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Biomedical subjects

P Moritz

Publications and source records attributed to P Moritz.

At least 19 recordsLinked to original sources
PubMed1998

Cloning, sequencing, and expression of the gene encoding cyclic 2, 3-diphosphoglycerate synthetase, the key enzyme of cyclic 2, 3-diphosphoglycerate metabolism in Methanothermus fervidus.

Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthermophilic methanogens. For the first time, we have purified active cDPGS from a methanogen, the hyperthermophilic archaeon Methanothermus fervidus, sequenced the coding gene, and expressed it in Escherichia coli. cDPGS purification resulted in enzyme preparations containing two isoforms differing in their electrophoretic mobility under denaturing conditions. Since both polypeptides showed the same N-terminal amino acid sequence and Southern analyses indicate the presence of only one gene coding for cDPGS in M. fervidus, the two polypeptides originate from the same gene but differ by a not yet identified modification. The native cDPGS represents a dimer with an apparent molecular mass of 112 kDa and catalyzes the reversible formation of the intramolecular phosphoanhydride bond at the expense of ATP. The enzyme shows a clear preference for the synthetic reaction: the substrate affinity and the Vmax of the synthetic reaction are a factor of 8 to 10 higher than the corresponding values for the reverse reaction. Comparison with the kinetic properties of the electrophoretically homogeneous, apparently unmodified recombinant enzyme from E. coli revealed a twofold-higher Vmax of the enzyme from M. fervidus in the synthesizing direction.

2,3-Diphosphoglycerate↗
PubMed1997

Policy issues associated with analyzing outcomes of care.

Policy questions associated with the conduct of outcomes studies are addressed and outcomes management and outcomes research are differentiated. Multiple outcomes initiatives are being undertaken in the United States, from the institutional to national and international levels. Nurses have had limited involvement, thus far-although the array of outcomes being examined is considerable. Members of an American Academy of Nursing expert panel on quality health care discuss the environmental context and multiple focuses of outcomes analysis. They provide an overview of current activities, present the challenges confronting nursing in its pursuit of a quality assessment agenda, and make recommendations for increasing nurses' participation in quality initiatives.

Health Planning Guidelines↗
PubMed1996

Comparison of plasmid DNA preparation methods for direct gene transfer and genetic immunization.

Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.

Animals↗
PubMed1991

Restriction enzymes permit quantitative determination of defined chromatin structures within the chromosome.

Open chromatin structures, operationally defined as nuclease-hypersensitive sites, are frequently found spanning the controlling regions of genes and they may ensure that trans-acting factors have ready access to their genomic substrates. The rapidity and extent of induction of a gene may be dependent on the probability that its promoter is folded into an open structure. We show that restriction enzymes can be used to estimate the probability that a given promoter region is contained within a defined structure in the chromosome. In the case of the Drosophila major heat-shock-protein gene, we show that an individual promoter element is folded in an accessible form in at least 75% of embryonic chromosomes. This efficient maintenance of the hypersensitive region may be a necessary precondition for a rapid heat-shock response.

Animals↗
PubMed1989

Nursing resources and the delivery of patient care: the National Center for Nursing Research Perspective.

The nursing shortage has preoccupied nurse executives with daily crises about how to provide adequate staff resources to assure quality care and how best to plan for the future. This major societal concern affects all settings--but most particularly hospitals. The National Center for Nursing Research (NCNR) has been actively assessing this situation and has begun several targeted initiatives. Nurse executives have a vital role in this evolving research priority area focused on nursing resources and its effect on quality of care.

Nurse Administrators↗
PubMed1976

[Morphologic changes in cryptorchism (author's transl)].

Histologic changes caused by malposition occur in the undescended testicle after the second year of life. Histologic changes like those seen in the human are found in experimental cryptorchism in dogs. The same pathologic changes also occur in the unilaterally descended testicle. The results of preliminary experiments in dogs allow the statement that in unilateral cryptorchism the damage to the contralateral, descended, testicle is produced by an autoimmunologic mechanism.

Animals↗
PubMed1976

[Round-headed spermatozoa: a rare andrologic finding ("globe-headed spermatozoa", "globozoospermia")].

The case of an infertile patient is reported who had 100% round-headed ("globe-headed") spermatozoa without acrosomes in his ejaculate. The spermatozoa were studied by light and phase contrast microscopy, as well as by transmission and--for the first time--by scanning electron microscopy. Determination of acrosin showed no enzyme activity. We propose the term "globozoospermia" for this condition which was first described in 1971. It is discussed whether it may be considered as a symptom or a distinct disease.

Acrosin↗
PubMedDate not supplied

Linking remote practice to research: technology in action.

Nursing faculty practices are rarely set up to collect and analyze data, but evidence about the effects and costs of clinical care provided by faculty is required by health plans and other payers. This article describes a strategy that links faculty research programs with practice and teaching activities in various locations. An informatics infrastructure uses a common language for data collection and a set of coherent processes for implementation. The theoretical basis for conducting practice-based research is explored.

Colorado↗