Biomedical subjects

P Poncelet

Publications and source records attributed to P Poncelet.

77 records · Page 5Linked to original sources

PubMedDate not supplied

[Microbeads and flow cytometry: how and why put the "-metry" in immuno-cytometry?].

In current practice of immunophenotyping, flow cytometers are mainly used as cell counters. These instruments which measure fluorescence intensity on each individual cell with high sensitivity can also count molecules on cells. "Putting the - metry into cytofluorometry" means measuring the expression level of molecules of biological interest on (in) target cells. Candidate molecules are numerous due to the many membrane receptors which biological function depends on the number of accessible molecules. Any modulation of the expression level brings valuable information to the biologist, beit associated to physiological differentiation, pathological state or therapeutic intervention. In order to take the best from such measurements in clinical biology, full reliability is mandatory in terms of time-to-time, platform-to-platform and lab- to-lab reproducibility. One should ban "arbitrary units" as currently provided by the instruments to switch to real units. This paper tends to review the most important features conditioning a reliable quantitation of cellular antigens, with data expressed in terms of number of molecules per cell. In addition to staining tools (antibody-based reagents) and data treatment softwares, calibration tools are mandatory. They are not all equivalent. Fluorescent beads with internal fluorescence are very useful for instrument quality-control but they can not interchange with immunological calibration systems which measure the number of antibody molecules that can bind onto the cells. Numerous application examples illustrate the interest of this "quantitative" dimension of Immunocytometry. Generalization of this approach would help getting a wider access to clinical biology.

Cytological Techniques↗

PubMedDate not supplied

[Is masked hypertension an artefact due to the blood pressure measurement method and threshold effects?].

UNLABELLED: From results of office and home measurements of blood pressure (BP), patients can be classified as "hypertensive (HT)", "normotensive (NT)", "office hypertensive (OH)" or "masked hypertensive (MH)" by crossing the classifications obtained from each method. It seems that 9 to 20% of patients could be MH with a prognosis close to HT (SHEAF study). OBJECTIVES: To test the hypothesis that at least one part of the prevalence of MH would be an artefact due to the difference between the methods of measurements (shygmomanometer vs semi-automatic device) and/or due to different definitions of office hypertension (OHT). To determine the impact of different definitions of OHT on the prevalence of MH. METHODS: During the course of a phase IV study, BP was measured with the same semi-automatic device (OMRON 705CP) both at doctor's office (3 measurements at 1-minute intervals) and at home, by the patient himself (3 measurements in the morning and in the evening at 1-minute intervals over the 7 days before the visit). Following definitions were used: Office HT: SBP > or =140 mmHg, DBP > or =90 mmHg, SBP > or =140 mmHg or DBP > or =90 mmHg; Home HT: SBP > or =135 mmHg, DBP> or =85 mmHg, SBP > or =135 mmHg or DBP > or =85 mmHg. Another definition of office HT was used SBP > or =135 mmHg, DBP > or =85 mmHg SBP > or =135 mmHg or DBP > or =85 mmHg. RESULTS: 575 patients were analysed. Results from the two methods of measurements are closed but significantly different (difference for SBP: 3.2 +/- 16.5 mmHg; p < 0.0001; difference for DBP: 1.4 +/- 10.3 mmHg; p = 0.002)

Aged↗

PubMedDate not supplied

[Use of home blood pressure devices in France in 2004].

