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Biomedical subjects

P Pugin

Publications and source records attributed to P Pugin.

35 records · Page 2Linked to original sources
PubMed1983

Bacteriological study of amniotic fluid during labor.

Amniotic fluid from 207 women in labor was analysed at the time of artificial rupture of membranes or by amniocentesis. The following organisms were identified in concentrations of more than 1 000/ml: Staphylococcus aureus (1), Propionibacterium (1), E. coli (1), group B Streptococci (3), Lactobacilli (16). The 6 patient-carriers of pathogens became infected as did 4 of their babies. Leukocyte counts and LDH levels performed on amniotic fluid did not correlate with the appearance of symptoms of infection. Quantitative bacteriology of amniotic fluid seems to be of value in identifying patients at high risk of developing endometritis and/or neonatal sepsis.

Amniotic Fluid↗
PubMed1979

[Selective aplasia of neutrophils: auto-immune origin].

Autoimmunity is a recognized factor in pure red cell aplasia and in some cases of aplastic anemia, but not in agranulocytosis. The case reported here demonstrated that pure neutrophilic aplasia of autoimmune origin may exist in man. A 75-year-old male is described who presented with complete agranulocytosis and absence of neutrophilic precursors in the bone marrow without thrombocytopenia or anemia. After 6 weeks, remission was induced by immunosuppressive treatment and 22 months later the patient is doing well without therapy. The absence of known cases of agranulocytosis, a good response to immunosuppressive therapy, and the demonstrated fact that the patient's peripheral blood mononuclear cells inhibit the growth of granulocytic colonies of normal human marrow in vitro allow the conclusion that this patient had pure neutropenic aplasia, probably due to autoimmune phenomena.

Aged↗
PubMed1979

[Sideroblastic anemia: clinical and hematological study on 57 patients].

Sideroblastic anemias (SA) are a heterogeneous group of hematologic disorders marked by a defect of heme synthesis, disturbance of iron metabolism and the presence of ringed sideroblasts. 57 cases of SA, including 23 primary and 34 secondary forms, are discussed. In the group of secondary SA 13 patients were alcoholics, 3 had received chloramphenicol, 2 had lead poisoning, 2 had received busulfan and 2 had immune hemolytic anemia. In some cases serum ferritin was found to be excessively high. The caryotype of one patient presented deletion of the long arm on chromosome 20. None of the patients with primary SA responded to vitamin treatment, and 3 developed a myeloproliferative syndrome.

Alcoholism↗
PubMed1979

[Kala-azar; clinical and physiopathological study a propos of a new case studied in Switzerland].

Kala-azar, a parasitic disease caused by Leishmania donovani, is usually found in tropical areas but may occasionally occur in other regions such as the Mediterranean. A case of kala-azar in a women who had been on a holiday in Greece is reported. The parasite was demonstrated in lymph node biopsies and bone marrow smears and treatment with stibogluconate was begun. High levels of circulating immune complexes were demonstrable before and during the demonstration of parasites; the immune complexes were partially characterized. The diminution of complexes in serum paralleled the regression of clinical symptoms, suggesting a relationship between the clinical course and levels of circulating immune complexes. The disease may be a serum sickness-like syndrome induced by the parasite.

Adult↗
PubMed1979

Reduced leucocyte alkaline phosphatase activity and decreased NBT reduction test in induced iron deficiency anaemia in rabbits.

Iron deficiency anaemia was induced in rabbits by repeated bleeding. The leucocyte alkaline phosphatase (LAP) of 26 +/- 28 units was significantly reduced compared with control values of 233 +/- 35 units (P less than 0.001). Leucocyte NBT reduction was also diminished, both in Hanks solution (P less than 0.01) and in autologous serum (P less than 0.001). After administration of iron, these values returned to normal. The results suggest that reduced LAP may reflect a deficiency of iron dependent constituents which are necessary for the integrity of normal granulocyte metabolism.

Alkaline Phosphatase↗
PubMed1978

[Agranulocytosis and intravenous cloxacillin].

Two patients receiving parenteral cloxacillin treatment developed agranulocytosis. Upon discontinuation of the drug, the number of leukocytes rapidly returned to normal. It is likely that an immunologic mechanism may be implicated in this drug-induced blood dyscrasia.

Adult↗
PubMed1978

[Incidence and specificity of circulating immune complexes in infectious mononucleosis (proceedings)].

