Search PubMed⌕ Search

Biomedical subjects

P Reilly

Publications and source records attributed to P Reilly.

At least 73 records · Page 4Linked to original sources
PubMed1982

Cholinergic modulation of substance P release.

This study was initiated to evaluate mechanisms of release of immunoreactive substance P into the peripheral circulation and to determine whether these mechanisms are subject to cholinergic modulation. In conscious dogs, a high-protein meal significantly increased plasma concentrations of immunoreactive substance P from basal levels of 13.7 +/- 1.8 pg/ml to a peak of 20.1 +/- 2.8 pg/ml after which they returned to baseline. Prior atropinization (200 mg/kg) abolished this response and lowered the plasma levels to a nadir of 2.2 pg/ml at 20 min. Similarly, infusion of bombesin (17 ng/kg/min) increased peripheral venous substance P levels by 768 +/- 155 pg-min/ml for 120 min, whereas after prior treatment with atropine, bombesin released no significant amounts of this peptide (-10 +/- 134 pg-min/ml). The data support the concept that substance P release is under cholinergic control.

Animals↗
PubMed1982

Cone lamellae and red and green rod outer segment disks contain a large intrinsic membrane protein on their margins: an ultrastructural immunocytochemical study of frog retinas.

In addition to rhodopsin, the disk membranes of rod outer segments (ROS) contain a large integral membrane protein (mol. wt 290,000). This protein was previously localized by immunocytochemistry to the margins and incisures of disks in frog red ROS by specific antibody applied to thin sections of bovine serum albumin embedded retinas (Papermaster et al., 1978b, J. Cell Biol. 78, 415-425). Upon further study of the reactions of this antibody with outer segments of other photoreceptor classes in frog retina, labeling of the short incisures and margins of green ROS and margins of cone outer segment lamellae is also observed. Thus the large protein participates in the structure of the edges of disks and lamellae of all photoreceptors in the frog. In addition, labeling of the inter-incisure surface of all photoreceptor classes was observed at high antibody concentration. In order to interpret this labeling, the effect of dilution on labeling density was determined and double reciprocal plots (Markham and Benton, 1931, J. Am. Chem. Soc. 53, 497) were employed to evaluate the relative affinity and heterogeneity. There was considerable deviation from linearity in the plots of labeling disk interiors compared to the relatively linear plots of disk incisure labeling which suggests that the interior sites contain a weakly cross-reacting antigen or that the serum contains a lower concentration of antibody weakly reactive with another antigen.

Animals↗
PubMed1982

Brain death.

Explore the source record for details and available documents.

Brain Death↗
PubMed1981

Effect of ceruletide on pituitary-hypothalamic peptides and on emotion in man.

In order to clarify the mechanism of the analgesic effect of ceruletide (CRL), the peptides B-endorphin (BE), ACTH, prolactin (PRL), growth hormone (GH) and substance P were determined in the basal state and following IV CRL administration in 11 patients. CRL, at the dose of 2 ng/kg/min, significantly augmented BE levels in the plasma, and in CSF. Substance P levels were significantly augmented by CRL in the plasma, while ACTH levels were significantly augmented in CSF. GH and PRL levels were not affected by CRL. Placebo had no effect on any of the measured peptides. The effect of CRL on mood and anxiety, known to be affected by opioids, was studied in 14 patients with psychogenic headache. The effect of histamine induced headache on State trait anxiety inventory and on Mood adjective check list was studied before and after administration of placebo or CRL. CRL significantly diminished anxiety when compared to placebo. Elation, surgency and egotism were significantly augmented while skepticism was significantly diminished by CRL. The CRL effect on mood and pain may be mediated by augmented levels of neurohormones both in the plasma and in CSF.

Adrenocorticotropic Hormone↗
PubMed1981

Radioimmunoassay measurement of substance P release following a meat meal.

We report the development of a sensitive and specific radioimmunoassay for substance P based on a new extraction technique for this peptide. In this technique, substance P-like immunoreactivity (SPLI) was extracted from midly acidified plasma successively with 0.05M ammonium sulfate and ethanol; recovery was almost quantitative (89.5 +/- 3.2%) and was independent of concentration. Basal substance P levels averaged 22 +/- 3 pg/ml in 33 dogs. Following initiation of a high-protein meal, mean SPLI levels in three dogs (in a total of 12 experiments) increased significantly from 26 +/- 3 to a peak of 37 +/- 4 pg/ml at 15 minutes, after which they slowly returned to the initial levels.

Animals↗
PubMed1979

Tissue perfusion rate determined from the decay of oxygen-15 activity after photon activation in situ.

Rates of cerebral perfusion were obtained from measurements of the disappearance (wash-out) of oxygen-15 after in situ tissue activation with 45-million-volt x-rays. In an anesthetized cat, typical values were 90 milliliters per minute per 100 grams of tissue, with 55 percent wash-out. In a specific radiotherapy patient, the value was 65 milliliters per minute per 100 grams of tissue, with 63 percent wash-out of oxygen-15 through incorporation into tissue water.

Animals↗
PubMed1979

Differential radiolabelling of lymphocyte membrane alloantigens and immunoglobulins: variation of H2O2 concentration during lactoperoxidase catalyzed cell surface radio-iodination.

The effect of H2O2 concentration on lactoperoxidase catalyzed cell surface radio-iodination and subsequent isolation of Murine splenic lymphocyte Ia, H-2K and Lyb-3 surface antigens and membrane immunoglobulins was studied. For most membrane polypeptides analyzed 0.3 mM H2O2 proved to be optimal for the recovery of radiolabelled antigens from detergent lysates of labelled cells by immunoprecipitation. Marked variations among surface antigens and membrane immunoglobulin polypeptide chains were observed for the iodination and recovery of these proteins above and below the optimal peroxide concentration. The results suggest that cell surface radio-iodination conditions should be standardized to the requirements of the particular membrane protein being studied. The differential iodination and recovery of discrete membrane components above and below optimal conditions may prove useful in the analysis of surface membrane protein structure and membrane association.

Animals↗
PubMed1978

Exercise training and plasma catecholamines in patients with ischemic heart disease.

A 3 month program of exercise training was carried out in 10 patients with ischemic heart disease to assess the contribution of catecholamines to the training effect of improved exercise capacity and enhanced myocardial oxygen delivery. Training resulted in a significant increase in maximal treadmill exercise time (mean +/- standard error the mean 374 +/- 28 versus 567 +/- 33 seconds, P less than 0.0001). The heart rate-arterial pressure product, an index of myocardial oxygen consumption, was decreased at rest and at all levels of exercise after training. After training, the mean plasma norepinephrine level during supine rest was reduced (320 +/- 23 to 191 +/- 20 pg/ml, P less than 0.01) and plasma epinephrine values were unchanged. When these previously sedentary patients exercised on a treadmill to the maximal level attained before exercise training, the mean plasma norepinephrne and plasma epinephrine concentrations were significantly reduced. These data suggest that the beneficial adaptations of the cardiovascular system to exercise training are at least partially mediated by changes in the catecholamine response to exercise.

Adult↗