Biomedical subjects
P Mukherjee
Publications and source records attributed to P Mukherjee.
Anesthetic management of cleft lip and cleft palate.
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Function of prolactin cells in the individual rat pituitary gland is location dependent.
Anterior pituitary glands from individual ovariectomized (ovx) or ovx-estrogen (E2) treated rats were sectioned into 1/8 cubes. Each section was incubated for four consecutive 15 min periods in order to measure the release of immunoreactive and bioactive prolactin (PRL); each individual section was then trypsinized into a single cell suspension for determination of PRL cell numbers in that section. Hormone release (ng PRL/1000 PRL cells) was not uniform throughout the gland; the consistency of the secretory patterns demonstrated that the amount of PRL release from the gland was location-dependent. Statistical analysis of the data showed that the most active cells were in the gland's left lobe, while the least active were in the right lobe. Within these lobes, the dorsal-caudal and ventral-rostral left lobe areas released the most hormone in vitro while those in the dorsal-rostral, dorsal-caudal and ventral-rostral right lobe areas were least active. Injection of ovx rats with E2 for 2 days altered these secretory patterns. This sectioning procedure should prove useful in future studies addressing issues of cell-cell interaction and geographic location as they relate to pituitary cell function.
Prolactin-induced mitogenesis of lymphocytes from ovariectomized rats.
PRL can induce interleukin-2 (IL-2) receptor expression in splenocytes from ovariectomized (OVX) female rats. In this further study of the effects of PRL on lymphocytes in vitro we found that PRL induced IL-2 receptor expression, IL-2 production, and proliferation of splenocytes and thymocytes from OVX rats. Cells from male rats were not affected. The proliferative response, as measured by [3H]thymidine incorporation, depended on the concentration of PRL and the presence of adherent cells in the culture. After a 48-h incubation with PRL (1 microgram/ml), splenocytes from OVX rats incorporated essentially the same amount of [3H]thymidine as cells incubated with the polyclonal T-cell mitogen Concanavalin-A (ConA). As determined by autoradiography, approximately 40% of the splenocytes responded to PRL or to ConA. After incubation of splenocytes and thymocytes with PRL, bioactive IL-2 was detected in culture medium only from cells of OVX female rats, while incubation with ConA caused IL-2 production by lymphocytes from both male and OVX rats. However, ConA induced IL-2 activity sooner than PRL. Immunofluorescent-flow cytometric analysis revealed time-dependent increases in percentages of IL-2 receptor-positive splenocytes as well as increases in percentages of total T-cells and cells of the CD8 and, to a lesser extent, the CD4 subclass after PRL stimulation.
Familial fibrous dysplasia of the jaws: cherubism in two brothers.
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Interaction of T-DNA border sequences and Ti-plasmid vir functions of Agrobacterium results in differential single-stranded linear T-DNA molecule production and plant transformation.
The mechanism of Agrobacterium mediated genetic transformation of plants is dependent upon certain genetic function of the chromosome of the bacterium as well as on Ti-plasmid borne vir loci and the border sequences of T-DNA. The organisationally variable forms of the naturally occurring border sequences amongst Ti-plasmid types are differentially responsive to gene products of vir loci concerned with T-strand production. Additionally, the production of stable transformants is dependent upon vir gene products effective after T strands are produced. The interaction of border sequences from different strains of Agrobacterium with vir proteins encoded by various helper plasmids revealed that functional differences do exist amongst vir gene products contained in the type of helper plasmids used.
A family with partial androgen insensitivity.
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Some prospective observations on recent outbreak of typhoid fever in West Bengal.
The present study was designed to study the clinical behaviour of a recent epidemic of typhoid fever in West Bengal. Of 46 cases studied, 67% (31) had chloramphenicol resistant typhoid fever. The chloramphenicol-resistant cases were comparatively severe in nature with higher complication and mortality rates. Salmonella typhi resistant to chloramphenicol were also resistant to ampicillin, cloxaxillin and cotrimoxazole. Strains of Salmonella typhi sensitive to chloramphenicol retained their sensitivity to these other antimicrobials.