Occurrence of immune complexes in infectious mononucleosis has been investigated by the 125I Clq binding assay. Increased serum Clq-binding activity was found in 87% of the 23 patients studied during the acute stage of the disease. The serum Clq-binding material detected has properties identical to those of immune complexes. IgG antibodies dissociated from the complexes at acid pH and F (ab)'2 fragments obtained after treatment by pepsin appeared to be directed against the viral capsid antigen of Epstein-Barr virus.

Antibodies, Viral↗
PubMed1978

[Immunopathology in a case of kala-azar (proceedings)].

A 33 year old woman was admitted to hospital for fever of unknown origin. Leishmania donovani was found in histological preparations from lymphnodes and by sternal puncture. Circulating immune complexes present in high concentration were isolated and characterized. The circulating immune complexes remained elevated two months after the disappearance of Leishmania from the bone marrow.

Antigen-Antibody Complex↗
PubMed1978

[Pseudothrombopenia (proceedings)].

Two cases of spurious thrombocytopenia are reported, one induced by platelet satellitism and the other by platelet aggregation. These phenomena occur in vitro, only in the presence of EDTA and are linked with the presence of IgG in the patients' sera.

Aged↗
PubMed1978

[Haemoglobinosis C/beta-thalassemia double heterozygosity in an Algerian patient with total suppression of haemoglobin A synthesis (author's transl)].

The authors describe the case of a young Algerian, aged 32, suffering from mild icterus, accompanied by a marked splenomegaly. The blood count revealed a moderate degree of anaemia with reticulocytosis, pronounced anisocytosis, micro-spherocytes, bulls eye cells, folded cells, hypochrome cells, a marked polychromasia and a mild erythroblastosis. Present also were hyperbilirubinaemia, raised plasma haemoglobin, zero haptoglobin, a reduced osmotic fragility and half-life of erythrocytes. Haemoglobin electrophoresis showed 17.25% haemoglobin F, 62.8% haemoglobin C+A2 and no haemoglobin A. The genetic study indicated that the patient was a double heterozygote C/beta thalassaemia, his mother and his son both suffering from this disease. This thalassemic gene of type beta (0) totally inhibited the synthesis of haemoglobin A, the defect found in our patient.

Adult↗
PubMedDate not supplied

Is high prevalence of Echinococcus multilocularis in wild and domestic animals associated with disease incidence in humans?

We investigated a focus of highly endemic Echinococcus multilocularis infection to assess persistence of high endemicity in rural rodents, explore potential for parasite transmission to domestic carnivores, and assess (serologically) putative exposure versus infection frequency in inhabitants of the region. From spring 1993 to spring 1998, the prevalence of E. multilocularis in rodents was 9% to 39% for Arvicola terrestris and 10% to 21% for Microtus arvalis. From June 1996 to October 1997, 6 (7%) of 86 feral dogs and 1 of 33 cats living close to the region tested positive for intestinal E. multilocularis infection. Testing included egg detection by coproscopy, antigen detection by enzyme-linked immunosorbent assay (ELISA), and specific parasite DNA amplification by polymerase chain reaction. Thus, the presence of infected domestic carnivores can increase E. multilocularis exposure risk in humans. A seroepidemiologic survey of 2,943 blood donors in the area used specific Em2-ELISA. Comparative statistical analyses of seroprevalence and clinical incidence showed an increase in Em2-seroprevalence from 1986 and 1996-97 but no increase in clinical incidence of alveolar hydatid disease.

Animals↗
PubMedDate not supplied

The calcium-binding protein calretinin-22k is detectable in the serum and specific cells of cancer patients.

BACKGROUND: Calretinin-22k (CR-22k), an alternatively spliced form of calretinin (CR) belongs to the EF-hand family of calcium-binding proteins and is expressed in several colon adenocarcinoma cell lines (e.g. WiDr, HT-29). MATERIALS AND METHODS: Serum samples of cancer patients were screened with a sandwich ELISA technique using the CR-specific antiserum 7696. Highly positive samples were analyzed by Western blots and immunohistochemistry. RESULTS: CR-22k was detected in the serum of several patients and values were as high as 0.19 microgram/ml. Western blot analysis confirmed the identity of the bound protein as CR-22k. The highest concentrations were detected in patients with colon or breast cancer, but also in a patient with ischemic necrosis of the gut. CR immunoreactivity was localized to epithelial cells, nerve fibres, cells of the connective tissue and to mesothelial cells. CONCLUSIONS: Our results establish that CR-22k is detectable in the serum of cancer patients under specific pathological conditions.

Alternative Splicing↗