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Biomedical subjects

P Peng

Publications and source records attributed to P Peng.

At least 37 records · Page 2Linked to original sources
PubMed1997

Purification of immunogenic heat shock protein 70-peptide complexes by ADP-affinity chromatography.

Adenosine triphosphate (ATP)-affinity chromatography has been widely used to purify molecules of the Hsp70 family. This procedure leads to dissociation of peptides from Hsp70 molecules, resulting in Hsp70 preparations devoid of immunological activity. We report that substitution of ATP-affinity chromatography by ADP-affinity chromatography results in isolation of Hsp70 molecules which are still associated with peptides and are immunogenic. The Hsp70 preparations thus obtained contain the constitutive Hsp73 and the inducible Hsp72 molecules. These observations furnish a basis for an analysis of the structural heterogeneity among the members of the Hsp70 family and of the antigenic peptides associated with individual members of this family. They also provide a practical method for the isolation of large quantities of immunologically active Hsp70-peptide preparations.

Adenosine Diphosphate↗
PubMed1996

[The analysis of abnormal auditory brainstem responses].

The abnormal auditory brainstem responses (ABR) of 56 cases who had different retrocochlear disorders were analysed. These disease included acoustic neuroma, glioma, cholesteatoma, astrocytoma, meningoma in cerebellopontile angle (CPA), chromaffin tumor of jugular foramen, intracranial metastatic tumor, demyelinating disease, multiple cranial nerve paralysis, hepatolenticular degeneration, other CPA tumors, expansion or stenosis of internal auditory meatus (IAM), and so on. The ABR features were varied in these diseases, even in same disease. In general, the wave latencies and interwave periods prolonged in the disorders which occupied place in CPA, the differentiation of wave was not clear and/or the wave was not well repeated in the disorders which spreaded all over the brain. The cause of abnormal ABRin which CT and/or MRI were normal was also analysed. The ABR detection is a sensitive indication in retrocochlear lesions.

Cerebellar Neoplasms↗
PubMed1995

[Uvulopalatopharyngoplasty: safety managements and causes of failure].

Uvulopalatopharyngoplasty operations on 250 patients were reported. The safety managements and failing causes of UPPP were discussed. Safety managements included 5 aspects: 1. using-local anesthesia rather than general anesthesia; 2. intraoperative monitor ECG, blood pressure and oxygen saturation; 3. tracheotomy should be performed in the severe cases before UPPP; 4. preoperative physician consultation if patients complicated with cardiopulmonary problems; 5. separated nasal and UPPP operations into two stages. Failing causes included 4 aspects: 1. improper indication; 2. polyplace obstruction, specially hypopharyngeal obstruction; 3. postoperative local hemorrhage and infection; 4. the experience and the skill of the surgeon.

Adult↗
PubMed1994

[Morphology of the adult human olfactory epithelium].

The morphological observation of adult human mucous membrane has been investigated with the light microscope and T.E.M. Olfactory mucous membrane is separated into epithelium and lamina propria by basement membrane. The lamina propria contains a lot of Bowman's glands. There are many secretory granules in the cytoplasm of Bowman's gland cells. The epithelium consists of supporting cells, olfactory neurons and basal cells. Supporting cells and olfactory neurons contain plenty of mitochondria and endoplasmic reticula in the cytoplasm. The basal cell is attached to the basement membrane, few organelle in the cytoplasm. Transmission electron microscope reveals that adult human olfactory epithelium has three major changes: atypical olfactory vesicle, respiratory metaplasia and mixing of respiratory and olfactory epithelial tissues. The observations provide the new information necessary to understand the physiological mechanism and clinical disorders that affect olfactory function in humans.

Aged↗
PubMed1993

Interpreting natural language queries using the UMLS.

This paper describes AQUA (A QUery Analyzer), the natural language front end of a prototype information retrieval system. AQUA translates a user's natural language query into a representation in the Conceptual Graph formalism. The graph is then used by subsequent components to search various resources such as databases of the medical literature. The focus of the parsing method is on semantics rather than syntax, with semantic restrictions being provided by the UMLS Semantic Net. The intent of the approach is to provide a method that can be emulated easily in applications that require simple natural language interfaces.

Humans↗
PubMed1993

Generating MEDLINE search strategies using a librarian knowledge-based system.

We describe a librarian knowledge-based system that generates a search strategy from a query representation based on a user's information need. Together with the natural language parser AQUA, the system functions as a human/computer interface, which translates a user query from free text into a BRS Onsite search formulation, for searching the MEDLINE bibliographic database. In the system, conceptual graphs are used to represent the user's information need. The UMLS Metathesaurus and Semantic Net are used as the key knowledge sources in building the knowledge base.

Artificial Intelligence↗
PubMed1993

From ICD9-CM to MeSH using the UMLS: a how-to guide.

One purpose of the Unified Medical Language System (UMLS) is to facilitate conversion of terms from one controlled medical vocabulary to another. We examined our ability to convert International Classification of Diseases, 9th Edition, Clinical Modifications (ICD9-CM) to Medical Subject Headings (MeSH) using the UMLS. We describe a method which mapped 30.4% of ICD9-CM to UMLS. Of these, 95.0% were linked to MeSH, of which translation was straightforward in 90.4%. We discuss the use of these translations for retrieval from MeSH-indexed databases, such as Medline.

Disease↗
PubMed1993

Generic queries for meeting clinical information needs.

This paper describes a model for automated information retrieval in which questions posed by clinical users are analyzed to establish common syntactic and semantic patterns. The patterns are used to develop a set of general-purpose questions called generic queries. These generic queries are used in responding to specific clinical information needs. Users select generic queries in one of two ways. The user may type in questions, which are then analyzed, using natural language processing techniques, to identify the most relevant generic query; or the user may indicate patient data of interest and then pick one of several potentially relevant questions. Once the query and medical concepts have been determined, an information source is selected automatically, a retrieval strategy is composed and executed, and the results are sorted and filtered for presentation to the user. This work makes extensive use of the National Library of Medicine's Unified Medical Language System (UMLS): medical concepts are derived from the Metathesaurus, medical queries are based on semantic relations drawn from the UMLS Semantic Network, and automated source selection makes use of the Information Sources Map. The paper describes research currently under way to implement this model and reports on experience and results to date.

Algorithms↗