake. A relation between irradiation dose and DNA repair is always observable when cell viability and scheduled DNA synthesis are taken into account. This micromethod utilizing rat spleen cells appears to be a suitable system for such a study since it permits a satisfactory evaluation of DNA repair.
Animals↗
DNA repair after UV and gamma irradiation. II. Human lymphocytes.
A technique of an in vitro microculture system has been set up in order to standardize DNA repair in adult human lymphocytes after ultraviolet and gamma irradiation measuring tritiated thymidine uptake. The results obtained in DNA unscheduled synthesis were different if ultraviolet or gamma rays were employed.
Cells, Cultured↗
Gamma radiation-induced differentiation on human neuroblastoma cells in culture.
We have studied the effect of gamma radiation on differentiation in neuroblastoma cell lines AF8 and SJ-N-KP. Growth inhibition and morphological and biochemical differentiation have been examined following radiation exposure to 1-10 Gy. Gamma radiation induced marked growth inhibition and morphological differentiation in a dose-and time-dependent manner in both cell lines, and induced biochemical differentiation in AF8 cells.
Acetylcholinesterase↗
Effect of butyrate analogues on proliferation and differentiation in human neuroblastoma cell lines.
Butyric acid has been shown in vitro to produce cytodifferentiation of a wide variety of neoplastic cells. The potential clinical use of this compound as a therapeutic agent is limited by its rapid metabolism. This has led to the examination, as potential antineoplastic agents, of compounds structurally correlated to butyrate, with longer biological half lives. In this study we investigated the effect in vitro of two butyrate analogues, tributyrin and butyramide, on inducing growth inhibition and expression of morphological and immunophenotypic properties, in human neuroblastoma cell lines. Treatment with tributyrin resulted in a strong inhibition of cell proliferation and in induction of extensive differentiation; on the contrary butyramide was scarcely effective or quite ineffective. These results demonstrate that tributyrin retains the effectiveness of butyrate and suggest that this analogue could have utility for cytodifferentiation therapy.
Amides↗