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P Pothier

Publications and source records attributed to P Pothier.

At least 73 records · Page 4Linked to original sources
PubMed1987

On the presence of chromaffin cells in the adrenal cortex: their possible role in adrenocortical function.

Using light and electron microscopy, we have observed the presence of rays containing medullary tissue extending across the cortex of rat adrenal glands. Within these rays chromaffin cells, as well as collagen and nerve fibers, were present. It is suggested that these endocrine cells may have a paracrine function within the cortex, possibly via their secretory product.

Adrenal Cortex↗
PubMed1987

Epidermal growth factor receptors during postnatal development of the mouse colon.

The present study was undertaken to establish the postnatal profile of specific epidermal growth factor (EGF) binding in the maturing mouse colon, with particular emphasis on possible regional differences between both proximal and distal colonic binding patterns vs. those of the small intestine. Binding studies using [125I]EGF were performed on isolated epithelial cells obtained from 2-, 5-, 9-, 16-, and 22-day-old mice as well as adults. At 2 days, cells isolated from the entire colon bound 4 times more [125I]EGF than did corresponding intestinal cells, whereas between the ages of 5 days to adult, colonic cells bound between 1.7-2.5 times more labeled EGF than their intestinal counterparts. The immature colon already exhibited maximal binding after birth as opposed to the small intestine where binding only reached maximal values by the third week of life. Comparison between the proximal and distal colon in 9-, 16-, and 22-day-old mice revealed a further 2-fold increase in EGF binding in the distal colon compared to that in the proximal colon. Scatchard plots of [125I]EGF displacement by native EGF in both proximal and distal colonic segments also revealed the presence of two classes of binding sites, with high affinity constants (K1) significantly greater in the distal colon. These results demonstrate for the first time not only the continued presence of EGF receptors in mouse colonic epithelium, but also significant regional differences in EGF-binding capacity within the digestive tract throughout the postnatal period.

Animals↗
PubMed1986

Comparison of cell culture with two direct Chlamydia tests using immunofluorescence or enzyme-linked immunosorbent assay.

Two direct tests for diagnosis of infection due to Chlamydia trachomatis were evaluated on 417 specimens collected from a population with a low disease prevalence of 8.1%. The intensity of positive results was graded according to the number of inclusions or elementary bodies and the optical density of the reaction. Thirty-four specimens were positive in cell culture, 39 positive with MicroTrak and 43 positive with Chlamydiazyme assay. The sensitivity of the two direct tests was 91.2% (31 of 34); the specificity was 97.9% (381 of 389) for MicroTrak and 96.9% (377 of 389) for Chlamydiazyme assay. The positive predictive values were 79.5% (31 of 39) for MicroTrak and 72.1% (31 of 43) for Chlamydiazyme assay. None of the specimens negative by the culture method were positive by the two direct methods. Discrepancies were restricted to the slightly positive specimens. The direct tests seem to be an alternative for diagnosing Chlamydia trachomatis infections, but slightly positive results require cell culture confirmation.

Chlamydia Infections↗
PubMed1986

Use of monoclonal antibodies for rapid detection of influenza A virus in nasopharyngeal secretions.

Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1, H3N2).

Antibodies, Monoclonal↗
PubMed1986

Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques.

The sensitivity and the specificity of two new commercial reagent tests, an indirect fluorescent antibody test (FAT) with a mouse monoclonal antibody (MAb) against respiratory syncytial virus (RSV) and an enzyme-linked immunosorbent assay (ELISA) RSV antigen detection kit, were determined by a comparison of results from these tests with those of tissue culture isolation and an indirect FAT with bovine polyclonal antibody (BPA). Of 251 nasal aspirates from infants with suspected RSV infection, positive results were found for 99 (39%) by the FAT-MAb, 93 (37%) by the FAT-BPA, and 87 (35%) by the ELISA; 69 of 240 (29%) were positive by cultures. The FAT-MAb was a more sensitive technique than cultures, with 87% sensitivity for the FAT-MAb and 84% for the ELISA. It was also more sensitive than the FAT-BPA, with 97% sensitivity for the FAT-MAb and 85% for the ELISA. This could be caused only by the distinctive volume of suspended specimens used in these tests. Of 171 negative culture specimens, positive (but not false-positive) results were found for 18% by the FAT-MAb and for 12% by the ELISA. Inversely, 13% of 69 culture positive specimens were FAT-MAb negative and 16% were ELISA negative, emphasizing the importance of tissue cultures for the maximum recovery of RSV, as well as for detection of other respiratory viruses. The FAT-MAb and ELISA were easy to perform and interpret, thus facilitating wider use.