OBJECTIVE: To estimate the number of devices for home blood pressure measurements in the French population in 2004 and to evaluate the use of these apparatus. METHODS: The French League Against Hypertension Survey 2004 (FLAHS-2004) was performed cross-sectionally on 3707 subjects, part of a sample of 5476 subjects selected as being representative of the French metropolitan population for age (35 years and above), gender, socioeconomic status, and place of living. Subjects who declare to take an antihypertensive medication were classified as treated hypertensive patients. A questionnaire evaluating the condition of use of HBP device was given. RESULTS: In 2004, 24% of the French population above the age of 35 years was treated for hypertension, corresponding to an estimate of 7.5 million of subjects. 25% of treated hypertensives and 12% of untreated subjects had a tensiometer. A total of 4 millions of BP devices are owned in the general population with 43% by treated hypertensive patients. 67% of BP devices are wrist cuff. Medical doctors recommended home blood pressure monitoring in only 12% of subjects. CONCLUSION: In 2004, the number of subjects treated for hypertension is 1/4 of the general population over the age of 35 years in France. In this group of subjects, 1/4 possessed a tensiometer but in only 10% medical doctor recommended the use of home blood pressure monitoring.

Adult↗

PubMedDate not supplied

[Changes in life style and drug therapy for treatment of hypertension in France between 2002 and 2004].

OBJECTIVES: To estimate changes in life style and drug therapy for treatment of hypertension in France between 2002 and 2004. METHODS: The French League Against Hypertension Survey 2004 (FLAHS-2004) was performed cross-sectionally on 3 707 subjects, part of a sample of 5476 subjects selected as being representative of the French metropolitan population for age (35 years and above), gender, socioeconomic status, and place of living. Subjects who declare to take an antihypertensive medication were classified as treated hypertensive patients. A questionnaire evaluating changes in life style during the last year (physical activities, quality of food consumption, smoking, and alcohol habits) was auto-administered. RESULTS: In 2004, 24% of the French population above the age of 35 years was treated for hypertension, corresponding to an estimate of 7.5 million of subjects. The two most frequent prescribed drugs are: fixed-dose combination drugs and beta-blockers in patients aged less than 75 years, and calcium antagonist and fixed-dose combination drugs in patients aged more than 75 years. Hypertensive subjects experienced: a weight loss of more than 3 kg in 11% vs 9%* in the general population, an increased in fruits and vegetables consumption in 41% vs 34%*, a decreased in cheese (21 vs 17%*), pork-butchery (46 vs 34%*) and alcohol consumption (23 vs 18%*) [*p < 0.01]. On the other hand, the percentage of subjects who increased their physical activities was low and not different in the two groups (7 vs 9%). CONCLUSION: Changes in life style were more often applied by the subjects treated for hypertension that by the general population. The dominating place that occupies today fixed-dose combination drugs indicates a change of the therapeutic practices.

Adult↗

PubMedDate not supplied

[Evaluation of 3 functional classifications of cardiac insufficiency: a national multicenter study. National College of French Cardiologists].

The scope of this study is a comparison of three functional classifications: the New York Heart Association classification, the Duke University classification, and the specific activity scale we propose. The NYHA classification is subjective, difficult to reproduce and poorly correlated to the functional capacity measured ergometrically (duration of exercise and/or VO2). A new classification must therefore be proposed. A specific activity scale adapted to our national requirements appears to be the best solution. Our study covered 15 successive days. All patients suffering from congestive heart failure who were examined over this period were included. 700 liberal cardiologists took part in this study. 2353 patients were reviewed, mean age 69 (58% male, 42% female). Heart failure was of an ichaemic origin in 37% of cases, idiopathic in 25% and due to hypertension in 25%. Symptomatology was left sided in most cases. Among NYHA Class IV patients, 75% belonged to Class IV of the specific activity scale (SAS) (23% Class III, 1% Class II), and 88% of the Duke classification (10% Class III, 1% Class II). For NYHA Class III patients, 80% were SAS Class III (5% Class IV, 13% Class II), and only 38% (42% Class IV and 16% Class II) of the Duke classification. Regarding NYHA Class II patients, 74% were SAS Class II (21% Class III and 4% Class I), and 26% of the Duke classification (39% Class I, 29% Class III and 3.6% Class IV). Finally, among NYHA Class I patients, 60% were SAS Class I (34% Class II, 5% Class III), and 74% of the Duke classification (11% Class II and 13% Class III).(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