Short term effect of diltiazem on portal hypertension in patients with non-cirrhotic portal fibrosis.
Fourteen patients of non-cirrhotic portal fibrosis (NCPF) with portal hypertension were put on oral diltiazem hydrochloride (90 mg/day) or placebo on a prospective, randomised, single blind basis for 15 days. Predrug hemodynamic and biochemical status were similar in both groups. Diltiazem produced significant reduction (p less than 0.001) in mean intrasplenic pressure: from 41.88 (SD +/- 6.18) to 21.5 (+/- 7.91) cm of normal saline as against 45.56 (+/- 9.45) to 43.33 (+/- 8.27) in the placebo group. Mean arterial pressure (MAP), heart rate and cardiac output (CO) did not change in either group. Thus, the calcium channel blocker diltiazem reduces portal pressure in patients with NCPF, independent of reduction in MAP and CO; this is advantageous in situations where compromised cardiac hemodynamics may prove deleterious.
Antiproliferative action of retinoic acid in cultured human brain tumour cells Gl-As-14(S).
We have employed human Gl-As-14(S) brain tumour cell line to study antiproliferative action of retinoic acid (RA). RA (20 microM) caused a time-dependent, dose-dependent, cell seeding density-dependent reduction in cell proliferation in liquid medium and inhibited growth in agar. Growth inhibitory effects of RA were also affected by the concentration of fetal bovine serum (PBS) in the medium. All these effects could be reversed within 48 h after removal of RA from the growth medium. RA-treated cells also displayed reduced concanavalin A (Con A) binding ability by microhemadsorption technique. The results demonstrated that RA can suppress in this tumour cell line the expression of some properties frequently associated with transformed cells.
Prolactin induction of interleukin-2 receptors on rat splenic lymphocytes.
A case for the involvement of PRL in the regulation of the immune system is strong. However, no mechanism by which PRL exerts this regulation has yet been identified. We studied the in vitro effects of PRL on splenocytes from ovariectomized (OVX) rats and discovered that PRL induced the formation of interleukin-2 (IL-2) cell surface receptors. However, PRL did not induce IL-2 secretion. This response, which was dependent on the concentration of PRL, also depended upon the estrogen status of the splenocyte donor; thus, splenocytes from OVX rats or rats in diestrus responded to PRL, whereas those from estrogen-treated OVX rats or rats in estrus did not. We propose that in vivo exposure of PRL, under certain physiological conditions, may prime a pool of splenocytes to express IL-2 cell surface receptors, allowing these cells to be responsive to variations in local concentrations of IL-2.
Coagulation profiles in portal hypertension.
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Vascular hamartomas in infancy and childhood.
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Spontaneous neoplastic evolution of Swiss mouse embryo cells (PMS-14) in culture.
Some biological properties related to transformation have been studied to characterize a spontaneously transformed mouse embryonic fibroblast cell line, PMS-14. Low population doubling time with moderately high plating efficiency and saturation density were noted. These cells showed reduced adhesion to substratum, enhanced agglutinability by concanavalin A, anchorage independent growth in soft agar and tumorigenicity in mice.
Angular momentum saturation in the 209Bi( alpha,2n) reaction.
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3s1/2 spectroscopic strength distribution in 207Tl and 205Tl.
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Biological and growth properties of a 20-methyl-cholanthrene transformed murine fibroblast cell line (PMM-14) established from primary culture.
Some major transformation related biological and growth properties have been analysed in an in vitro, system to characterize the PMM-14 cell line, a 20-methylcholanthrene induced transformed mouse embryonic fibroblast cell line. The population doubling time of this cell line was 19 h with moderately high saturation density and plating efficiency. Attachment detachment properties showed reduced adhesion to substratum. Cytogenetic study revealed the existence of a large number of Robertsonian biarmed chromosomes with hypertriploid modal range (60-69) represented by about 21% cells. These cells showed enhanced concanavalin A agglutinability which was reduced by trypsin treatment. Its neoplastic nature was finally established by its ability to grow in agar with a high plating efficiency.
Structure of high-spin proton states in 208Pb.
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