Antigens, Viral↗
PubMed1986

[Respiratory syncytial virus infections in newborn infants].

Twenty-three of 23 neonates were contaminated in the course of an outbreak of respiratory syncytial virus (RSV) in a neonatal care unit. Symptoms among 22 infected symptomatic infants included rhinitis (n = 21), dyspnea (n = 19), cough (n = 17), apnea (n = 5), seizures (n = 3), fever (n = 3). Five patients presented with severe respiratory distress. The occurrence of non-obstructive apnea was significantly correlated with a history of respiratory disease, RSV infection during the first 15 days of life and the severity of lower respiratory tract RSV infection.

Cross Infection↗
PubMed1985

Outbreak of respiratory syncytial virus in France.

A report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in France. Twenty-three of 32 infants were infected (72%) despite infection control procedures. Prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). The administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05).

Disease Outbreaks↗
PubMed1985

Monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions.

We developed five monoclonal antibodies against respiratory syncytial virus. Three of these (23A3, 12A4, and 18B2) were used in an indirect fluorescent antibody test, and the results were compared with those of a similar indirect fluorescent test with commercial anti-respiratory syncytial virus serum. The results obtained with antibody 18B2 and commercial anti-respiratory syncytial virus serum were identical, whereas with antibodies 23A3 and 12A4 the incidence of positive identifications was around 50%.

Antibodies, Monoclonal↗
PubMed1984

Survey of human rotavirus propagation as studied by electrophoresis of genomic RNA.

Rotavirus RNA isolated from three different environmental sources during the winter of 1981-1982 was analyzed by polyacrylamide gel electrophoresis. Our results demonstrate the presence of novel RNA patterns, the coexistence of multiple electrophoretic patterns in rotavirus isolated from samples from a given ward, and that spread of a given electropherotype is limited.

Child↗
PubMed1984

Monoclonal antibodies to the major capsid protein of human papillomavirus type 1.

Two stable monoclonal hybridoma cell lines secreting type-specific antibodies against the human papillomavirus type 1 (HPV-1) were isolated. The monoclonal antibodies detected HPV-1 antigens in frozen sections of HPV-1-induced warts, using immunofluorescence or immunoperoxidase techniques, and they reacted with HPV-1 particles in an immunodiffusion test. The two monoclonal antibodies recognized the major structural viral polypeptide, with a molecular weight of 54 000, and a minor polypeptide, with molecular weight of 76 000, in both the dissociated viral particles and in the wart extracts.

Antibodies, Monoclonal↗
PubMed1981

The inhibition of vaccinia virus replication by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB): an effect at the assembly stage.

The step sensitive to DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) in vaccinia virus replication has been investigated. Ninety microM-DRB extensively inhibited the yield of vaccinia virus after infection of Ehrlich ascites tumour cells. DRB did not inhibit cytoplasmic vaccinia DNA replication. Cytoplasmic viral RNA synthesis (both early and late) was also apparently unaffected and the virus RNAs thus synthesized were normal sized. The expression of early, intermediate and late proteins was not detectably impaired by DRB in vaccinia virus-infected cells. DRB inhibited vaccinia virus replication at the assembly stage since most of the virus DNA remained in a DNase-sensitive form in the infected cells and the virus was therefore not normally coated with virus proteins.

Animals↗
PubMed1981

[Adult respiratory distress syndrome. Two post-operative cases (author's transl)].

The authors describe two post-operative Adult Respiratory Distress Syndrome (A. R. D. S.) due to Mycoplasma pneumoniae. Mycoplasma pneumoniae infection is attested by serologic arguments and isolement of Eaton Agent in throat cultures. In the first case, exists an activation of the altern way of complement. The unusual relations between Mycoplasma infection and the A. R. D. S., diagnostic and therapeutic are commented.

Adult↗